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Early esophageal cancer combined screening test strip

A technology of esophageal cancer and test strips, which is applied in the field of medicine and biology, can solve the problems of less evaluation, etc., and achieve the effect of increasing the detection rate, reducing mortality, and high detection accuracy

Active Publication Date: 2020-08-07
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The TAAs used in most previous related studies were identified from other types of tumors rather than esophageal cancer, and their sensitivity and specificity in the detection of esophageal cancer still need to be fully evaluated; although some studies Esophageal cancer-associated tumor antigens have been identified using proteomic techniques, but less comprehensive and in-depth evaluation of their elicited autoantibodies as early immunodiagnostic markers for esophageal cancer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: the preparation of test strip

[0058] 1. Experimental materials

[0059] The bioactive raw materials used in the present invention are all commercially available products. Among them, mouse anti-human IgG monoclonal antibody was purchased from abcam company, product number is ab211339; rabbit IgG monoclonal antibody was purchased from abcam company, product number is ab133470; KPNA6 protein was purchased from Invitrogen company, product number is PEP-1150; PDE1A protein was purchased from abcam Company, product number is ab125661; CASK protein was purchased from abcam company, product number is ab131707; goat anti-rabbit IgG monoclonal antibody was purchased from abcam company, product number is ab238531.

[0060] PVC base plate, nitrocellulose membrane, glass fiber membrane, absorbent paper etc. are all existing commercially available products.

[0061] 2. Preparation of binding pads

[0062] (1) Colloidal gold synthesis:

[0063]A colloidal gold sol...

Embodiment 2

[0089] Embodiment 2: the diagnostic value analysis of test strip

[0090] Use the test strip prepared in Example 1 of the present invention to detect the serum samples of pathologically confirmed early esophageal cancer patients and normal people, and evaluate and analyze the value of the test strip of the present invention for early esophageal cancer screening and diagnosis.

[0091] 1. Sample source

[0092] Serum samples from 100 patients with early-stage esophageal squamous cell carcinoma and 100 normal controls were collected from the First Affiliated Hospital of Zhengzhou University. Sera from 100 patients with esophageal cancer (esophageal cancer group) were obtained from newly diagnosed patients who were pathologically diagnosed and did not receive any radiotherapy or chemotherapy. There were 50 males and 50 females, with an average age of 59.1±6.8 years, and the age range was 40 to 80 years; 100 cases were normal Human serum (control group) came from outpatient healt...

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Abstract

The invention belongs to the technical field of medical biology, and discloses an early esophageal cancer combined screening test strip. The early esophageal cancer combined screening test strip comprises a combination pad and a chromatography pad, the combination pad is coated with a mouse anti-human IgG monoclonal antibody and a rabbit IgG monoclonal antibody, and the mouse anti-human IgG monoclonal antibody and the rabbit IgG monoclonal antibody are both provided with detectable markers. Three detection lines and a quality control line are arranged on the chromatography pad, the detection antigens coated by the three detection lines are respectively KPNA6 protein, PDE1A protein and CASK protein, and goat anti-rabbit IgG is coated on the quality control band. According to the test stripdisclosed by the invention, three tumor-associated antigens, namely the KPNA6 protein, PDE1A protein and CASK protein, are combined to serve as markers for screening early esophageal cancer, the teststrip is used for detecting the expression level of autoantibodies of KPNA6, PDE1A and CASK in serum, can effectively detect the esophageal cancer, especially early esophageal cancer, and greatly improves the detection rate of the early esophageal cancer.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a test strip for joint screening of early esophageal cancer. Background technique [0002] Esophageal cancer is one of the most common malignant tumors of the digestive system in humans. The incidence of esophageal cancer ranks eighth among all malignant tumors worldwide, and its case fatality rate ranks sixth among cancer deaths. my country is a country with a high incidence of esophageal cancer in the world, and the tissue type is mainly squamous cell carcinoma (esophageal cancer in the present invention refers to esophageal squamous cell carcinoma, i.e. esophageal squamous cell carcinoma). According to the latest data from the "China Tumor Registration Annual Report" published in 2018, my country's esophageal cancer ranks sixth in the incidence of malignant tumors and fourth in death, posing a serious threat to people's quality of life and family happi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/577G01N33/574G01N33/573G01N33/564G01N33/558G01N33/531
CPCG01N33/57407G01N33/57488G01N33/573G01N33/564G01N33/558G01N2333/916G01N2333/912G01N33/532G01N33/543
Inventor 王立东王盼盼宋昕赵学科杨苗苗程锟徐瑞华靳艳韩文莉蔺红丽李留玉
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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