Method for collecting fungal spores in deep fungal infection specimen
A technology of deep fungi and fungal spores, applied in the biological field, can solve the problems of low positive rate, poor timeliness, poor fungal antigen and antibody detection specificity, etc., and achieve the effect of improving the positive rate and efficient collection.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0022] like figure 1 As shown, the method for collecting fungal spores in deep fungal infected tissues comprises the following steps:
[0023] (a) Pretreatment: take samples from deeply infected tissues, with a volume of 1×1×2cm, and put the samples into a pre-cooled sterile petri dish; in a biological safety cabinet, use 12cm sterile surgical scissors Crush the tissue and grind it repeatedly in a 2mL glass grinder under ice bath conditions; filter the ground tissue mixture through a 300-mesh nylon mesh, this operation can filter cell membranes and a large number of red blood cells to ensure the collection of prokaryotic or eukaryotic pathogens; After the filtration is completed, use 0.01mol / LPBS solution to wash and filter repeatedly, and transfer to a sterile 20mL centrifuge tube, add 5mL red blood cell lysate (ACKLysisBuffer) to the centrifuge tube, the red blood cell lysate can be removed without damaging the nucleated cells Red blood cells; then centrifuge at 4°C and 150...
Embodiment 2
[0028] like figure 1 As shown, the method for collecting fungal spores in deep fungal infection body fluids comprises the following steps:
[0029] (a) Treatment of body fluids with deep fungal infection: 3 ml of blood was collected, centrifuged at 4000 rpm for 15 min, and the concentrated fungal mixture was collected.
[0030] (b) Fungal culture: the mixture was transferred to a fungal blood culture bottle using a No. 7 cell needle, and conventional fungal culture was performed using a blood culture instrument.
[0031] (c) Collecting fungal spores: Take 5ml of the positive mixture in the fungal blood culture bottle and add it to a 20mL sterile centrifuge tube, centrifuge at 5000rpm for 5min to obtain a precipitate, and then transfer the precipitate to the built-in tube. The fungal spores were collected in a 2 mL sterile EP tube with a pore size of 10 μm biofiltration membrane and centrifuged at 14000 rpm for 10 min at high speed.
Embodiment 3
[0033] like figure 1 As shown, the method for collecting fungal spores from alveolar lavage fluid includes the following steps:
[0034] (a) Treatment of alveolar lavage fluid: take 20 μL of alveolar lavage fluid as a cell smear, and select qualified specimens by microscope observation. The qualified specimens are 2% of total epithelial cells and 2% of red blood cells. The ratio is 6%; filter the qualified specimen through a 300-mesh nylon mesh to remove exfoliated epithelial cells and mucus, and collect all alveolar lavage fluid containing fungi into a 20ml centrifuge tube; centrifuge at 4°C, 1500rpm for 15 minutes, Discard the supernatant and collect the fungus-containing mixture.
[0035] (b) Fungal culture: the mixture was transferred to a fungal blood culture bottle using a No. 7 cell needle, and conventional fungal culture was performed using a blood culture instrument.
[0036] (c) Collect fungal spores: Take 5ml of the mixture in the fungal blood culture bottle and a...
PUM
Property | Measurement | Unit |
---|---|---|
pore size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com