A method and application of poisonous Amanita species identification based on dna micro-barcoding technology

A micro-barcoding and species identification technology, applied in the field of food quality and safety testing, can solve problems such as damage and inability to extract genes

Active Publication Date: 2022-05-03
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Abstract
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Problems solved by technology

But in some processed foods, the intact DNA cannot be extracted due to the damaged DNA ITS Gene, at this time extracted to part ITS Genes (100 bp-400 bp) can also accurately identify species

Method used

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  • A method and application of poisonous Amanita species identification based on dna micro-barcoding technology
  • A method and application of poisonous Amanita species identification based on dna micro-barcoding technology
  • A method and application of poisonous Amanita species identification based on dna micro-barcoding technology

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Embodiment Construction

[0022] The present invention will be further described by way of examples, but the present invention is not limited to the following examples.

[0023] This example is to evaluate the accuracy of DNA micro-barcoding amplification efficiency and identification of toxic Amanita by the following tests.

[0024] 1. Experimental materials

[0025] The names of 43 mushroom samples of 33 species of Amanita used in this study are shown in Table 1. Among them, Amanita pseudoglandum was provided by the Chinese Center for Disease Control and Prevention, and the other samples of Amanita were provided by Professor Chen Zuohong of Hunan Normal University for morphological identification. ITS , Large Subunit Ribosomal LSU ), the Second Largest Subunit of RNA Polymerase II (the Second Largest Subunit of RNA Polymerase II, RPB2 ), β-tubulin ( β-tubulin ) 4 DNA barcodes to identify its species authenticity.

[0026] Table 1 Information of collected Amanita samples

[0027] s...

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Abstract

The invention relates to the technical field of food quality and safety detection, and specifically discloses a DNA micro-barcode-based identification method and application of Amanita species. The method extracts the genomic DNA of the poisonous Amanita, then uses self-designed DNA micro-barcode primers for PCR amplification, sequences the purified PCR products, and compares the sequences of DNA micro-barcode fragments obtained by sequencing with the reference sequences in the public database. The comparison is carried out, and the species identification is carried out according to the matching similarity of the sequences. This method is easy to operate and has high accuracy. It can identify Amanita samples after artificial digestion, and has great application potential in the analysis of Amanita or vomit after accidental ingestion.

Description

technical field [0001] The invention belongs to the technical field of food quality and safety detection, and in particular relates to a method and application for the identification of toxic Amanita species based on DNA micro-barcoding technology. Background technique [0002] Amanita belongs to the kingdom Fungi ( Fungi ), Basidiomycota ( Basidiomycotina ), layer fungi ( Hymenomycetes ), Agaric ( Agaricales ), Amanita family ( Amanitace ), which is the most widely distributed genus of macrofungi. Nearly 500 species of Amanita have been reported and verified worldwide, and more than 130 species (subspecies, varieties and variants) have been reported in my country. Among these amanita species, there are both edible amanita species, such as Amanita caesarea, Amanita caesarea (Amanita caesarea) Amanita ovoidea ), Amanita red and yellow ( Amanita hemibapha ( Amanita virgineoides ), small poisonous rope amanita ( Amanita melleiceps ), Amanita licheniformis ( A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12Q1/04C12N15/11C12R1/645
CPCC12Q1/6895C12Q1/686C12Q2600/156C12Q2563/185
Inventor 陈颖邢冉冉白文明姬庆龙张九凯
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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