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Application of myricetin in preparation of drug for treating schistosomiasis

A technology for schistosomiasis and schistosomiasis, which is applied in the field of schistosomiasis japonicum control and biology to achieve the effects of avoiding clinical drug resistance, reducing load, and improving treatment

Active Publication Date: 2020-09-18
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, only dihydromyricetin has been found to have a therapeutic effect on the organ pathological damage caused by schistosomiasis liver fibrosis after schistosomiasis infection, and the role of myricetin in the treatment of schistosomiasis and the liver fibrosis caused by schistosomiasis have therapeutic effects. effect, has not yet been reported

Method used

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  • Application of myricetin in preparation of drug for treating schistosomiasis
  • Application of myricetin in preparation of drug for treating schistosomiasis
  • Application of myricetin in preparation of drug for treating schistosomiasis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The in vitro insecticidal action of embodiment 1 myricetin is detected

[0027] Drug preparation: Myricetin was formulated with DMSO to a final concentration of 10 μM.

[0028] Construction of New Zealand rabbit model infected with Schistosoma japonicum:

[0029] At a room temperature of 25±1°C, place Schistosoma japonicum-positive snails in a 12-well plate, add dechlorinated water to 2 / 3 of the plate, place under an incandescent lamp for 2 hours to escape the cercariae, and shave off the abdominal fur of the animal. Wet the skin with dechlorinated water, stick the coverslip with counted cercariae on the shaved skin, and remove the slide after 20 minutes. New Zealand rabbits are infected with 1000-1200 cercariae per rabbit.

[0030] At the 8th week after infection, New Zealand rabbits were sacrificed by taking blood from the heart and air embolism. After dissection, the adults of Schistosoma japonicum settled in the inferior mesenteric vein and hepatic portal vein wer...

Embodiment 2

[0033] Example 2 Myricetin effectively reduces the number of adult worms in mice infected with Schistosoma japonicum and improves liver fibrosis

[0034] 1. Establishment of a mouse model of Schistosoma japonicum infection

[0035] (1) Balb / c mice were randomly divided into four groups, namely normal control group (ctrl), infection group (infected), praziquantel treatment group (pzq-treated) and myricetin treatment group (me-treated), 8 in each group.

[0036] (2) Except for the normal control group, other mice were infected with 30 ± 2 cercariae of Schistosoma japonicum according to the method described above.

[0037] (3) 36 to 42 days after infection, the praziquantel treatment group and the myricetin treatment group were given intragastric administration of 500 mg / kg praziquantel and 250 mg / kg myricetin (prepared from 0.9% normal saline) to the mice every day. ), and the other two groups were given the same volume of normal saline.

[0038] 2. At the 7th week of Schistoso...

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Abstract

The invention provides application of myricetin in preparation of a drug for treating schistosomiasis. By in-vitro observing an effect of the myricetin for resisting schistosoma japonicum and carryingout in-vivo experiment by establishing a schistosoma japonicum infected mouse model, it is found that the myricetin has good schistosoma japonicum killing effect and an improvement effect on liver fibrosis of schistosoma japonicum infected mice in vivo and in vitro. Moreover, the insecticidal effect of the myricetin is equivalent to that of positive drug praziquantel, the myricetin can replace the application of the positive drug praziquantel to schistosomiasis japonica, possibility is provided for avoiding clinical drug resistance, and the myricetin has a great application prospect.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to the technical field of schistosomiasis prevention and control, and more specifically, to the new application of myricetin in the preparation and treatment of schistosomiasis. Background technique [0002] As a neglected tropical disease, schistosomiasis is prevalent in 78 countries and regions around the world, 779 million people are threatened, conservatively estimated that about 230 million people are infected, and 120 million people are symptomatic, which results in only sub-Saharan Africa. Nearly 300,000 people died from schistosomiasis. Schistosomiasis is a parasitic disease caused by parasites of the genus Schistosoma. There are six types of schistosomiasis-causing worms related to humans, among which Schistosoma mansoni, Schistosoma haematobium and Schistosoma japonicum are the most prevalent. The average lifespan of schistosomiasis in the final host human body is 3 t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P33/12A61P1/16
CPCA61K31/352A61P33/12A61P1/16Y02A50/30
Inventor 吕志跃黄萍成韶芸胡玥周旻昱周洪利马玉斌
Owner SUN YAT SEN UNIV
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