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Composite microbial preparation and application thereof in relieving depression and constipation

A technology of compounding probiotics and probiotics preparations, applied in the field of microorganisms, can solve the problems of not being able to relieve depression and constipation at the same time, unilaterally relieving mental disorders and gastrointestinal diseases, etc., so as to reduce intestinal permeability and improve intestinal permeability. Peristaltic ability, the effect of wide application prospects

Active Publication Date: 2020-10-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these patented strains only have the effect of unilaterally relieving mental disorders or gastrointestinal diseases, and cannot relieve depression and constipation at the same time

Method used

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  • Composite microbial preparation and application thereof in relieving depression and constipation
  • Composite microbial preparation and application thereof in relieving depression and constipation
  • Composite microbial preparation and application thereof in relieving depression and constipation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Isolation, screening and identification of probiotics

[0048] (1) Isolation and screening of probiotics:

[0049] (1) Draw 1 mL of feces samples collected from different regions (the feces samples are stored in 30% glycerol), and after serial dilution, spread the feces samples on the MRS solid medium, cultivate them in an anaerobic workstation for 48 hours, and anaerobically The temperature of the oxygen workstation is 37°C;

[0050] (2) Pick the bacterial strains with differences in colony morphology, separate and purify by streaking on the MRS solid medium, and record the morphological characteristics of the bacteria in detail (Table 1);

[0051] (3) Pick the single bacteria isolated from the culture medium, transfer them to MRS liquid culture medium, and culture them in an anaerobic workstation at 37°C for 48 hours, perform Gram staining on the obtained strains, and record the colony morphology;

[0052] (4) Abandon the Gram-negative bacterial strains an...

Embodiment 2

[0066] Example 2: Compound probiotics can relieve symptoms of depression and anxiety

[0067] 36 sex C57BL / 6J mice, 5 weeks old, weighing 18-20g, SPF grade. After adapting to the environment for one week, the experimental mice were randomly divided into three groups according to their body weight: normal control group, depression model group, single bacteria intervention group, and compound probiotics intervention group, with 6 mice in each group. The growth environment conditions of the experimental mice are: ambient temperature (23±2)°C, relative humidity (50±10)%, light mode (12h dark / 12h light). For the grouping and feeding scheme of experimental mice, see figure 1 .

[0068] The model was built with chronic unpredictable mild stimulation, and the specific stimulation factors included: (1) Fasting for 24 hours; (2) Water deprivation for 24 hours; (3) Clamping the tail for 3 minutes, with the clip clamping the tip of the tail for 1 cm; (4) Restraining for 2 hours; (5) ) ...

Embodiment 3

[0079] Example 3: Compound probiotics can relieve constipation symptoms caused by chronic stress

[0080] (1) The first black stool experiment

[0081] The first black stool test can be used to simulate the movement time of chyme throughout the intestinal tract. Before starting the experiment, the experimental mice were fasted for 12 hours, and then gavaged with 0.2 mL of activated carbon gum arabic aqueous solution, and each mouse was placed independently in a storage box with absorbent paper; The moment when a black stool occurs, so as to calculate the total time for passing the first black stool. The result is as Figure 4 As shown in A, the time for the depression model group and the normal control group to discharge the first black stool was 257.5 minutes and 167.8 minutes respectively. , can shorten the time of excretion of short feces by 77.8min.

[0082] (2) Small intestine propulsion rate experiment

[0083] The propulsion rate of the small intestine can reflect ...

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Abstract

The invention discloses a composite microbial preparation and application thereof in relieving depression and constipation, and belongs to the technical field of microorganisms. The prepared compositeprobiotics can reduce the concentration of adrenal release promoting hormone and corticosterone in serum of mice and relieve depression and anxiety behaviors caused by chronic pressure; the compositeprobiotics can up-regulate the expression of intestinal tract Htr4 genes, so that the intestinal tract peristalsis capacity of mice is improved; the content of short-chain fatty acid in intestinal tracts can be increased, and constipation can be remarkably improved; the gene expression of intestinal tract tight junction proteins ZO-1, Occludin and Claudin-1 can be improved, the intestinal tract permeability can be reduced, and peripheral and central nervous system inflammations can be reduced; the level of 5-HTP in intestinal tracts, serum and brains can be improved, and the potential of improving sleep is achieved. The composite probiotics prepared by the invention have wide application values in the aspects of resisting depression and anxiety, relieving constipation, resisting inflammation, improving sleep and the like.

Description

technical field [0001] The present invention relates to the application of compound microbial preparations and alleviating depression and constipation, in particular to the application of Bifidobacterium breve CCFM1025, Bifidobacterium longum subspecies infantile subspecies CCFM687 and Pediococcus lactis CCFM6432 in preparing antidepressant and anxiety, relieving constipation, anti-inflammation and improving sleep The application in pharmaceuticals and functional foods belongs to the field of microbial technology. Background technique [0002] Depression is a chronic and relapsing "spiritual cold". The occurrence of depression may be related to many factors such as parting, environmental pressure, childhood trauma, disease, and genes. Its common clinical symptoms are persistent depression, social isolation, fatigue and insomnia, and negative ideas such as self-mutilation and suicide. In clinical treatment, depressed patients are usually accompanied by various gastrointestin...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23C9/123A23C13/16A23C19/032A23C19/06A23L19/00A23L29/00A61K35/745A61K35/744A61P25/24A61P5/00A61P1/00A61P1/10A61P29/00
CPCA23L33/135A23C9/1234A23C13/16A23C19/0323A23C19/062A23L19/00A23L29/065A61K35/745A61K35/744A61P25/24A61P5/00A61P1/00A61P1/10A61P29/00A23V2002/00A23V2400/413A23V2400/519A23V2400/533A61K2300/00A23V2200/322A23V2200/32A23V2200/30
Inventor 王刚田培郡邹仁英翟齐啸陆文伟杨波崔树茂张秋香毛丙永唐鑫陈卫赵建新张灏
Owner JIANGNAN UNIV
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