Method for enriching and separating perchloroethylene (PCE) dechlorinating bacteria and application

A technology for enriching tetrachloroethylene and tetrachloroethylene, which is applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc. effect of survival

Inactive Publication Date: 2020-09-18
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention aims at the problem that the abundance of most Dehalococcoides in the in situ environment is low, and the traditional strain separation technology cannot achieve high-efficiency enrichment and separation of PCE dechlorination microorganisms, and aims to provide a high-efficiency enrichment and separation of tetrachlorethylene complete dechlorination The method and application of chlorine bacteria; the present invention realizes the high-efficiency enrichment culture of tetrachlorethylene complete dechlorination bacteria, and the dechlorination mixed bacteria obtained by the enrichment method of the present invention can completely dechlorinate PCE to produce ethene on the 26th day, showing Excellent dechlorination effect was obtained, and the abundance of Dehalococcoides in the dechlorinated mixed bacteria reached 68.87%; and further using ecological principles, the high-efficiency PCE dechlorinated bacteria were obtained from the enriched dechlorinated mixed bacteria: Geobacter Geobacter

Method used

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  • Method for enriching and separating perchloroethylene (PCE) dechlorinating bacteria and application
  • Method for enriching and separating perchloroethylene (PCE) dechlorinating bacteria and application
  • Method for enriching and separating perchloroethylene (PCE) dechlorinating bacteria and application

Examples

Experimental program
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Effect test

Embodiment 1

[0063] Example 1 High-efficiency enrichment of tetrachlorethylene complete dechlorination mixed bacteria from urban black and odorous water

[0064] 1. Experimental method

[0065] (1) Enrichment method

[0066] Take 5 mL of sediment samples collected from urban PCE-polluted black and odorous water, and inoculate them into DL-sodium lactate anaerobic dechlorination liquid medium with 1 mM PCE as the only electron acceptor (its formula is shown in Table 1). The impact of oxygen, to ensure the complete anaerobic culture environment, add excess Na to the medium 2 S. The culture temperature was 30°C, and the dechlorination of PCE was monitored at the same time to observe whether it produced ethene. After the PCE in the culture solution is completely dechlorinated, maintain the same culture conditions, carry out two subcultures until there is no obvious sediment residue in the culture solution to obtain the complete dechlorination mixed bacteria of tetrachlorethylene.

[0067] ...

Embodiment 2

[0085] Example 2 Isolate Geobacter Geobacter from PCE dechlorination to cis-DCE process

[0086] From figure 1 It can be seen that the complete dechlorination process of PCE is divided into two stages, the latter stage can only be completed by Dehalococcoides, and in the first stage PCE dechlorination speed is faster (0.4mM Cl - / day), and no obvious trans-DCE (partial Dehalococcoides dechlorination product) was observed, which proved that non-Dehalococcoides must participate in the dechlorination of PCE; through community analysis, it was found that there were other PCE dechlorination microorganisms in the mixed bacteria— Geobacter (0.3%). Since Geobacter and Dehalococcoides have similar substrate utilization in the anaerobic dechlorination process, they cannot be effectively separated. In order to obtain the PCE rapid dechlorination microorganism Geobacter at this stage, a new separation method must be designed.

[0087] 1. Separation and purification method

[0088] Firs...

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Abstract

The invention discloses a method for enriching and separating perchloroethylene (PCE) dechlorinating bacteria and application. The invention realizes efficient enrichment culture of PCE complete dechlorinating bacteria, PCE can be completely dechlorinated after 26 days by using the dechlorinating mixed bacteria obtained by the enrichment method, the dechlorinating mixed bacteria show excellent dechlorinating effects, and the abundance of Dehalococoides in the dechlorinating mixed bacteria reaches 68.87%; and efficient PCE dechlorinating bacteria Geobacter is obtained by separation and purification from the enriched dechlorinating mixed bacteria by further using an ecology principle. The method provides favorable technical guidance for realizing efficient enrichment and separation of halogenated organic matter dehalogenation functional microorganisms and obtaining engineering bacterial agents, and has important application value in the technical field of halogenated organic matter anaerobic microorganism remediation.

Description

technical field [0001] The invention belongs to the field of in-situ microbial restoration of halogenated organic pollution, and in particular relates to a method and application for enriching and separating tetrachlorethylene dechlorinating bacteria. Background technique [0002] Perchlorethylene (PCE) is widely used as a solvent in the chemical synthesis process and as a detergent in metal processing and dry cleaning industries because of its good solubility and volatility. When used in production, a large amount of PCE enters the environment due to improper operation and handling, causing serious environmental pollution, especially the soil and groundwater environment. At the same time, PCE is listed as a Class 2A carcinogen because of its strong environmental toxicity and carcinogenic effects on humans. At present, in-situ remediation for PCE contamination mainly includes in-situ physical remediation (such as in-situ thermal remediation), in-situ chemical remediation (h...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34B09C1/10C12R1/01C02F101/36
CPCB09C1/10C02F3/34C02F2101/36C12N1/02C12N1/20
Inventor 汪善全梁咏颐鲁祺鸿
Owner SUN YAT SEN UNIV
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