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Dental pulp stem cell polymer of exfoliated deciduous teeth and preparation method and application thereof

A technology of dental pulp stem cells and aggregates, applied in the field of biomedicine, can solve the problem of low biological performance of SHED cell aggregates, and achieve the effects of inhibiting cell apoptosis, promoting cell proliferation, and promoting angiogenesis

Pending Publication Date: 2020-08-18
SHENZHEN ZHIBO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are few studies on the biological properties of SHED cell aggregates, and there is a lack of an effective method for cultivating stem cell aggregates using exfoliated deciduous teeth.

Method used

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  • Dental pulp stem cell polymer of exfoliated deciduous teeth and preparation method and application thereof
  • Dental pulp stem cell polymer of exfoliated deciduous teeth and preparation method and application thereof
  • Dental pulp stem cell polymer of exfoliated deciduous teeth and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation, cultivation and identification of dental pulp stem cells from exfoliated deciduous teeth

[0037] 1. Isolation and culture of dental pulp stem cells from exfoliated deciduous teeth

[0038] (1) Obtain deciduous teeth from clinical patients and place them in a centrifuge tube filled with culture fluid.

[0039] (2) In the ultra-clean bench, take out the tooth and place it in a petri dish, rinse the tooth repeatedly with PBS, move the tooth to another petri dish, and take out the pulp tissue.

[0040] (3) Rinse the pulp tissue with PBS repeatedly until the blood on the surface is washed away. Mince the pulp tissue in a petri dish and place it in the digestion solution containing type I collagenase (collagenase I for short) and Dispase under dark conditions, and digest it in a 37°C incubator for 40min-1h, during which time Shake gently every 5-10min until no pulp tissue is formed, then add culture medium to stop digestion. The specific ratio of coll...

Embodiment 2

[0125] Example 2 Initial clinical trial of deciduous tooth pulp stem cell aggregates used in freeze-dried teeth for delayed replantation of completely dislocated young permanent teeth

[0126] 1. Inclusion criteria

[0127] The inclusion criteria of the research objects: children between the ages of 7 and 12 who have completely dislocated the upper anterior teeth due to trauma; the affected teeth are intact but the isolated teeth are kept dry for more than 3 hours (meeting the conditions for delayed replantation); the roots of the affected teeth are not developed Complete and there are deciduous teeth that have not been replaced in the mouth; the patient has no other abnormalities and agrees to sign the informed consent.

[0128] 2. Experimental method and process

[0129] The child, male, 8 years old, completely dislocated teeth 11 and 21 due to trauma 2 days ago, and was kept in a dry place outside the body. Parents signed an informed consent form after fully understanding ...

Embodiment 3

[0145] Example 3 Initial clinical trial of deciduous tooth pulp stem cell aggregates used in freeze-dried teeth for delayed replantation of completely dislocated young permanent teeth:

[0146] The subject of this embodiment is the same as that of the previous embodiment.

[0147] 1. Routine oral examination

[0148] The color of the crown is normal and shiny, and the eruption height of the crown is consistent with that of the contralateral tooth of the same name; percussion pain (1), no cold or heat stimulation pain, and the affected tooth responds to the stimulation during the grinding test at the 6-month follow-up visit; the fixation is removed after one month Teeth still had I degree of looseness during installation, and the mobility was normal during 12-month follow-up consultation.

[0149] 2. Radiological examination

[0150] RVG examination showed that there was no shadow around the root apex, smooth root canal wall and no visible inflammatory absorption area in the ...

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Abstract

The invention discloses a preparation method of a dental pulp stem cell polymer for exfoliating deciduous teeth, the dental pulp stem cell polymer prepared by the preparation method and an applicationof the dental pulp stem cell polymer in preparation of a preparation for permanent tooth reimplantation. The preparation method comprises the following steps: (1) cleaning dental pulp tissue from exfoliated deciduous teeth, (2) digesting the dental pulp tissue by collagenase I and Dispase to obtain a cell suspension, (3) adding a VC culture solution and culturing to obtain a dental pulp stem cellpolymer. The dental pulp stem cells of exfoliated deciduous teeth not only have the characteristics of mesenchymal stem cells, but also express related genes of embryonic stem cells, have higher induced differentiation capacity than the dental pulp stem cells, and have ideal effects on dental pulp regeneration and periodontal healing of completely-dislocated young permanent teeth.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a dental pulp stem cell aggregate of deciduous teeth and its preparation method and application. Background technique [0002] The ultimate ideal goal of tooth regeneration is to replace the lost tooth with a regenerated natural tooth. Stem cells in deciduous teeth are an excellent treatment plan for repairing damaged or diseased tooth tissue, and even for overall restoration after tooth loss. In 2003, the research group of Professor Shi Songtao in the United States isolated stem cells with multi-differentiation ability from the pulp of the crown and root of the exfoliated deciduous teeth for the first time, and named them as stem cells from human exfoliated deciduous teeth, SHED). [0003] Dental pulp stem cells of exfoliated deciduous teeth, dental pulp stem cells (DPSC), and apical papilla stem cells (SCAP) are all odontogenic stem cells and are derived from neural crest mesenchym...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12Q1/6888A61K35/28A61P1/02A61P29/00
CPCC12N5/0664C12Q1/6888A61K35/28A61P1/02A61P29/00C12N2500/38C12N2509/00C12Q2600/158
Inventor 杨涛杨鸣谦
Owner SHENZHEN ZHIBO BIOTECH CO LTD
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