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Electronic detection device and kit for influenza viruses and rhinoviruses

An influenza virus, electronic detection technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problem of time-consuming and labor-intensive virus separation methods, and achieve the effect of low-cost detection and convenient operation.

Pending Publication Date: 2020-07-31
石家庄洹众生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The virus isolation method is time-consuming and labor-intensive, and the enzyme immunoassay and nucleic acid sequence analysis methods require specialized equipment, professional personnel and infrastructure, so they have certain limitations in effective clinical treatment

Method used

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  • Electronic detection device and kit for influenza viruses and rhinoviruses
  • Electronic detection device and kit for influenza viruses and rhinoviruses
  • Electronic detection device and kit for influenza viruses and rhinoviruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Such as figure 1 and figure 2 As shown, the present embodiment provides an electronic detection device for influenza virus and rhinovirus, comprising: a photoelectric detection module, a data processing module and a colloidal gold detection test paper; the colloidal gold test paper comprises a substrate, a nitrocellulose membrane 7, Gold pad 6 and sample pad 3; the nitrocellulose membrane 7 is fixed on the substrate; the gold pad 6 is arranged between the nitrocellulose membrane 7 and the sample pad 3; the gold pad One side of 6 overlaps with the part of the nitrocellulose membrane 7; the other side of the gold pad 6 overlaps with the part of the sample pad 3; the nitrocellulose membrane 7 is coated with quality control line 9 and detection line; the quality control line 9 is located on the side away from the gold pad 6; the detection line includes multiple virus detection lines; the gold pad 6 is made of a mixture of multiple gold-labeled virus monoclonal antibodies;...

Embodiment 2

[0046] The present invention also provides an electronic detection kit for influenza virus and rhinovirus, the electronic detection kit for influenza virus and rhinovirus includes: the electronic detection device for influenza virus and rhinovirus as described in Embodiment 1, such as image 3 The sample collection tube 10, the nasopharyngeal swab 14 and the lysate 13 are shown; in addition, the electronic detection kit also includes instructions.

[0047] Such as Figure 4 As shown, the nasopharyngeal swab 14 is used to collect samples to be tested. The nasopharyngeal swab 14 is a disposable sampling swab, the length of the nasopharyngeal swab 14 is 15cm, and the sampling head of the nasopharyngeal swab 14 is a nylon flocking sampling head 15. There is a broken depression 16 near the sampling head 4cm, and the nasopharyngeal swab 14 is sterilized with ethylene oxide.

[0048] The lysis solution 13 is contained in the sample collection tube 10, and the lysis solution 13 is u...

Embodiment 3

[0053] This embodiment provides a specific electronic detection device for influenza virus and rhinovirus.

[0054] The electronic detection device for influenza virus and rhinovirus comprises an electronic detection device housing 1, a photoelectric detection module, a data processing module and colloidal gold detection test paper. The housing 1 of the electronic detection device also includes a detection cap 2 , a sample pad 3 , and a liquid crystal display 4 . The sample pad 3 is long and thin, with a length of 4 cm, which is convenient to use the sample collection tube 10 provided in the kit for detection and sample addition.

[0055] The photoelectric detection module contains four LED light sources (light-emitting diodes) and photoelectric sensors. The four LED light sources form a parallel arrangement of light-emitting diode arrays and respectively correspond to a quality control line 9 and three detection lines on the colloidal gold test paper. Its function is The col...

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Abstract

The invention relates to an electronic detection device and kit for influenza viruses and rhinoviruses and relates to the field of respiratory virus detection. The electronic detection device comprises a photoelectric detection module, a data processing module and colloidal gold detection test paper, wherein the colloidal gold test paper comprises a bottom lining, a nitrocellulose film, a gold padand a sample pad, the nitrocellulose film is fixed on the bottom lining, the gold pad is arranged between the nitrocellulose film and the sample pad, one side of the gold pad is partially overlappedwith the nitrocellulose film, the other side of the gold pad is partially overlapped with the sample pad, the nitrocellulose film coats a detection line and a quality control line close to one side ofthe gold pad, the detection line comprises a plurality of virus detection lines, the gold pad is made of mixture of multiple gold-labeled virus monoclonal antibodies, the photoelectric detection module is used for detecting color changes of the quality control line and the detection line, and the data processing module is used for judging whether influenza virus or rhinovirus infection exists ornot according to the color change detected by the photoelectric detection module. The electronic detection device is advantaged in that purposes of rapidness, convenient operation and low-cost detection can be achieved.

Description

technical field [0001] The invention relates to the technical field of respiratory virus detection, in particular to an electronic detection device and kit for influenza virus and rhinovirus. Background technique [0002] Respiratory viruses refer to a large class of viruses that invade the respiratory tract and cause local lesions of the respiratory tract or only use the respiratory tract as an invasion portal, mainly causing lesions of tissues and organs outside the respiratory tract. According to statistics, more than 90% of acute respiratory infections are caused by such viruses. Especially for children, respiratory viruses are important pathogens of acute respiratory infections in infants and young children, common ones include influenza virus (Influenza Virus, Flu), rhinovirus (Rhinovirus, RhV), respiratory syncytial virus (Respiratory Syncytial Virus, RSV) )Wait. [0003] Influenza is caused by influenza virus, which has strong infectivity, fast transmission, short ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/56983G01N33/558G01N2333/095G01N2333/11
Inventor 张少丹田颖
Owner 石家庄洹众生物科技有限公司
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