SARS-CoV-2 neutralizing antibody detection kit

Abstract

The invention relates to an SARS-CoV-2 neutralizing antibody detection kit. The SARS-CoV-2 neutralizing antibody detection kit comprises a solid phase carrier, a first antigen and a second antigen ofS protein of SARS-CoV-2, and the second antigen is marked with a signal substance. Whether a tested person is infected by the new coronavirus or not and whether infection risks exist or not are judgedby detecting a neutralizing antibody through an immunodiagnosis technology, and the method is reliable in theory, practical and feasible and can be completed only in a secondary biosafety laboratory.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a SARS-CoV-2 neutralizing antibody detection kit. Background technique [0002] Coronaviridae is divided into 4 genera α, β, γ and δ, and the α and β genera coronaviruses can infect mammals and humans. Severe acute respiratory syndrome coronavirus 2 (severe acute respiratory syndrome coro-navirus 2, SARS-CoV-2) is a new type of coronavirus belonging to the genus β, round or oval, with a diameter of 60-140nm, and a crown-like shape under an electron microscope. Similar to SARS-CoV that emerged in 2002, SARS-CoV-2 belongs to the subgenus Sarbecovirus (Coronaviridae, Coronaviridae), and both rely on the ACE2 protein on the surface of human cells as a cell entry receptor. Protein sequence analysis found that the amino acid similarity between SARS-CoV-2 and SARS-CoV was 94.6% in seven conserved non-structural proteins. [0003] The existing detection methods for SARS-CoV-2 mainly...

AI Technical Summary

Problems solved by technology

[0005] The detection methods of the new coronavirus mainly include nucleic acid detection and antibody detection. Nucleic acid detection requires high standards for instruments, and the operation is cumbersome, time-consuming, and expensive. Antibody detection methods include magnetic particle chemiluminescence, colloidal gold, and ordinary chemiluminescence. Ordinary chemiluminescence method takes a long time to detect and has low detection efficiency. It mainly relies on pure manual sample addition, which is cumbersome to operate, resulting in large errors in the experimental results; the enzymatic reaction is not thorough enough, and is easily affected by temperature, time and Influenced by external interference factors such as material concentration, low specificity, poor sensitivity, and narrow detection range during detection