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Lactobacillus rhamnosus and application thereof in inhibition of helicobacter pylori

A technology of Lactobacillus rhamnosus and Helicobacter pylori, applied in the field of microbiology, can solve the problems of unsatisfactory treatment effect, increased drug resistance of Helicobacter pylori, and reduced treatment efficiency

Active Publication Date: 2020-09-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the frequent use of antibiotics in the above-mentioned treatment methods, the drug resistance of Helicobacter pylori is likely to increase, and in the process of using the above-mentioned treatment methods to treat patients, patients often have serious adverse reactions (such as abdominal pain, nausea, diarrhea, etc.), Leading to a reduction in the effective rate of treatment, the treatment effect is often not as expected

Method used

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  • Lactobacillus rhamnosus and application thereof in inhibition of helicobacter pylori
  • Lactobacillus rhamnosus and application thereof in inhibition of helicobacter pylori
  • Lactobacillus rhamnosus and application thereof in inhibition of helicobacter pylori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1: Screening and identification of Lactobacillus rhamnosus

[0073] 1. Screening

[0074] Take fresh feces from healthy people in Kunshan City, Jiangsu Province as the sample. After the sample is pretreated, it is stored in a -80°C refrigerator in about 30% glycerol. Perform gradient dilution with 0.9% normal saline, select the appropriate gradient dilution and spread it on the MRS solid medium, incubate at 37°C for 48 hours, pick typical colonies to streak and purify on the MRS plate, pick a single colony and transfer it to liquid MRS The liquid medium was enriched and stored in 30% glycerol to obtain the strain CCFM1119 (the original serial number of the strain was JS-SZ-2-1).

[0075] 2. Identification

[0076] The genome of CCFM1119 was extracted, and the 16S rDNA of CCFM1119 was amplified and sequenced (completed by Shanghai Sangon Bioengineering Co., Ltd.). After sequencing analysis, the 16S rDNA sequence of the bacterial strain was shown in SEQ ID NO...

Embodiment 2

[0077] Embodiment 2: Inhibitory effect of Lactobacillus rhamnosus on the growth of Helicobacter pylori

[0078] Using MRS liquid medium as a negative control, streak Lactobacillus rhamnosus CCFM1119 on MRS solid medium and culture at 37°C for 48 hours to obtain a single colony; pick a single colony and inoculate it in MRS liquid medium, and inoculate at 37°C Cultivate under the conditions for 18h to activate, and activate two generations continuously to obtain the activation solution; inoculate the activation solution in the MRS liquid medium at an inoculum size of 2% (v / v), and cultivate it for 18 hours at 37°C to obtain the bacterium solution; Bacteria solution was passed through a 0.22 μm sterile filter membrane after being centrifuged at 8000 g for 10 min to obtain a supernatant; the effect of the supernatant on inhibiting the growth of Helicobacter pylori was measured by the Oxford cup method, and the results are shown in Table 1 (the Oxford cup method can refer to the lit...

Embodiment 3

[0082] Example 3: Effect of Lactobacillus rhamnosus on Helicobacter pylori adhesion

[0083] Resuspend Helicobacter pylori cells with F12 to a concentration of 1×10 7 CFU / mL to obtain Helicobacter pylori resuspension; Lactobacillus rhamnosus L.GG cells were resuspended with F12 to a concentration of 1×10 7 CFU / mL to obtain Lactobacillus rhamnosus L.GG resuspension; resuspend Lactobacillus rhamnosus CCFM1119 cells with F12 to a concentration of 1×10 7 CFU / mL to obtain the resuspension of Lactobacillus rhamnosus CCFM1119.

[0084] AGS cells that were not treated with L. rhamnosus L.GG and L. rhamnosus CCFM1119 and not infected with Helicobacter pylori were used as the blank group, and AGS cells that were not treated with L. rhamnosus L.GG and Lactobacillus rhamnosus CCFM1119 Treated Helicobacter pylori-infected AGS cells were used as the model group (Hp group), AGS cells were infected with Helicobacter pylori treated with Lactobacillus rhamnosus L.GG and AGS cells were infecte...

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Abstract

The invention discloses lactobacillus rhamnosus and application thereof in inhibition of helicobacter pylori, and belongs to the technical fields of microorganisms and medicines. The invention provides a lactobacillus rhamnosus strain CCFM1119, and the lactobacillus rhamnosus strain CCFM1119 has the effect of preventing and / or treating helicobacter pylori infection. (1) the lactobacillus rhamnosusstrain CCFM1119 can obviously relieve gastrointestinal symptoms of a patient infected by helicobacter pylori; (2) the lactobacillus rhamnosus strain CCFM1119 can significantly reduce the colonizationamount of helicobacter pylori in the body of a patient infected by helicobacter pylori; and (3) the lactobacillus rhamnosus strain CCFM1119 can significantly improve the removal rate of helicobacterpylori in the body of a patient infected by helicobacter pylori, and thus the lactobacillus rhamnosus strain CCFM1119 has a huge application prospect in the preparation of products (such as foods or medicines) for preventing and / or treating helicobacter pylori infection.

Description

technical field [0001] The invention relates to a strain of Lactobacillus rhamnosus and its application in inhibiting Helicobacter pylori, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] Helicobacter pylori (Hp) is a microaerophilic Gram-negative bacterium that colonizes the gastric mucosa and duodenum. It was first discovered by Marshall and Warren, for which the two scholars won the 2005 Nobel Prize in Physiology or Medicine. Helicobacter pylori is one of the most important epidemic pathogens, and its detection rate in the global population has exceeded 50%. The low level of economic development and poor sanitation are the two main factors for the prevalence of Helicobacter infection. In developed countries, the infection rate of Helicobacter pylori in adults is about 30-40%, and in developing countries, the infection rate of Helicobacter pylori in adults is as high as about 80-90%. [0003] Helicobact...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23L2/38A23L11/00A23C9/123A23L33/00A61K35/747A61P31/04A61P1/04C12R1/225A23L11/50
CPCC12N1/20A23L33/135A23L2/382A23C9/1234A23L33/00A61K35/747A61P31/04A61P1/04A23V2002/00C12R2001/225C12N1/205A23V2400/175A23V2200/3204A23L33/40
Inventor 翟齐啸陈卫赵建新张美怡崔树茂陆文伟田丰伟于雷雷王刚张灏
Owner JIANGNAN UNIV
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