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Medicine for relieving alcoholic intestinal injury

A technology for alcoholic liver damage and intestinal damage, applied in the field of microorganisms, can solve the problems of slow effect and low safety, and achieve the effect of improving pathological damage, high safety and relieving alcoholic liver damage

Active Publication Date: 2020-09-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these drugs all have defects such as slow effect and low safety, and have certain limitations in clinical efficacy.

Method used

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  • Medicine for relieving alcoholic intestinal injury
  • Medicine for relieving alcoholic intestinal injury
  • Medicine for relieving alcoholic intestinal injury

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0052] The preparation method of the lactobacillus suspension involved in the following examples is as follows:

[0053] Streak the Lactobacillus on the MRS solid medium and culture it at 37°C for 48 hours to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, culture it at 37°C for 18 hours for activation, and activate two generations continuously to obtain Activation solution; the activation solution was inoculated in MRS liquid medium at an inoculum size of 2% (v / v), and cultured at 37°C for 18 hours to obtain a bacterial solution; the bacterial solution was centrifuged at 4°C and 6000g for 10 minutes, The Lactobacillus cells were obtained; the Lactobacillus cells were washed 3 times with normal saline and then resuspended in a skimmed milk powder solution with a concentration of 120g / L to a bacterial concentration of 1×10 9 CFU / mL to obtain a bacterial suspension, and store the bacterial suspension at -80°C until use.

Embodiment 1

[0054] Example 1: Screening and identification of Lactobacillus rhamnosus CCFM1119

[0055] 1. Screening

[0056] Take fresh feces from healthy people in Kunshan City, Jiangsu Province as the sample. After pretreatment, the sample is stored in a -80°C refrigerator in about 30% glycerin. .9% normal saline for gradient dilution, select the appropriate gradient dilution to spread on the MRS solid medium, culture at 37°C for 48 hours, pick typical colonies to streak and purify on the MRS plate, pick single colonies and transfer to the liquid The MRS liquid medium was enriched and preserved in 30% glycerol to obtain strain CCFM1119 (the original number of the strain was JS-SZ-2-1).

[0057] 2. Identification

[0058] The genome of CCFM1119 was extracted, and the 16SrDNA of CCFM1119 was amplified and sequenced (completed by Shanghai Sangon Bioengineering Co., Ltd.). After sequencing analysis, the 16S rDNA sequence of the bacterial strain was shown in SEQ ID NO.1. The sequence was ...

Embodiment 2

[0059] Example 2: Effect of Lactobacillus rhamnosus CCFM1119 on alcohol-induced mice

[0060] Specific steps are as follows:

[0061] 1. Experimental method

[0062] Fifty 8-week-old male C57BL / 6 mice of SPF grade were randomly divided into 5 groups, 10 mice in each group, and the 5 groups were: blank control group (pair-fed, PF), alcohol-fed , AF), Lactobacillus casei V-CQRC7-161-M2 intervention group (AF+V-CQRC7-161-M2) administered intragastrically with Lactobacillus casei V-CQRC7-161-M2 bacterial suspension, intragastrically administered rhamnose milk The Lactobacillus rhamnosus CCFM1119 intervention group (AF+CCFM1119) of the bacillus CCFM1119 bacterial suspension, the Lactobacillus rhamnosus 8M-10 intervention group (AF+8M-10) of the Lactobacillus rhamnosus 8M-10 bacterial suspension ); wherein, Lactobacillus casei V-CQRC7-161-M2 and Lactobacillus rhamnosus 8M-10 are other lactobacilli screened out in the same batch as Lactobacillus rhamnosus CCFM1119.

[0063] The ex...

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Abstract

The invention discloses a medicine for relieving alcoholic intestinal injury, and belongs to the technical field of microorganisms and medicines. The invention provides a medicine capable of preventing and / or treating alcoholic intestinal injury or alcoholic liver injury. The effective component of the medicine is Lactobacillus rhamnosus CCFM1119, and the Lactobacillus rhamnosus CCFM1119 can effectively relieve alcoholic intestinal injury and alcoholic liver injury, and is specifically embodied in (1) significantly improving the mRNA expression level of intestinal tight junction protein of analcohol-molded mouse; (2) significantly reducing the content of endotoxin (LPS) in serum of the alcohol-molded mouse; (3) significantly reducing the activity of alanine Aminotransferase (ALT) and aspartate Aminotransferase (AST) in serum of the alcohol-molded mouse; and (4) significantly improving pathological injury of liver tissue of the alcohol-molded mouse.

Description

technical field [0001] The invention relates to a medicine for alleviating alcoholic intestinal damage, and belongs to the technical field of microbes and the technical field of medicine. Background technique [0002] In recent years, with the continuous improvement of people's living standards, the per capita consumption of alcohol in my country has increased significantly, and the incidence of alcohol-related organ damage diseases has also increased year by year. [0003] Intestinal tract is one of the important target organs of alcohol injury. Alcoholic intestinal injury will cause the dysfunction of human intestinal mucosal epithelial cells, and then change the permeability of human small intestine, which will eventually lead to high resistance to pathogenic factors such as endotoxin and bacteria in human small intestine. permeability. The high permeability of the small intestine to pathogenic factors such as endotoxin and bacteria can lead to fever, leukocytosis, micro...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P1/00A61P1/16
CPCA61K35/747A61P1/00A61P1/16Y02A50/30
Inventor 翟齐啸陈卫朱诗雅于雷雷田丰伟赵建新张灏
Owner JIANGNAN UNIV
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