Medicine with intervention effect on skin photoaging caused by UVA, and animal model

Abstract

The invention belongs to the technical field of pharmaceutical preparations, and discloses a medicine with an intervention effect on skin photoaging caused by UVA, and an animal model. The medicine isindole-3-methanol, and the maximum inactive dose on HaCaT cells is 10 mu mol / L. HaCaT cells in a logarithmic phase are taken to be inoculated into a 6-hole culture plate according to 2 *105 piece / hole to be cultured in a cell culture box until the cell fusion degree is 70-80%; processing is carried out as follows: a blank control group is covered with a lightproof black paper sheet, the irradiation dose of a UVA model group is 1 J / cm2, an I3C treatment group is treated with 10 [mu] mol / L I3C while UVA irradiating is carried out, and two parallel samples are set for each dose; and the change of the number and morphology of the cells are observed after the irradiation is finished. According to the medicine, the lesions of mouse skin epidermal cells and fiber tissues caused by excessive UVAirradiation can be improved, such that the formation of skin photoaging can be delayed.

Description

Technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and in particular relates to a drug and an animal model for interfering with UVA-induced skin photoaging. Background technique [0002] Human skin aging is a very complicated process, and genetic factors and environmental factors affect it at the same time. At present, it has been found that ultraviolet rays in environmental factors are an important cause of skin aging. Human skin receives ultraviolet radiation from sunlight every day. Studies have shown that long-term ultraviolet radiation will cause skin photoaging and even skin cancer. With the improvement of human living standards and the growth of the aging population, the problem of skin aging has attracted increasing attention. Due to the increasing destruction of the ozone layer in the atmosphere and other factors such as living habits, skin photoaging has also become one of the important health problems that threaten mode...

AI Technical Summary

Problems solved by technology

However, whether I3C can prevent or delay photoaging through this pathway is still unclear

[0008] Through the above analysis, the problems and defects of the existing technology are: the existing technology is mainly used to solve the chemopreventive effect of various tumors, and has not yet realized its role in preventing, delaying and weakening the photoaging effect caused by ultraviolet rays; whether I3C can Protecting cells, reducing damage, or delaying photoaging through anti-oxidative stress and anti-inflammatory responses is not yet clear

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