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Biological valve material based on EDC/ NHS activation and recombinant human collagen modification and preparation method thereof

A human-derived collagen and biological valve technology, applied in the field of biological valve materials and their preparation, can solve the problems of poor anticoagulation performance, anticalcification performance and poor biocompatibility, and achieve the enhancement of cell adhesion and anticoagulation, Facilitates endothelialization and reduces immunogenicity

Active Publication Date: 2020-07-31
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned problems in the prior art, the present invention provides a biological valve material based on EDC / NHS activation and recombinant human collagen modification and its preparation method, which can effectively solve the problems of anticoagulant performance, Problems of anti-calcification performance and poor biocompatibility

Method used

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  • Biological valve material based on EDC/ NHS activation and recombinant human collagen modification and preparation method thereof
  • Biological valve material based on EDC/ NHS activation and recombinant human collagen modification and preparation method thereof

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Embodiment 1

[0026] A method for preparing a biological valve material modified based on EDC / NHS activation and recombinant human collagen, comprising the following steps:

[0027] (1) Collect fresh porcine pericardium, place in distilled water, shake and wash at 4°C, 100r / min for 2h;

[0028] (2) Soak the pig pericardium after cleaning in step (1) in an aqueous glutaraldehyde solution with a volume concentration of 0.625% for 24 hours to obtain an animal-derived pericardium biomaterial crosslinked by glutaraldehyde;

[0029] (3) After cleaning the glutaraldehyde-crosslinked animal-derived pericardial biological material, soak it in 50mmol / L MES buffer solution (pH=5.3) containing EDC, soak for 30min, and then add NHS to a final concentration of 50mmol / L , after soaking for 5 hours, take it out and wash;

[0030] (4) Soak the biological material obtained in step (3) in a PBS solution of 30 mg / ml recombinant human type III collagen for 24 hours, then take it out and clean it with distilled...

Embodiment 2

[0033] A method for preparing a biological valve material modified based on EDC / NHS activation and recombinant human collagen, comprising the following steps:

[0034] (1) Collect fresh porcine pericardium, place in distilled water, shake and wash at 4°C, 100r / min for 2h;

[0035] (2) Soak the pig pericardium after cleaning in step (1) in an aqueous glutaraldehyde solution with a volume concentration of 0.625% for 24 hours to obtain an animal-derived pericardium biomaterial crosslinked by glutaraldehyde;

[0036] (3) Wash the animal-derived pericardial biological material cross-linked by glutaraldehyde and soak it in 500mmol / L MES buffer (pH=5.3) containing EDC for 15min, then add NHS to a final concentration of 500mmol / L, After soaking for 3 hours, take out and wash;

[0037] (4) Soak the biological material obtained in step (3) in a PBS solution of 15 mg / ml recombinant human type III collagen for 24 hours, then take it out and clean it with distilled water; wherein, the rec...

Embodiment 3

[0040] A method for preparing a biological valve material modified based on EDC / NHS activation and recombinant human collagen, comprising the following steps:

[0041] (1) Collect fresh porcine pericardium, place in distilled water, shake and wash at 4°C, 100r / min for 2h;

[0042] (2) Soak the pig pericardium after cleaning in step (1) in an aqueous glutaraldehyde solution with a volume concentration of 0.625% for 24 hours to obtain an animal-derived pericardium biomaterial crosslinked by glutaraldehyde;

[0043] (3) Wash the animal-derived pericardial biological material cross-linked by glutaraldehyde, soak it in 500mmol / L MES buffer solution (pH=5.3) containing EDC, soak for 10min, and then add NHS, the final concentration is 500mmol / L , after soaking for 2 hours, take out and wash;

[0044] (4) Soak the biological material obtained in step (3) in a PBS solution of 5 mg / ml recombinant human type III collagen for 24 hours, then take it out and clean it with distilled water; ...

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Abstract

The invention provides a biological valve material based on EDC / NHS activation and recombinant human collagen modification and a preparation method thereof. The preparation method comprises the following steps: (1) soaking a biological valve material crosslinked by glutaraldehyde in an EDC / NHS solution, and then cleaning; and (2) soaking the cleaned biological valve material obtained in the step(1) in a recombinant human collagen solution, and then cleaning to obtain the biological valve material. The biological valve material provided by the invention can effectively solve the problems ofpoor anticoagulation performance, calcification resistance and biocompatibility of existing biological valve materials.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a biological valve material based on EDC / NHS activation and recombinant human collagen modification and a preparation method thereof. Background technique [0002] Heart valve disease is a common disease of valve degeneration, which is anatomically manifested by narrowing of blood pathways or valve insufficiency. Treatment of heart valve disease includes open heart valve replacement surgery and percutaneous heart valve replacement surgery. Thoracotomy is traumatic, high-risk, slow recovery, and requires extracorporeal circulation support, which is unacceptable to many patients. Percutaneous heart valve replacement surgery has become the main trend of valve surgery in the future because of its small trauma and low risk to patients. [0003] Biological heart valves refer to a class of biomedical materials used to replace human diseased heart valves. Biologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/24A61L27/50
CPCA61L27/24A61L27/3625A61L27/3641A61L27/3687A61L27/3691A61L27/50A61L2300/42A61L2430/20A61L2430/40
Inventor 王云兵杨立郑城雷洋张兴栋
Owner SICHUAN UNIV
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