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Lactobacillus crispatus capable of preventing and/or treating helicobacter pylori infection

A technology of Lactobacillus crispatus and Helicobacter pylori, applied in the field of microorganisms, can solve the problems of reduced treatment efficiency, unsatisfactory treatment effect, increased drug resistance of Helicobacter pylori, etc.

Active Publication Date: 2020-08-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the frequent use of antibiotics in the above-mentioned treatment methods, the drug resistance of Helicobacter pylori is likely to increase, and in the process of using the above-mentioned treatment methods to treat patients, patients often have serious adverse reactions (such as abdominal pain, nausea, diarrhea, etc.), Leading to a reduction in the effective rate of treatment, the treatment effect is often not as expected

Method used

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  • Lactobacillus crispatus capable of preventing and/or treating helicobacter pylori infection
  • Lactobacillus crispatus capable of preventing and/or treating helicobacter pylori infection
  • Lactobacillus crispatus capable of preventing and/or treating helicobacter pylori infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1: Screening and Identification of Lactobacillus crispatus

[0073] 1. Screening

[0074] Take fresh feces from healthy people in Kunshan City, Jiangsu Province as the sample. After the sample is pretreated, it is stored in a -80°C refrigerator in about 30% glycerol. Perform gradient dilution with 0.9% normal saline, select the appropriate gradient dilution and spread it on the MRS solid medium, incubate at 37°C for 48 hours, pick typical colonies to streak and purify on the MRS plate, pick a single colony and transfer it to liquid MRS The liquid medium was enriched and stored in 30% glycerol to obtain strain CCFM1118 (the original number of the strain was G14-5M).

[0075] 2. Identification

[0076] The genome of CCFM1118 was extracted, and the 16S rDNA of CCFM1118 was amplified and sequenced (completed by Shanghai Sangon Bioengineering Co., Ltd.). After sequencing analysis, the 16S rDNA sequence of the bacterial strain was shown in SEQ ID NO.1. The sequence ...

Embodiment 2

[0077] Embodiment 2: the cultivation of Lactobacillus crispatus

[0078] After inserting Lactobacillus crispatus CCFM1118 into MRS solid medium and culturing at 37°C for 48 hours, the colonies were observed and observed under a microscope. It was found that the colonies were milky white semicircular protrusions with smooth and moist surfaces and neat edges .

[0079] Lactobacillus crispatus CCFM1118 was inserted into MRS liquid medium and cultured at 37°C for 48 hours. During the cultivation process, the pH of the culture solution was measured by a pH meter at intervals, and it was found that Lactobacillus crispatus CCFM1118 produced acid during the cultivation process.

[0080] Insert Lactobacillus crispatus CCFM1118 into MRS liquid medium and culture at 10-50°C for 48 hours. During the cultivation process, measure the OD of the culture solution with a microplate reader at intervals 600 , it was found that Lactobacillus crispatus CCFM1118 grew best at 30-37°C, and reached a ...

Embodiment 3

[0081] Example 3: Inhibition of Lactobacillus crispatus to Helicobacter pylori

[0082] Using the MRS liquid medium as a negative control, streak Lactobacillus crispatus CCFM1118 on the MRS solid medium and culture it at 37°C for 48 hours to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, and inoculate it at 37°C Cultivate for 18 hours to activate, and activate two generations continuously to obtain the activation solution; inoculate the activation solution in the MRS liquid medium at an inoculum size of 2% (v / v), and cultivate it for 18 hours at 37°C to obtain the bacterial solution; After being centrifuged at 8000g for 10min, pass through a 0.22 μm sterile filter membrane to obtain the supernatant; use the Oxford cup method to measure the effect of the supernatant on inhibiting the growth of Helicobacter pylori, and the results are shown in Table 1 (the Oxford cup method can refer to the literature for details: Zhang Tingting, Zhai Qix...

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Abstract

The invention discloses lactobacillus crispatus capable of preventing and / or treating helicobacter pylori infection, and belongs to the technical field of microorganisms and medicines. The invention provides the lactobacillus crispatus CCFM1118. The lactobacillus crispatus CCFM1118 can inhibit helicobacter pylori, and is specifically characterized in that: (1) the size of an inhibition zone of a lactobacillus crispatus CCFM1118 supernatant on helicobacter pylori can reach 13.14 mm; and (2) the lactobacillus crispatus CCFM1118 can significantly reduce the adhesion of helicobacter pylori to AGScells; therefore, the lactobacillus crispatus CCFM1118 has a huge application prospect in the aspects of inhibiting helicobacter pylori (not aiming at the diagnosis and treatment of diseases) and preparing a helicobacter pylori inhibitor.

Description

technical field [0001] The invention relates to a strain of Lactobacillus crispatus capable of preventing and / or treating Helicobacter pylori infection, and belongs to the technical field of microorganisms and the technical field of medicine. Background technique [0002] Helicobacter pylori (Hp) is an S-shaped or curved Gram-negative bacterium. In 1982, Barry J.Marshall and J.Robin Warren isolated and cultured Helicobacter pylori from human gastric mucosa specimens, thus successfully revealing its potential pathogenic mechanism, and the two won the 2005 Physiological Medicine Award . In 1994, Helicobacter pylori was classified as a class I carcinogen by the World Health Organization. At present, the infection rate of Helicobacter pylori in the world has reached about 50%, and the infection rate of Helicobacter pylori in developing countries is higher than that in developed countries. [0003] Helicobacter pylori infection is a long-term and chronic process. After infecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04A23C9/123A23C11/10A23L33/00A23L2/38C12R1/225A23L11/50A23L11/60
CPCA61K35/747A61P31/04A23C9/1234A23C11/106A23L33/00A23L2/382C12R2001/225C12N1/205A23V2400/133A61K47/6911A61K47/6929A61K47/6925A61K9/20
Inventor 翟齐啸陈卫张美怡于雷雷田丰伟王顺合赵建新张灏
Owner JIANGNAN UNIV
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