Cholic acid extract and extraction process

A technology for extracting and cholic acid, which is applied in the field of extracting cholic acid from poultry gall, can solve the problems of cumbersome steps, prolonging the production cycle, and inability to remove oil well, achieve low reaction temperature, simplify the production process, and reduce the destructive effect Effect

Inactive Publication Date: 2020-09-01
山东中京生物科技有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the inventor thinks that the steps of the above method are cumbersome, and it cannot remove the grease well, prolonging the production cycle

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cholic acid extract and extraction process
  • Cholic acid extract and extraction process
  • Cholic acid extract and extraction process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Collect fresh duck gallbladder, cut the gall bladder, and collect fresh bile. Take 1000 g of bile and centrifuge at 1450 rpm for 20 min. After the centrifugation is completed, collect the filtrate. Add 10% dilute sulfuric acid under normal temperature conditions, adjust the pH to 3, let it stand for a period of time, and remove the upper layer of grease after the grease is separated.

[0041] Weigh the remaining bile to be 900g, add 800ml of 10% potassium hydroxide to it, keep the temperature of the reactor at 100°C to carry out saponification reaction, and react for 24h. After the saponification reaction is completed, add 10% dilute sulfuric acid again to adjust the pH of the solution to 2, stir slowly until yellow precipitate particles appear in the solution, and then centrifuge to obtain the yellow precipitate part. The yellow particle part is 210 g after weighing, and the yield is 21%.

Embodiment 2

[0043] Collect fresh chicken gallbladder, cut the gall bladder, and collect fresh bile. Take 1000 g of bile and centrifuge at 1480 rpm for 20 min. After the centrifugation is completed, collect the filtrate. Add 8% dilute sulfuric acid under normal temperature conditions, adjust the pH to 4, let it stand for a period of time, and remove the upper layer of grease after the grease is separated.

[0044]Weigh the remaining bile to be 910g, add 1000ml of 8% potassium hydroxide to it, keep the temperature of the reactor at 95°C to carry out the saponification reaction, and react for 20h. After the saponification reaction is completed, add 8% dilute sulfuric acid again to adjust the pH of the solution to 3, stir slowly until yellow precipitated particles appear in the solution, and then centrifuge to obtain a brown precipitated part. After weighing, the brown particle part is 200 g, and the yield is 20%.

Embodiment 3

[0046] Collect fresh duck gallbladder, cut the gall bladder, and collect fresh bile. Take 1000 g of bile and centrifuge at 1400 rpm for 23 min. After the centrifugation is completed, collect the filtrate. Add 12% dilute sulfuric acid under normal temperature conditions, adjust the pH to 3.2, let it stand for a period of time, and remove the upper layer of grease after the grease is separated.

[0047] The remaining bile was weighed to be 920g, 700ml of 12% potassium hydroxide was added thereto, and the temperature of the reactor was kept at 100°C to carry out the saponification reaction for 27h. After the saponification reaction is completed, add 11% dilute sulfuric acid again to adjust the pH of the solution to 2.8, stir slowly until yellow precipitate particles appear in the solution, and then centrifuge to obtain the yellow precipitate part. The yellow particle part is 192g after weighing, and the yield is 19.2%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of cholic acid extraction, and particularly relates to a cholic acid extraction process. The cholic acid is an important medicine production raw material as a processing raw material of cholagogic drugs. At present, most cholic acids are obtained from animal bile, an alkali extraction method is mostly adopted, but cholic acid finished products preparedthrough a traditional process are blocky solids, high in sticky degree and inconvenient to use. The invention provides a novel cholic acid extraction process, which comprises the following steps of: collecting bile; completely removing grease through centrifugation and acidification to reduce the introduction of impurities; and carrying out saponification and acid precipitation to obtain a cholicacid finished product. The cholic acid obtained through the preparation method is in a uniform particle shape; the content of chenodeoxycholic acid is high; subsequent packaging and use are facilitated; and the cholic acid is accepted by vast downstream users.

Description

technical field [0001] The disclosure relates to the technical field of cholic acid extraction technology, in particular to a method for extracting cholic acid from poultry gall gall. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] The herbal records of past dynasties and modern studies have shown that bile of various animals has the effect of clearing away heat, detoxifying and resolving phlegm. Animal medicine is an important part of traditional Chinese medicine, which has the characteristics of abundant resources and definite curative effect. Bile pigment and bile acid are the unique components of bile in animal bile. A large number of pharmacological experiments h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00A61K35/413
CPCA61K35/413C07J9/005
Inventor 丁洪勇秦院生李波付银行
Owner 山东中京生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap