Process method for extracting total cardiac glycoside from Calotropis gigantea

A process method and technology of croissant melon are applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., which can solve the problem of low purity of total cardiac glycosides in croissant melon, improve yield and purity, and achieve separation and extraction. Simple process, simple operation effect

Inactive Publication Date: 2020-09-15
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of process for extracting total cardiac glycosides in the horned melon in order to overcome the defect that the purity of the total cardiac glycosides in the horned melon is not high in the above-mentioned prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] S1: Crush the stems and leaves of croissant melon into coarse powder, pass through a 30-mesh sieve, add 60% ethanol aqueous solution at a weight ratio of 1:1, seal and swell for 1 hour, and put the fully swelled croissant melon coarse powder into reflux In the extractor, add 5 times the amount of 60% ethanol aqueous solution as a solvent to carry out reflux extraction for 1 hour, concentrate the extract, settle, put it into a centrifuge at 1000rpm for centrifugation, and dry the obtained centrifugate to obtain total cardiac glycosides of crocus melon. Through ultraviolet-visible spectrophotometry detection, the mass content of the total cardiac glycosides in the product is 3%, and the recovery rate of the total cardiac glycosides in the product is calculated to be 57.53%.

[0029] S2: After concentrating the extract obtained in step S1, it is absorbed by D101 macroporous resin, and then eluted with 40% ethanol aqueous solution at a flow rate of 2 BV / h for 30 minutes. Aft...

Embodiment 2

[0033] S1: Crush the stems and leaves of croissant melon into coarse powder, pass through a 40-mesh sieve, add 70% ethanol aqueous solution at a weight ratio of 1:1, seal and swell for 1 hour, and put the fully swelled croissant melon coarse powder into reflux In the extractor, add 5 times the amount of 70% ethanol aqueous solution as a solvent to carry out reflux extraction for 1.5h, the number of extractions is twice, the combined extracts are concentrated, settled, put into a centrifuge at 1000rpm for centrifugation, and the obtained centrifuge is dried to produce To obtain the total cardiac glycosides of croissanthus. Detection by ultraviolet-visible spectrophotometry shows that the content of total cardiac glycosides in the product is 4%, and the recovery rate of total cardiac glycosides in the product is calculated to be 76.71%.

[0034] S2: After concentrating the extract obtained in step S1, it is absorbed by D101 macroporous resin, and then eluted with 50% ethanol aqu...

Embodiment 3

[0038] S1: Crush the stems and leaves of croissant melon into coarse powder, pass through a 30-mesh sieve, add 80% ethanol aqueous solution at a weight ratio of 1:1, seal and swell for 1 hour, and put the fully expanded croissant melon coarse powder into reflux In the extractor, add 5 times the amount of 80% ethanol aqueous solution as a solvent and carry out reflux extraction for 2 hours. The number of extractions is three times. After combining the extracts, they are concentrated, settled, put into a centrifuge at 1000rpm for centrifugation, and the obtained centrifuge is dried to obtain ox horns. Total cardiac glycosides of melons. Detection by ultraviolet-visible spectrophotometry shows that the content of total cardiac glycosides in the product is 5%, and the recovery rate of total cardiac glycosides in the product is calculated to be 95.89%.

[0039]S2: After concentrating the extract obtained in step S1, it is absorbed by D101 macroporous resin, and then eluted with 60%...

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Abstract

The invention relates to a process method for extracting total cardiac glycoside from Calotropis gigantea. The process method comprises the following steps: (S1) crushing a Calotropis gigantea raw material into a coarse powder, performing sieving with a 30-40-mesh sieve, mixing the Calotropis gigantea powder with a first ethanol aqueous solution, performing hermetic swelling, and performing refluxextraction on the sufficiently expanded Calotropis gigantea coarse powder to obtain an extracting solution; (S2) concentrating the extracting solution obtained in the step (S1), then performing adsorbing with a D101 macroporous resin, then performing elution with a second ethanol aqueous solution to obtain an elution solution, and concentrating and drying the elution solution to obtain an intermediate crude product; and (S3) loading the intermediate crude product obtained in the step (S2) into a chromatographic column with C18 filler, first carrying out the primary elution by using a first methanol aqueous solution, then carrying out the secondary elution by using a second methanol aqueous solution, collecting two elution solutions, and performing concentrating and drying to obtain the final product. Compared with the prior art, the method has the advantages of low raw material cost, green and environment-friendly extraction process, high yield and purity of the total cardiac glycoside, and the like.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of effective components of natural products, and in particular relates to a process for extracting total cardiac glycosides in ceria melon. Background technique [0002] Cardiac glycosides are a class of steroidal glycosides with cardiotonic effects that exist in nature, also known as cardiac glycosides or cardiac glycosides. Cardiac glycosides have cardiotonic, antitumor, central nervous system, diuretic and molluscicidal activities. It has a wide range of pharmacological activities, and it is mainly used clinically to treat chronic heart failure and certain types of arrhythmias, and it is also effective in anti-tumor. Cardiac glycosides are only distributed in angiosperms, such as Apocynaceae, Scrophulariaceae, Liliaceae, Astragalus, Brassicaceae, Ranunculaceae, etc., and it is complicated and difficult to extract cardiac glycosides from these medicinal materials, mainly becau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/27A61P35/00A61K135/00A61K127/00
CPCA61K36/27A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61P35/00
Inventor 于燕燕郭玲玲胡惠明肖繁花
Owner SHANGHAI INST OF TECH
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