Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for determining four benzodiazepine drugs in blood

A benzodiazepine and blood technology, applied in the field of drug detection, can solve the problems of poor accuracy and many interfering substances, and achieve the effects of simple operation, reducing the influence of interfering substances and eliminating matrix effects.

Inactive Publication Date: 2020-07-28
NINGBO MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problems of many interfering substances and poor accuracy in the traditional detection method of benzodiazepines, the present invention provides a method for measuring four kinds of benzodiazepines in blood with high specificity, effectively reducing the influence of interfering substances and high accuracy. The Azepine Approach

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining four benzodiazepine drugs in blood
  • Method for determining four benzodiazepine drugs in blood
  • Method for determining four benzodiazepine drugs in blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A method for measuring 4 kinds of benzodiazepines in blood, comprising the steps of:

[0047] (1) Take 1ml of plasma sample in a 5mL polypropylene centrifuge tube, add 2mL of formic acid / acetonitrile (0.5:99.5, V / V) to shake and extract for 5min, centrifuge at a centrifugal force of 3000g for 5min, absorb the supernatant to obtain the extract;

[0048] (2) Take 1.0mL extract and transfer to another 5mL polypropylene centrifuge tube, then add 5mg of composite adsorbent, then add 500mg of dewatering agent, vortex for 5min, centrifuge at 3000g for 5min, absorb the supernatant, Obtain purification liquid; Composite adsorbent is the mixture of magnetic graphene-based polyamide and C18, referred to as PAA@Mag-G+C18; In the composite adsorbent, the mass ratio of magnetic graphene-based polyamide and C18 is 3: 1; The dehydrating agent is a mixture of anhydrous magnesium sulfate and anhydrous sodium sulfate; the mass ratio of anhydrous magnesium sulfate and anhydrous sodium sulf...

Embodiment 2

[0069] A method for measuring 4 kinds of benzodiazepines in blood, comprising the steps of:

[0070](1) Take 1ml of plasma sample in a 5mL polypropylene centrifuge tube, add 3mL of formic acid / acetonitrile (0.5:99.5, V / V) to shake and extract for 5min, centrifuge at a centrifugal force of 3000g for 5min, absorb the supernatant to obtain the extract;

[0071] (2) Transfer 1.0 mL of the extract to another 5 mL polypropylene centrifuge tube, then add 10 mg of composite adsorbent, then add 500 mg of water remover, vortex for 5 min, centrifuge at 3000 g for 5 min, absorb the supernatant, Obtain purification liquid; Composite adsorbent is the mixture of magnetic graphene-based polyamide and C18, referred to as PAA@Mag-G+C18; In the composite adsorbent, the mass ratio of magnetic graphene-based polyamide and C18 is 4: 1; The dehydrating agent is a mixture of anhydrous magnesium sulfate and anhydrous sodium sulfate; the mass ratio of anhydrous magnesium sulfate and anhydrous sodium su...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
collision energyaaaaaaaaaa
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of drug detection, in particular to a method for determining four benzodiazepine drugs in blood. The method comprises the following steps of: (1) adding anextraction solvent into a plasma sample for extraction to obtain an extracting solution; (2) adding a composite adsorbent and a water removal agent into the extracting solution, and carrying out purification and water removal treatment to obtain a purified solution; and (3) filtering the purified liquid, and performing LC-MS / MS on-machine detection and analysis. The composite adsorbent is used for purifying the extracting solution, so that residual impurities in the plasma sample are removed, the influence of interferents is reduced, and a matrix effect can be basically and completely eliminated; the water removal agent is added to remove moisture in the plasma sample and reduce the influence of a matrix on measurement; two pairs of ions are respectively used for quantification and qualitative analysis, so that the specificity of a detected object is ensured, the influence of the interferents is reduced. The method is simple, convenient and rapid to operate, high in flux, low in costand capable of effectively detecting the levels of four benzodiazepine drugs in the body of a clinically poisoned patient.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a method for measuring four kinds of benzodiazepines in blood. Background technique [0002] Sedative drugs are commonly used clinical drugs, which can inhibit the central nervous system and are mainly used for insomnia, anxiety, tension, fear, epilepsy and preoperative sedation. At present, the abuse of sedative drugs is relatively high, and the use is tending to be younger. More and more young people are dependent on such drugs, and even use them illegally. Adverse events caused by this are not uncommon. Therefore, it is of great practical significance to carry out the detection research of sedative drugs. [0003] Benzodiazepines are one of the sedative drugs. Most benzodiazepines are derivatives of 1,4-benzodiazepines. There are more than 20 commonly used clinically. Although they are similar in structure, the anxiolytic, sedative-hypnotic, anticonvulsant, muscle rel...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/08G01N30/14G01N30/72C08K3/04C08G73/02C08L79/02
CPCG01N30/02G01N30/06G01N30/08G01N30/14G01N30/72C08K3/042C08G73/028G01N2030/062C08K2201/01
Inventor 金米聪陈晓红林梦张昱周健俞建成蔡美强
Owner NINGBO MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products