Sulfonamide antibiotic synergistically degraded bacteria and application thereof
A technology of synergistic degradation and antibiotics, applied in the direction of bacteria, microorganisms, microorganisms, etc., to achieve the effect of broad-spectrum detoxification and degradation ability, high degradation efficiency, and great potential for bioremediation
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Embodiment 1
[0042] Isolation and Identification of Degrading Bacteria P27 on One Side of SMX Benzene Ring and Product Degrading Bacteria N27
[0043] 1.1 Isolation and purification of strain P27
[0044] The source of the bacteria was collected from the surplus sludge of a sewage treatment plant in Harbin. 50 mg / L of SMX was added to a 250 mL Erlenmeyer flask (containing 100 mL of sterilized inorganic salt medium) as a mother liquor, and about 5 The mud-water mixture sample of g was cultured on a constant temperature shaker at 30°C with a rotation speed of 150 rpm. After culturing for 7-8 days, prepare the medium in the same way, and transfer 10% of the volume of the enrichment solution to fresh medium. Continuously transferred several times until there were no obvious sludge particles in the enrichment solution and a uniform milky white suspension appeared, then the SMX degradation effect of the enrichment solution was measured. Using 50 mg / L SMX-inorganic salt (SMX-MM) plates, the str...
Embodiment 2
[0050] Analysis of the degradation efficiency of the degrading bacteria P27 on one side of the SMX benzene ring and the degrading bacteria N27 on nitrogen heterocyclic products
[0051] 2.1 Preparation of pure bacteria suspension
[0052] The purified strains were added to the R 2 A solid medium (peptone 0.5 g, yeast extract powder 0.5 g, casein hydrolyzate 0.5 g, glucose 0.5 g, soluble starch 0.5 g, dipotassium hydrogen phosphate 0.3 g, anhydrous magnesium sulfate 0.024 g, sodium pyruvate 0.3 g , agar 15.0 g, distilled water 1000 mL) for activation, picked a single colony to a sterilized inorganic salt medium, and added the corresponding carbon source, and cultured in a shaker at 30°C and 150 rpm. After the bacterial liquid is turbid, transfer it to fresh inorganic medium with 10% transfer amount, repeat the above operation, and the obtained pure bacterial seed liquid with stable degradation ability and growth cycle can be used for subsequent analysis of degradation efficien...
Embodiment 3
[0057] Analysis of Degradation Substrate Spectrum of Sulfa Antibiotics by Strain P27
[0058] Analysis of the substrate spectrum of strain P27: the inoculation source was the pure bacterial suspension prepared in 2.1. In this stage, it was investigated whether the strain P27 also has the ability to degrade the benzene ring side of the nine widely used sulfa antibiotics except SMX, including sulfapyridine, sulfamethazine, sulfamethizole, sulfachloropyridazine, sulfa Dimethoxine, sulfamethoxine, sulfa-5-methoxine, sulfadiazine, and sulfa-metamethoxine. In a 50 mL conical flask (containing 20 mL of sterilized inorganic salt medium), add the corresponding substrate in the form of a mother solution to a final concentration of 50 mg / L, and then add 10% transfer volume to prepare The P27 seed solution of the strain P27 was cultured at 30°C with constant temperature and shaking, and three replicates were set up for each treatment, and the culture medium without bacteria was used as t...
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