Method for improving sclerotiniose resistance of Chinese cabbages

A technology of Chinese cabbage and sclerotinia, which is applied in the field of improving the resistance of Chinese cabbage to sclerotinia, can solve problems such as difficult treatment, and achieve the effects of reducing economic losses, reducing usage, and low input costs

Pending Publication Date: 2020-09-29
ZHEJIANG FORESTRY UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Due to the wide host range and long-term survival of resting sclerotia, treatment is difficult, and there is no effective method for the control of S. sclerotiorum in Chinese cabbage

Method used

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  • Method for improving sclerotiniose resistance of Chinese cabbages
  • Method for improving sclerotiniose resistance of Chinese cabbages
  • Method for improving sclerotiniose resistance of Chinese cabbages

Examples

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Embodiment 1

[0024] The method for improving the resistance of Chinese cabbage to sclerotinia disease according to the present invention includes the following steps:

[0025] (1) Preparation of Chinese cabbage seedlings: The test material was "Zaoshu No. 5" Chinese cabbage, which was purchased from Wenzhou Xianfeng Seed Co., Ltd. Sow the Chinese cabbage seeds in a petri dish lined with soaked filter paper at 25°C for 24 hours, and then sown them in the substrate. When they grow to 1-2 true leaves, select seedlings with consistent growth and transplant them into plastic pots ( 6cm×8cm×9cm).

[0026] The culture conditions are: under natural light, the average daily temperature is 25-30°C / 15-18°C (day / night) 1 Relative humidity fluctuates about (85±5)% under the condition of a photoperiod of 16h / 8h, the maximum The light intensity is 600μmol·m-2s-1.

[0027] (2) Configure the melatonin solution: first mix ethanol and water to make a 0.1% ethanol aqueous solution to make constant volume, then add...

Embodiment 2

[0030] The materials and preparation process are basically the same as those of Example 1, except that the concentration of melatonin in the melatonin solution is 100 μM.

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Abstract

The invention belongs to the technical field of plant growth regulation and control, and particularly relates to a method for improving sclerotiniose resistance of Chinese cabbages. The method comprises the following steps: step 1, mixing melatonin and a solvent to prepare a melatonin solution, wherein the concentration of melatonin in the melatonin solution is 50-100 [mu] M; and step 2, under thecondition of no illumination, spraying the prepared melatonin solution to the leaf surfaces of the seedlings of the Chinese cabbages so that the defects in the prior art are overcome; the resistanceof Chinese cabbages to sclerotinia sclerotiorum can be improved in a short time, the sclerotinia sclerotiorum morbidity condition of the Chinese cabbages can be remarkably reduced, development of sclerotinia sclerotiorum is inhibited, growth of Chinese cabbage seedlings is promoted, the use amount of pesticide is reduced, and the method can be applied to many fields such as agricultural production, scientific research and environmental protection.

Description

Technical field [0001] The invention belongs to the technical field of plant growth regulation and control, and specifically relates to a method for improving the resistance of Chinese cabbage to sclerotinia disease. Background technique [0002] Sclerotinia sclerotiorum is a necrotizing fungal plant pathogen that can infect more than 500 plants in 75 families in the world. It is one of the most nonspecific, omnivorous and successful plant pathogens (PankajSharma et al., 2016). Streptococcus sclerosus was first discovered from infected sunflowers in 1861 (BaileyKL et al., 1996). Brassica crops are the main hosts of Streptococcus sclerotiorum (GulyaT et al., 1997). The yield and quality of these crops are reduced by the disease, which may cost millions of dollars each year (BaileyKL et al., 1996; SharmaP et al., 2015). Shaw and Ajrekar published the first report on rapeseed mustard stem rot (SaharanGS et al., 2008). In Australia, rapeseed production is threatened by production...

Claims

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Application Information

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IPC IPC(8): A01N43/38A01P21/00A01G13/00
CPCA01G13/00A01N43/38
Inventor 臧运祥滕芝妍郁有健
Owner ZHEJIANG FORESTRY UNIVERSITY
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