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Intracerebral neurochemical substance detection method

A technology of neurochemistry and detection methods, applied in the direction of neural learning methods, neural architecture, material electrochemical variables, etc., can solve the problems of overlapping current responses, indistinguishable and precise quantification, and achieve reduced workload and high flexibility , to solve the effect of mutual interference

Inactive Publication Date: 2020-08-14
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Ultimately, the current responses generated by multiple factors overlap each other, making it difficult to distinguish and quantify accurately

Method used

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  • Intracerebral neurochemical substance detection method
  • Intracerebral neurochemical substance detection method
  • Intracerebral neurochemical substance detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] For the process flow of the detection method of neurochemical substances in the brain, see figure 1 ,details as follows:

[0041] 1. Prepare carbon fiber electrodes according to the preparation method of brain electrochemical detection electrodes provided by the invention patent application with application number CN201911075622.X. The carbon fiber electrode was used as the working electrode, and the Ag / AgCl micro-reference electrode was used as the counter electrode to form a two-electrode system for in vitro voltammetry. The carbon fiber electrode was used as the working electrode, and the Ag / AgCl micro-reference electrode was used as the counter electrode to form a two-electrode system for in vitro voltammetry. Among them, the fast-scan cyclic voltammogram in artificial cerebrospinal fluid is as follows Figure 2A shown. The rapid-scan voltammograms recorded in artificial cerebrospinal fluid standard solutions containing different concentrations of vitamin C after...

Embodiment 2

[0050] The carbon fiber electrode described in Example 1 that has been tested in vitro and successfully constructed a neural network model is used for in situ testing in vivo, and the electrodes are implanted in the SD rat striatum brain region while recording the SD rat striatum in the process of propagating inhibition. Real-time changes in vitamin C, dopamine, and ion concentrations in brain regions. 1 min after the start of recording, local injection of 250 mmol / L KCl solution at a position 1 mm away from the working electrode induced propagation inhibition, and continued recording for 10 min.

[0051] Figure 4A The current raw data extracted from the in vivo test data of the SD rat transmission inhibition model. Among them, the extraction methods of curves a, b, and c are the same as Figure 3A , which respectively reflect the influence of vitamin C, dopamine and ion concentration on the voltammetric curve in the original data. It can be seen from this figure that the ...

Embodiment 3

[0054] The carbon fiber electrode described in Example 1 that has been tested in vitro and successfully constructed a neural network model was used for in-vivo in-situ testing. In order to verify the accuracy of the test results in vivo, electrodes were implanted in the striatal brain region of SD rats and the real-time changes of vitamin C, dopamine and ion concentrations in the striatal brain region of SD rats were recorded during the process of propagating inhibition. One minute after the start of recording, the same amount of artificial cerebrospinal fluid as in Example 2 was locally injected at a position 1 mm away from the working electrode as a blank control experiment, and the recording was continued for 10 minutes.

[0055] Figure 4C It is the original current data extracted from the control experiment data of SD rats. Among them, the extraction methods of curves a, b, and c are the same as Figure 3A, which respectively reflect the influence of vitamin C, dopamine...

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Abstract

The invention provides an intracerebral neurochemical substance detection method which is characterized by comprising the following steps: with a carbon fiber microelectrode as a working electrode, respectively carrying out in-vitro voltammetry tests in different neurochemical substance standard solutions by virtue of a rapid scanning voltammetry method; constructing and training an artificial neural network model or loading an existing artificial neural network model for transfer learning; carrying out in-vivo in-situ analysis on a neurochemical signal by using a rapid scanning voltammetry; extracting a rapid scanning volt-ampere data current value under a characteristic potential according to electrochemical volt-ampere characteristics of a to-be-detected object; normalizing the extracted current value, and constructing a data set; and substituting the data set into the artificial neural network model, and converting a model output result into a concentration calculation value. The intracerebral neurochemical substance detection method provided by the invention can simultaneously and accurately perform quantitative analysis on various substance changes, has good anti-interferenceability, can realize long-time in-vivo in-situ recording, and has the broads application prospects.

Description

technical field [0001] The present invention relates to the field of biochemistry. Specifically, the present invention relates to a method for detecting neurochemical substances in the brain. Background technique [0002] With the development of brain science research, in situ accurate analysis of neurochemical signals in the brain has gradually attracted great attention in the fields of neuroscience and analytical chemistry. Electrochemical analysis methods usually have the advantages of high sensitivity, good selectivity, and high temporal and spatial resolution, and are suitable for in situ analysis in vivo. However, the physiological environment of the living body is complex. On the one hand, the interference of different neurochemical substances is often encountered during the analysis process, resulting in inaccurate concentration analysis of the analyte. On the other hand, many physiological and pathological processes involve the simultaneous changes of multiple neu...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G06N3/04G06N3/08
CPCG01N27/26G01N27/30G06N3/04G06N3/08
Inventor 毛兰群薛亦飞蒋亚楠于萍
Owner INST OF CHEM CHINESE ACAD OF SCI
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