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Method for in-vivo detection of small fish epidermal cell potassium ion flow

A technology for epidermal cell and living body detection, which is used in measurement devices, material analysis by electromagnetic means, instruments, etc., to achieve good stability and good repeatability.

Pending Publication Date: 2020-09-25
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few reports on the application of this technique to the detection of potassium ion currents in epidermal cells of small freshwater fish

Method used

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  • Method for in-vivo detection of small fish epidermal cell potassium ion flow
  • Method for in-vivo detection of small fish epidermal cell potassium ion flow
  • Method for in-vivo detection of small fish epidermal cell potassium ion flow

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Experimental program
Comparison scheme
Effect test

Embodiment

[0026] 1.1 Anesthetize the mosquito fish (average body weight (0.18±0.04) g, average body length (2.7±0.2) cm) for 10-20 min, and the anesthetic agent is 0.4 mg L –1 Ethyl m-aminobenzoate methanesulfonate (MS-222), then placed in a sterile petri dish with a diameter of 7.5cm, fixed the fish body with a filter paper strip, pressed the edge of the filter paper strip lightly with the resin block, and injected the test buffer solution, soaked for 10-30 minutes, so that the exchange of potassium ions in the epidermal cells of the fish body reached equilibrium.

[0027] 1.2 Place the soaked fish body in a new test buffer, place it under the microscope of the non-damaging micro-measurement system, observe the edge of the epidermis under the microscope, adjust it on the monitor until the epidermal cells of the fish body can be seen clearly, and look for the cells with complete cell structure. area, and then put the sensor, use the manipulator to make the sensor 1 μm away from the fish...

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Abstract

The invention discloses a method for in-vivo detection of small fish epidermal cell potassium ion flow. According to the method, the potassium ion flow of epidermis cells of the fish body is measuredby a non-invasive micrometric technology, the flow rate of potassium ions is negative, and the potassium ions in the epidermis of the fish body are internal flow. The method can realize detection of the potassium ion flow of the epidermal cells of the small fish, has the advantages of living body detection, no damage to the fish body, rapidness, short time consumption, high accuracy and the like,solves the problem that the potassium ion signal of the epidermal cells of the small fish cannot be detected in vivo in the prior art, and has certain practical value.

Description

technical field [0001] The invention belongs to the field of fish biotechnology, and in particular relates to a method for detecting potassium ion flow of epidermal cells of small fish in vivo. The method involves small freshwater fish. Background technique [0002] As a very important signaling molecule, potassium ions can not only regulate various physiological activities in fish epidermal cells, but also participate in the response process of cells to the external environment. Potassium ion signals are mainly generated by changes in the distribution and concentration of potassium ions in the cytoplasm and organelles. Various biotic or abiotic stresses in animals can cause instantaneous changes in potassium ion concentration. [0003] Non-invasive micro-measurement technology is a technology for real-time measurement of the flow rate of ions and small molecules entering and leaving the living body, and it is one of the best tools for studying the physiological functions ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/00
CPCG01N27/00
Inventor 曾鸿鹄闫小雨宋晓红黄亮亮黄月群覃礼堂
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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