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Biomimetic nanomaterials and preparation methods thereof for sonodynamic/gas synergistic anti-tumor therapy

A biomimetic nano-acoustodynamic technology, applied in the field of biomimetic nano-materials, can solve the problems of low tumor enrichment efficiency, poor biocompatibility of nanocarriers, and reduced treatment efficiency, so as to improve enrichment efficiency and biocompatibility , Reduce the effect of phagocytosis and clearance

Active Publication Date: 2022-04-12
MENGCHAO HEPATOBILIARY HOSPITAL OF FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The current nanocarriers have problems such as poor biocompatibility, low tumor enrichment efficiency, and easy recognition by the body's immune system, which reduces its therapeutic efficiency.

Method used

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  • Biomimetic nanomaterials and preparation methods thereof for sonodynamic/gas synergistic anti-tumor therapy
  • Biomimetic nanomaterials and preparation methods thereof for sonodynamic/gas synergistic anti-tumor therapy
  • Biomimetic nanomaterials and preparation methods thereof for sonodynamic/gas synergistic anti-tumor therapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Measure 6 mL of DMF dispersion containing 0.2 mg / mL black phosphorus nanosheet BPNs, in an ice-bath environment, use a cell sonicator at 70% output power for 12 h, and centrifuge at 2000 r / min for 5 min to remove The precipitate (that is, the BPNs that has not been stripped by ultrasound) was centrifuged in an ultracentrifuge at 40,000 r / min for 20 min, and the precipitate was taken to obtain 1.2 mg of black phosphorus quantum dots (BPQDs), which were resuspended in 1 mL of deionized water.

Embodiment 2

[0033] Weigh 2 g CTAC and 20 mg TEA respectively, add both to the BPQDs suspension prepared in Example 1, stir vigorously for 1 hour, then slowly drop 1.5 mL TEOS into the above mixed solution, and vigorously Stir for 1 hour, and after the solution drops to room temperature, centrifuge for 10 min at 12,000 r / min, take the precipitate and wash it with ethanol three times, then disperse it in 50 mL of methanol containing 1 wt% sodium chloride, stir for 3 hours, and centrifuge. The precipitation was repeatedly washed and stirred three times to remove the template CTAC and obtain black phosphorus quantum dot hybrid mesoporous silicon nanoparticles (BMSN).

[0034] Add the prepared BMSN and APTES to ethanol at a mass ratio of 5:1, stir vigorously at 80°C for 24 hours, centrifuge, wash the precipitate with ethanol three times, and resuspend with deionized water to obtain BMSN with a concentration of 3 mg / mL -NH 2 solution. 2 mL of BMSN-NH 2 The solution was mixed with 5 μL, 20 mg...

Embodiment 3

[0037] To lyse the J774A.1 cells, that is, to culture the J774A.1 cells in a culture dish first, and scrape them off with a cell scraper after the cells are congested; transfer the cells to a centrifuge tube after adding a small amount of PBS, and centrifuge at 300 g for 5 min to get the cell pellet, then add PBS to wash twice; then follow the operation steps of the membrane protein extraction kit (purchased from Beyontian Biotechnology Co., Ltd.) to extract the cell membrane, and store the extracted J774A.1 cell membrane at -80 ℃ spare. After BCA protein quantification, CAu-BMSN was mixed with macrophage cell membrane at a mass ratio of 5:1 and sonicated for 30 min to prepare N@CAu-BMSN.

[0038] The resulting N@CAu-BMSN was characterized by transmission electron microscopy, and detected by Coomassie brilliant blue and Western blot. The results are as follows figure 2 shown. Depend on figure 2 It can be seen from the middle transmission electron microscope image that the...

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Abstract

The present invention provides a novel biomimetic nanomaterial for acoustic power / gas synergistic anti-tumor therapy, which mainly utilizes black phosphorus quantum dot hybrid mesoporous silicon nanoparticles to load gold nanoparticles (AuNPs) in situ, and utilizes Its rich pore structure is advantageous for loading CO gas prodrug molecule CORM‑401, and further using macrophage membrane for coating to obtain the biomimetic nanomaterial N@CAu‑BMSN. The utilization of the macrophage cell membrane in the biomimetic nanomaterial can effectively avoid the phagocytosis and clearance of N@CAu‑BMSN by macrophages in the body, and improve the enrichment of the nanomaterial in the tumor area; at the same time, under the intervention of local ultrasound, the nanomaterial The inner core can effectively generate CO gas in situ in tumor cells and 1 o 2 It is used to induce tumor cell apoptosis, activate the body's immune system, and realize sonodynamic / gas synergistic anti-tumor therapy.

Description

technical field [0001] The invention belongs to the field of medical materials, and in particular relates to a biomimetic nanomaterial (N@CAu-BMSN) which can be used for sonodynamic / gas synergistic anti-tumor therapy and its preparation and application. Background technique [0002] According to statistics, there are about 3.929 million new cancer cases and about 2.338 million deaths in the country every year. Malignant tumors have become one of the major public health problems that seriously threaten the health of the Chinese population. The treatment of malignant tumors is a worldwide problem that needs to be solved urgently, and has extremely important strategic significance. Conventional surgical resection, radiotherapy, chemotherapy and even biological targeted therapy all have certain limitations, which keep the rate of tumor recurrence and metastasis high. [0003] Ultrasound has unique advantages such as unlimited penetration depth, uniform distribution in tissues,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K9/50A61K33/24A61K47/69A61K47/46A61K49/00A61P35/00B82Y5/00
CPCA61K41/0033A61K33/24A61K47/6949A61K9/5068A61K49/0067A61K49/0097A61K49/0093A61P35/00B82Y5/00A61K2300/00
Inventor 吴名张达刘小龙刘景丰曾永毅
Owner MENGCHAO HEPATOBILIARY HOSPITAL OF FUJIAN MEDICAL UNIV
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