Medicine for relieving alcoholic intestinal injury
A technology for alcoholic liver damage and intestinal damage, applied in the field of microorganisms, can solve the problems of slow effect and low safety, and achieve the effect of improving pathological damage, high safety and relieving alcoholic liver damage
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preparation example Construction
[0052] The preparation method of the lactobacillus suspension involved in the following examples is as follows:
[0053] Streak the Lactobacillus on the MRS solid medium and culture it at 37°C for 48 hours to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, culture it at 37°C for 18 hours for activation, and activate two generations continuously to obtain Activation solution; the activation solution was inoculated in MRS liquid medium at an inoculum size of 2% (v / v), and cultured at 37°C for 18 hours to obtain a bacterial solution; the bacterial solution was centrifuged at 4°C and 6000g for 10 minutes, The Lactobacillus cells were obtained; the Lactobacillus cells were washed 3 times with normal saline and then resuspended in a skimmed milk powder solution with a concentration of 120g / L to a bacterial concentration of 1×10 9 CFU / mL to obtain a bacterial suspension, and store the bacterial suspension at -80°C until use.
Embodiment 1
[0054] Example 1: Screening and identification of Lactobacillus rhamnosus CCFM1119
[0055] 1. Screening
[0056] Take fresh feces from healthy people in Kunshan City, Jiangsu Province as the sample. After pretreatment, the sample is stored in a -80°C refrigerator in about 30% glycerin. .9% normal saline for gradient dilution, select the appropriate gradient dilution to spread on the MRS solid medium, culture at 37°C for 48 hours, pick typical colonies to streak and purify on the MRS plate, pick single colonies and transfer to the liquid The MRS liquid medium was enriched and preserved in 30% glycerol to obtain strain CCFM1119 (the original number of the strain was JS-SZ-2-1).
[0057] 2. Identification
[0058] The genome of CCFM1119 was extracted, and the 16SrDNA of CCFM1119 was amplified and sequenced (completed by Shanghai Sangon Bioengineering Co., Ltd.). After sequencing analysis, the 16S rDNA sequence of the bacterial strain was shown in SEQ ID NO.1. The sequence was ...
Embodiment 2
[0059] Example 2: Effect of Lactobacillus rhamnosus CCFM1119 on alcohol-induced mice
[0060] Specific steps are as follows:
[0061] 1. Experimental method
[0062] Fifty 8-week-old male C57BL / 6 mice of SPF grade were randomly divided into 5 groups, 10 mice in each group, and the 5 groups were: blank control group (pair-fed, PF), alcohol-fed , AF), Lactobacillus casei V-CQRC7-161-M2 intervention group (AF+V-CQRC7-161-M2) administered intragastrically with Lactobacillus casei V-CQRC7-161-M2 bacterial suspension, intragastrically administered rhamnose milk The Lactobacillus rhamnosus CCFM1119 intervention group (AF+CCFM1119) of the bacillus CCFM1119 bacterial suspension, the Lactobacillus rhamnosus 8M-10 intervention group (AF+8M-10) of the Lactobacillus rhamnosus 8M-10 bacterial suspension ); wherein, Lactobacillus casei V-CQRC7-161-M2 and Lactobacillus rhamnosus 8M-10 are other lactobacilli screened out in the same batch as Lactobacillus rhamnosus CCFM1119.
[0063] The ex...
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