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Kadsura coccinea peel extract and preparation method and application thereof

A technology of fruit peel extract and tiger skin, applied in the field of natural medicinal chemistry, to achieve the effect of strong inhibitory activity and enhanced inhibitory effect

Active Publication Date: 2020-08-07
HUNAN POLYTECHNIC OF ENVIRONMENT & BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is almost no antiviral research report for the black tiger peel, so whether there is an antiviral composition in the black tiger peel, which viruses its active ingredients can resist, and how to prepare the antiviral composition from the black tiger peel; staff for further research

Method used

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  • Kadsura coccinea peel extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 black tiger peel extract

[0028] Take the fresh peel of Heihuhu, add ethanol with a volume fraction of 80% according to the ratio of material to liquid: 1g: 25mL, conduct ultrasonic extraction at 60°C for 35min (the power of ultrasonic extraction is 180W), and concentrate the extract under reduced pressure to obtain the extract. The extract is dried to obtain the ethanol extract of black tiger peel, that is, the black tiger peel extract.

Embodiment 2

[0029] The preparation of embodiment 2 black tiger peel extract

[0030] (1) Take the fresh pericarp of Heihuhuo, add ethanol with a volume fraction of 80% according to the ratio of material to liquid: 1g: 25mL, conduct ultrasonic extraction at 60°C for 35min (the power of ultrasonic extraction is 180W), and concentrate the extract under reduced pressure to obtain Extract, and then dry the extract to obtain the alcoholic extract of black tiger peel;

[0031] (2) put the black tiger peel ethanol extract on the silica gel column (the silica gel column is filled with 200-300 mesh silica gel; the weight consumption of silica gel is 30 times of the black tiger peel ethanol extract weight), first use the volume of 5 times the column volume A mixed solvent composed of chloroform and methanol with a ratio of 90:10 was used to elute and remove impurities; and then a mixed solvent composed of chloroform and methanol with a volume ratio of 5 times the column volume was used for elution, ...

Embodiment 3

[0036] Embodiment 3 black tiger peel extract antiviral activity test

[0037] Test method: After MDCK cells grow to a monolayer in a 96-well cell culture plate, wash with Eagle’s solution and discard the supernatant, and then add 100 μL of 100TCID to each well 50 Then add 100 μL of the drug solution diluted with MEM to each well, and place it at a temperature of 37°C and 5% CO 2 and incubating in an incubator with saturated humidity for 48 hours, then remove the old solution in the 96-well plate, and add 10 μL of CCK8 solution to each well; after incubating in the incubator for 1 hour, measure the absorbance at 450 nm with a microplate reader. Simultaneously set the cell control group and the virus control group, and calculate the IC 50 value.

[0038] The test viruses are Coxsackie virus B3 (VR30 strain) and herpes simplex virus type I (SM44 strain);

[0039] The test drug is the black tiger peel extract prepared in Examples 1 and 2 and Comparative Example 1.

[0040] IC ...

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Abstract

The invention relates to the technical field of natural medicinal chemistry, and particularly discloses kadsura coccinea fruit peel extract and a preparation method and application thereof. The preparation method of the kadsura coccinea fruit peel extract comprises the following steps: taking kadsura coccinea fruit peel, adding ethanol, carrying out ultrasonic extraction at 50-70 DEG C, and concentrating and drying the extracting solution to obtain the kadsura coccinea fruit peel alcohol extract, namely the kadsura coccinea fruit peel extract. The kadsura coccinea fruit peel extract prepared by the method shows certain antiviral activity.

Description

technical field [0001] The invention relates to the technical field of natural medicine chemistry, in particular to a black tiger peel extract and its preparation method and application. Background technique [0002] Black Tiger (Kadsura coccinea) is a perennial evergreen climbing vine of the genus Schisandra in the family Schisandraceae, also known as Lengfantuan, Guoshanlong, Bufuna, Drilling Wind, etc. It is widely distributed in Hunan, Guizhou, Fujian, Yunnan and other places. Black tiger is a kind of edible and medicinal wild fruit with high development potential. The rhizome is commonly known as Daxueteng, which has high medicinal value and is an important Chinese medicinal material. The fruit is large, purple in color, and is an aggregated berry , sweet and sour taste, can be eaten raw, rich in amino acids, mineral elements and polyphenols. At present, there are many studies on the chemical components, pharmacological effects, and volatile oil components of the rhizo...

Claims

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Application Information

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IPC IPC(8): A61K36/79A61K36/57A61P31/22A61P31/14A61K131/00
CPCA61K36/79A61K36/57A61P31/22A61P31/14A61K2236/333A61K2236/51A61K2236/55Y02A50/30
Inventor 贺灵芝梁忠厚
Owner HUNAN POLYTECHNIC OF ENVIRONMENT & BIOLOGY
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