Application of norharmane in improvement of antibiotic antibacterial activity
A technology of methyl harman base and desmethyl harman, which is applied in the application field of desmethyl harman base in improving the antibacterial activity of antibiotics, and achieves the effects of enhancing the antibacterial activity, reducing the bacteriostatic concentration and reducing the occurrence of adverse reactions
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Embodiment 1
[0025] Embodiment 1: Norharmane and antibiotic joint antibacterial action assay
[0026] 1. Materials
[0027] 1.1 Drugs and reagents
[0028] Norharmane, Shanghai Taosu Biochemical Technology Co., Ltd.;
[0029] Polymyxin B, TCI (Shanghai) Chemical Industry Development Co., Ltd.;
[0030] Imipenem-cilastatin sodium, Zhuhai United Laboratories Co., Ltd.;
[0031] Levofloxacin, TCI (Shanghai) Chemical Industry Development Co., Ltd.;
[0032] Ofloxacin, Shanghai Bofeimeike Reagent Co., Ltd.;
[0033] Methanol, Shanghai Lingfeng Chemical Reagent Co., Ltd.;
[0034] Sodium chloride, Shanghai Shisi Hewei Chemical Co., Ltd.;
[0035] Sodium hydroxide, Shanghai Shisi Hewei Chemical Co., Ltd.;
[0036] Peptone, BD difco;
[0037] Yeast powder, BD difco company;
[0038] Agar powder, BD difco company;
[0039] Norharmane mother solution: norharmane was dissolved in methanol to make a 5000 μg / mL mother solution, polymyxin B, imipenem-cilastatin sodium, levofloxacin and ofloxac...
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