Method for determining four benzodiazepine drugs in blood
A benzodiazepine and blood technology, applied in the field of drug detection, can solve the problems of poor accuracy and many interfering substances, and achieve the effects of simple operation, reducing the influence of interfering substances and eliminating matrix effects.
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Embodiment 1
[0046] A method for measuring 4 kinds of benzodiazepines in blood, comprising the steps of:
[0047] (1) Take 1ml of plasma sample in a 5mL polypropylene centrifuge tube, add 2mL of formic acid / acetonitrile (0.5:99.5, V / V) to shake and extract for 5min, centrifuge at a centrifugal force of 3000g for 5min, absorb the supernatant to obtain the extract;
[0048] (2) Take 1.0mL extract and transfer to another 5mL polypropylene centrifuge tube, then add 5mg of composite adsorbent, then add 500mg of dewatering agent, vortex for 5min, centrifuge at 3000g for 5min, absorb the supernatant, Obtain purification liquid; Composite adsorbent is the mixture of magnetic graphene-based polyamide and C18, referred to as PAA@Mag-G+C18; In the composite adsorbent, the mass ratio of magnetic graphene-based polyamide and C18 is 3: 1; The dehydrating agent is a mixture of anhydrous magnesium sulfate and anhydrous sodium sulfate; the mass ratio of anhydrous magnesium sulfate and anhydrous sodium sulf...
Embodiment 2
[0069] A method for measuring 4 kinds of benzodiazepines in blood, comprising the steps of:
[0070](1) Take 1ml of plasma sample in a 5mL polypropylene centrifuge tube, add 3mL of formic acid / acetonitrile (0.5:99.5, V / V) to shake and extract for 5min, centrifuge at a centrifugal force of 3000g for 5min, absorb the supernatant to obtain the extract;
[0071] (2) Transfer 1.0 mL of the extract to another 5 mL polypropylene centrifuge tube, then add 10 mg of composite adsorbent, then add 500 mg of water remover, vortex for 5 min, centrifuge at 3000 g for 5 min, absorb the supernatant, Obtain purification liquid; Composite adsorbent is the mixture of magnetic graphene-based polyamide and C18, referred to as PAA@Mag-G+C18; In the composite adsorbent, the mass ratio of magnetic graphene-based polyamide and C18 is 4: 1; The dehydrating agent is a mixture of anhydrous magnesium sulfate and anhydrous sodium sulfate; the mass ratio of anhydrous magnesium sulfate and anhydrous sodium su...
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