Method for calculating metabolic levels of different brain regions after spinal cord injury by <13>C labeled glucose

A technology for spinal cord injury and glucose, applied in diagnostic recording/measurement, medical science, measuring devices, etc., can solve the problems of inapplicability, low sensitivity, and failure to give metabolic pathways, etc., and achieve high accuracy and simple calculation methods Effect

Pending Publication Date: 2020-08-07
GENERAL HOSPITAL OF NINGXIA MEDICAL UNIV
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Problems solved by technology

However, MRS cannot provide relevant information on metabolic pathways and metabolic intervals, and is not suitable for samples with low concentrations, and its sensitivity is not high

Method used

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  • Method for calculating metabolic levels of different brain regions after spinal cord injury by &lt;13&gt;C labeled glucose
  • Method for calculating metabolic levels of different brain regions after spinal cord injury by &lt;13&gt;C labeled glucose
  • Method for calculating metabolic levels of different brain regions after spinal cord injury by &lt;13&gt;C labeled glucose

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Embodiment 1

[0054] 13 C-labeled glucose calculates the cortex, subcortical tissue neurotransmitter, energy metabolite concentration and metabolic abundance after rat T10 spinal cord injury, and the method includes the following steps:

[0055] Allen percussion apparatus was used to complete the rat model of spinal cord injury at T10 level. On the 3rd day after injury, 4.0% to 5.0% isoflurane mixed with air was anesthetized on the day of the experiment, and 1.5% to 2.5% isoflurane was used to maintain the anesthesia. The caudal vein was cannulated with PE50 tubing, and the infusion line was connected to the swivel and suspended in the center of the cage to avoid entanglement of the line during the movement of the rat. The other end of the rotation is connected to the infusion pump with a PE50 tube, and injected through the tail vein at a variable speed [1- 13 C] Glucose for 20 minutes. Rats were then sacrificed by head-focused microwave irradiation. After microwave treatment, blood samp...

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Abstract

The invention discloses a method for calculating metabolic levels of different brain regions after spinal cord injury by <13>C labeled glucose. The method comprises the following steps: (a), manufacturing a spinal cord injury model by adopting different methods and instruments according to experimental requirements; (b), catheterizing the caudal vein of the rat, injecting [1-<13>C] glucose, killing the animal by a microwave radiation method, and separating a target brain region; (c), preparing a target brain area NMR sample, and collecting NMR original data; (d), performing normalization processing on the original data; (e), performing metabolite concentration calculation; and (f), calculating metabolic abundance. Therefore, the technical problems that isotope markers are accumulated andcannot continue to participate in the metabolic process, related information of metabolic pathways and metabolic intervals can not be provided and the sensitivity is low in the prior art are solved, and the method for calculating the metabolic concentration and metabolic abundance of the cerebral cortex substances after spinal cord injury is simple and high in result obtaining accuracy.

Description

technical field [0001] The invention belongs to the technical application field of stable isotope labeling glucose, in particular to a 13 C-labeled glucose method for calculating metabolic levels in different brain regions after spinal cord injury. Background technique [0002] The traditional method to study brain metabolism after spinal cord injury is a biochemical method using 14C or 18F labeled 2-deoxyglucose (2-DG) as a tracer. In recent years, 13 C NMR methods can give more detailed information about metabolic pathways and metabolic intervals in the study of brain metabolism. Labeled 2-deoxyglucose (glucose analogue) can be transported from capillaries to cells like glucose, and phosphorylated in the cytoplasm to generate the intermediate product 2-deoxyglucose-6-phosphate (2-DG-P) . Labeled 2-DG-P cannot be further metabolized, accumulates in cells, and can be detected by corresponding autoradiography (detection of 14C labeling) and positron electron tomography (P...

Claims

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Application Information

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IPC IPC(8): A61B5/00A61B5/055G01N24/08G01R33/465
CPCA61B5/055A61B5/4866A61B2503/40A61B2503/42G01N24/08G01R33/465
Inventor 夏鹤春王杰吴亮牛占锋
Owner GENERAL HOSPITAL OF NINGXIA MEDICAL UNIV
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