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Chromogenic medium of carbapenem resisting klebsiella

A technology of penem Klebsiella and chromogenic medium, which is applied in the field of disease microorganism cultivation and identification, can solve the problems of carbapenem-resistant Klebsiella bacteria screening and other problems, and achieve early identification and accurate detection Effect

Active Publication Date: 2020-10-02
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing chromogenic culture methods on the market do not specifically screen for carbapenem-resistant Klebsiella bacteria. The closest chromogenic medium is the urethral flora localization chromogenic medium (CHROMagar, France)

Method used

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  • Chromogenic medium of carbapenem resisting klebsiella

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Preparation and detection application of carbapenem-resistant Klebsiella chromogenic medium

[0032] 1. Preparation process of carbapenem-resistant Klebsiella chromogenic medium

[0033] 1. Weigh 27.28g of Simon's citrate agar powder (containing sodium chloride 5g, magnesium sulfate 0.2g, ammonium dihydrogen phosphate 1g, dipotassium hydrogen phosphate 1g, sodium citrate 5g, agar 15g, bromothymol Blue 0.08g, pH 6.8±0.1), stirred and dissolved in 900ml of pure water, sterilized at 121°C for 15 minutes under high pressure (referring to pressure higher than standard atmospheric pressure), and then placed in an environment of 50°C to 55°C to keep warm for use.

[0034] 2. Weigh 10g of inositol, dissolve it in 100ml of pure water by heating, filter and sterilize with a 0.22um filter membrane, add it to the above sample 1, and shake it gently.

[0035] 3. Weigh 10mg of meropenem, add 1ml of sterilized pure water and mix well, then pipette 200ul and add to the ab...

experiment example 1

[0041] Experimental example 1 specificity verification

[0042] The inventor used the culture medium of Example 1 to screen and culture 5844 environmental samples collected in the ICU wards of 11 tertiary first-class hospitals; tracheal intubation, gastric tube intubation, intestinal and oral cavity samples of ICU patients 1442 cases were screened and cultured; and intestinal samples / oral samples of 207 healthy medical staff were screened and cultured. The positive screening results were accurately identified by the next-generation sequencing method, and the drug sensitivity results of the positive strains were verified by the broth dilution method.

[0043] Results: 121 strains were screened positive in 5844 environmental samples, of which 120 strains (99.17%) were carbapenem-resistant Klebsiella, 1 strain (0.83%) was carbapenem-resistant Serratia marcescens, False positives are low. Of the 120 carbapenem-resistant Klebsiella strains, 116 (96.67%) were Klebsiella pneumoniae...

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Abstract

The invention discloses a chromogenic medium of carbapenem resisting klebsiella, and belongs to the field of disease microorganism culturing and identification. The culture medium comprises 4-6 partsby weight of sodium chloride, 0.1-0.3 part by weight of magnesium sulphate, 0.5-1.5 parts by weight of ammonium dihydrogen phosphate, 0.5-1.5 parts by weight of dipotassium hydrogen phosphate, 4-6 parts by weight of sodium citrate, 13-17 parts by weight of agar, 0.06-1 part by weight of bromothymol blue, 9-11 parts by weight of myo-inositol, 0.001-0.003 part by weight of meropenem and 900-1100 parts by weight of water. The culture medium is good in specificity, quickly cultures the carbapenem resisting klebsiella, and facilitates infection control of the carbapenem resisting klebsiella in hospitals.

Description

technical field [0001] The invention belongs to the field of disease microorganism cultivation and identification. Background technique [0002] Klebsiella, belonging to the Enterobacteriaceae family, is a Gram-negative stubby bacillus. Single or in short chains, immobile, with obvious capsules. Klebsiella has strong resistance to the outside world and is prone to develop resistance to most antibiotics. [0003] Over the past 10 years, carbapenem-resistant drugs have been considered the last line of defense in the treatment of drug-resistant Gram-negative bacteria. With the rapid increase in the detection rate of carbapenem-resistant strains, especially Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa, it has become a difficult problem in clinical anti-infection treatment today. According to the latest antimicrobial resistance monitoring results of CHINET, among the 8274 strains of carbapenem-resistant Enterobacteriaceae, the top three isolation ...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/22
CPCC12Q1/045G01N2333/26
Inventor 卫丽宗志勇刘丽娜乔甫林吉
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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