A kind of dna molecule and its application and method for obtaining high root mass ramie plants
A DNA molecule, ramie technology, applied in the field of genetic modification, can solve problems such as inconsistent research conclusions
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Embodiment 1
[0039] (1) Acquisition of recombinant Agrobacterium strains containing pBI21-BnWOX8 overexpression vector
[0040] Specific steps are as follows:
[0041] 1). Synthesis of SEQ ID NO.1 sequence:
[0042] It was synthesized by solid-phase phosphoramidite triester method. The sequence of SEQ ID NO.1 is as follows:
[0043] ATTTTATGAATGATCTGTTGATATGTTTTAGACATGTTGATAAGTGGTGCGTTTTTTTCGTTCGATGTGCGTTGTCTTTTTGAGAAACTGCCAAGTTGGGAATTTGATGGCGTAGATATCTGAGACTTTTGTTGCCTGATGTGCTCGTTTCCATGCTTTTGTTGTTTGGGTAATACTTTTGGCATGTTGATATTTGATTGGCTATTGATTTACTAAACAATGTTTTTATTGGGTTTTATTTTGAAAAATTATCCCCAAAGTTTCAAGTTAATGTCAAATGAACATTTTTTATTGACTTTTCTAGACGAGGTGAGGGGTCAGTATAACTTGCGAGTTAGATACAAGATACAAGGGTTTTCTTGATATTGTTTATCCTTTTGGGGCCTAATTTTACGGTTTAGGCCGTATATAATATTTCAAAATTGTCCTTAAAAGCTAAAATAAATTAAATTTTCAATGTTTCTGTGTCTTTATTTTCATTGGTCATTGTTAAGCGAAGATGATTTGGAGATCAGCCGCGAACGTATCGTATCGGTTCGGTTACTAAAAACACCCCTCTTCGTCGGCTTATGGTCTTTGTGTCTCAATTGTCTCTGTCTCAATGTCTCATGTCTCATGTCTCATGTCTCATGTCTCATGTCTCAGTCACTTGTCTCATGTCTCATATT...
Embodiment 2
[0067] In Example 2, the ramie plants obtained by the genetic transformation method using callus as explants were hydrocultured with the ramie variety before modification, and the ramie variety before modification was Zhongzhu No. 2. At the same time, unmodified Zhongzhu No. 2 was selected for water cultivation. Root biomass was measured after 45 days of cultivation.
[0068] The hydroponics method is as follows:
[0069] The ramie plants obtained in the present invention and the common No. 2 ramie plants are topped to remove the top dominance and promote the growth of side branches. When the lateral branch reaches 12cm, it is collected uniformly, trimmed, and cultivated by the disclosed method of ramie water cultivation seedling using the patented ramie factory seedling raising method (CN105052528B). Root biomass was measured after 45 days of cultivation.
[0070] Table 2 Determination of ramie root biomass (dry weight)
[0071]
[0072] Note: * indicates that there is...
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