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Preparation method of a mesoporous silica complex with drug controlled release and imaging functions

A technology of mesoporous silica and drug controlled release, applied in the fields of nanomaterials and molecular medicine, can solve the problems of difficult real-time monitoring of curative effect, complicated implementation, etc., and achieve the effects of high sensitivity, reduced multidrug resistance, and strong characteristics.

Pending Publication Date: 2020-09-25
CHONGQING UNIV CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since gold nanoparticles are not magnetic, non-invasive real-time drug monitoring with magnetic particles is not possible, and it is difficult to monitor the curative effect in real time; and the drug release needs to be excited by laser, which is complicated to implement and difficult in practical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This embodiment 1 provides a method for preparing a mesoporous silica complex with both controlled drug release and imaging functions, the method comprising the following steps:

[0027] (1) Au / Fe 3 o 4 Nucleus preparation

[0028] Fe 3 o 4 Preparation of nanoparticles. 1g FeCl 3 ·6H 2 O and 0.37 g FeCl 2 4H 2 O mixed with 20mL deionized water for nitrogen deoxygenation; take 130mL deionized water in a 250mL round bottom flask for nitrogen deoxygenation, add 12.5mL of concentrated ammonia water with a concentration of 25-28%, and quickly pour it into it under vigorous stirring Pour the above-mentioned iron salt mixed solution, and react for 1 h (80°C) under nitrogen; after the reaction is completed, separate Fe from the reaction solution with a permanent magnet. 3 o 4 Magnetic nanoparticles, washed three times with methanol, were dispersed in 50 mL of toluene. Then, (3-aminopropyl)trimethoxysilane (APTES) (25 μL) was added to 12.5 mL of the above Fe 3 o 4 I...

Embodiment 2

[0037] This Example 2 provides a method for preparing a mesoporous silica complex with both drug controlled release and imaging functions, the method comprising the following steps:

[0038] (1) Au / Fe 3 o 4 Nucleus preparation

[0039] Fe 3 o 4 Preparation of nanoparticles. 1g FeCl 3 ·6H 2 O and 0.37 g FeCl 2 4H 2 O mixed with 30mL deionized water for nitrogen deoxygenation; take 160mL deionized water in a 250mL round bottom flask for nitrogen deoxygenation, add 15mL of concentrated ammonia water with a concentration of 25-28%, pour it into it quickly under vigorous stirring Add the above-mentioned iron salt mixed solution, and react for 2h (80°C) under nitrogen; after the reaction is completed, use a permanent magnet to separate Fe from the reaction solution. 3 o 4 Magnetic nanoparticles, washed three times with methanol, were dispersed in 60 mL of toluene. Then, (3-aminopropyl)trimethoxysilane (APTES) (20 μL) was added to 12.5 mL of the above Fe 3 o 4 In the sol...

Embodiment 3

[0047] This embodiment 3 provides a method for preparing a mesoporous silica complex with both drug controlled release and imaging functions, the method comprising the following steps:

[0048] (1) Au / Fe 3 o 4 Nucleus preparation

[0049] Fe 3 o 4 Preparation of nanoparticles. 1g FeCl 3 ·6H 2 O and 0.37 g FeCl 2 4H 2O mixed with 20mL deionized water for nitrogen deoxygenation; take 130mL deionized water in a 250mL round bottom flask for nitrogen deoxygenation, add 12.5mL of concentrated ammonia water with a concentration of 25-28%, and quickly pour it into it under vigorous stirring Pour the above-mentioned iron salt mixed solution, and react for 1 h (80°C) under nitrogen; after the reaction is completed, separate Fe from the reaction solution with a permanent magnet. 3 o 4 Magnetic nanoparticles, washed three times with methanol, were dispersed in 50 mL of toluene. Then, (3-aminopropyl)trimethoxysilane (APTES) (25 μL) was added to 12.5 mL of the above Fe 3 o 4 In...

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Abstract

The invention discloses a preparation method of a mesoporous silica complex with drug controlled release and imaging functions. The preparation method comprises the following steps of (1) preparing anAu / Fe3O4@mSiO2-SH molecular probe; firstly, preparing Au nanoparticles; secondly, preparing Au / Fe3O4 nano particles with a shell-core structure; then, preparing Au / Fe3O4@mSiO2-SH nanoparticles by applying a reversed-phase microemulsion method; (2) preparing Au / Fe3O4@mSiO2 / DOX-SS-PEG-HA; adding Au / Fe3O4@mSiO2-SH nanoparticles into a DOX ethanol solution, performing dispersing of the obtained nanoparticles into a DMSO aqueous solution, and adding SH-PEG-NH2, so as to obtain nanoparticles Au / Fe3O4@mSiO2 / DOX-SS-PEG-NH2; enabling HA to be dissolved in an MES solution through a carbodiimide method,enabling EDC and NHS to be added to serve as coupling agents, and enabling Au / Fe3O4@mSiO2 / DOX-SS-PEG-NH2 to be added. The nanoprobe has three imaging functions of MRI, MPI and PAI and chemotherapy and photo-thermal treatment functions, can realize comprehensive, sensitive and targeted imaging of tumors, can perform real-time quantitative in-vivo monitoring of drug curative effects, and opens up abrand new field for accurate diagnosis and treatment of tumors at the molecular level.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and molecular medicine, and in particular relates to a composite mesoporous silicon dioxide nano drug carrier and a preparation method thereof. Background technique [0002] With the improvement of tumor diagnosis and treatment technology, precise treatment and monitoring of treatment response are becoming more and more important for improving efficacy. At present, the commonly used clinical tumor diagnosis and evaluation methods mainly include CT, magnetic resonance (MRI), ultrasound, etc., but there are disadvantages such as low sensitivity and specificity, and incapability of real-time monitoring. Multimodal imaging technology can overcome the limitations of existing methods, realize non-invasive, real-time, quantitative and specific detection, provide more comprehensive and accurate information and guide treatment. Chemotherapy is an important means of anti-tumor treatment, but traditio...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K49/00A61K49/08A61K49/12A61K49/18A61K49/22A61K31/337A61K41/00A61K47/60A61K47/61A61P35/00B22F1/00B22F9/24B82Y40/00
CPCA61K41/0052A61K31/337A61K47/6949A61K47/60A61K47/61A61K49/0002A61K49/08A61K49/126A61K49/183A61K49/186A61K49/1863A61K49/225A61K49/221A61K49/0021A61K49/0054A61K49/005A61P35/00B22F9/24B82Y40/00B22F1/07B22F1/054A61K2300/00
Inventor 房慧颖蒋伟
Owner CHONGQING UNIV CANCER HOSPITAL
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