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Application of reagent for detecting and targeting biomarker in oral squamous cell carcinoma

A reagent and oral technology, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, and medical preparations containing active ingredients, etc. It can solve the problems of no significant increase in survival rate, poor prognosis, and restricted surgical scope.

Inactive Publication Date: 2020-07-28
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the treatment methods are constantly improving, the scope of surgery is severely limited because of the close contact with important tissues and organs during oral cavity surgery.
In addition, there are abundant blood vessels and nerves in the neck and facial tissues, the incidence of cervical lymph node metastasis and invasion is high, and the prognosis is poor. In recent years, the 5-year survival rate has not increased significantly (about 50%-60%)

Method used

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  • Application of reagent for detecting and targeting biomarker in oral squamous cell carcinoma
  • Application of reagent for detecting and targeting biomarker in oral squamous cell carcinoma
  • Application of reagent for detecting and targeting biomarker in oral squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1 QPCR detects the differential expression of lncRNA

[0069] 1. Collect 33 cases of surrounding normal mucosa tissue and oral squamous cell carcinoma tissue, all of which were confirmed by pathological diagnosis, and all patients did not receive any form of treatment before operation. Surgical excised samples were frozen in liquid nitrogen.

[0070] 2. RNA extraction

[0071] Take out the tissue samples frozen in liquid nitrogen, put the tissue samples into a pre-cooled mortar for grinding, and extract and isolate RNA according to the instructions in the kit. details as follows:

[0072] 1) Add Trizol and place at room temperature for 5 minutes;

[0073] 2) Add 0.2ml of chloroform, vibrate the centrifuge tube vigorously, mix well, and place it at room temperature for 5-10min;

[0074] 3) Centrifuge at 12000rpm for 15min, transfer the upper aqueous phase to another new centrifuge tube (be careful not to absorb the protein material between the two aqueous ...

Embodiment 2

[0099] Example 2 Silencing detection and functional verification of lncRNA

[0100] 1. Cell culture

[0101] Human oral squamous cell carcinoma SCC-15 cells stored in liquid nitrogen were taken out and inoculated in DMEM medium at 37°C, 5% CO 2 Cells were cultured in a constant temperature incubator. After 24 hours, the cells showed adherent growth and recovered. The medium was successfully changed every 1-2 days, digested with trypsin and made into a cell suspension for experiments.

[0102] 2. Cell transfection

[0103] Cells are divided into 2×10 5 Inoculate / well into six-well cell culture plates at 37°C, 5% CO 2 cultured in an incubator. Cells in the logarithmic phase of proliferation (about 80%) were discarded, washed twice with PBS, added 2 ml of DMEM and starved for 1 h in an incubator, using liposome transfection reagent 2000 (purchased from Invitrogen) For transfection, the specific operation was carried out according to the instructions. The experiment was div...

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PUM

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Abstract

The invention discloses application of a reagent for detecting and targeting a biomarker in oral squamous cell carcinoma. The biomarker is lncRNA LINC01679. The invention discloses application of theLINC01679 in preparation of a product for diagnosing the oral squamous cell carcinoma and the product for diagnosing the oral squamous cell carcinoma. Meanwhile, the invention discloses application ofthe LINC01679 in preparation of a pharmaceutical composition for treating the oral squamous cell carcinoma and the pharmaceutical composition for treating the oral squamous cell carcinoma.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of reagents for detecting and targeting biomarkers in oral squamous cell carcinoma. Background technique [0002] Oral squamous cell carcinoma (OSCC) is a metastatic malignancy of epithelial origin and the eleventh most common cancer worldwide (Hussein AA, Helder MN, deVisscher JG, et al. Incidence of oral and oropharynx cancer in patients younger than 45years versus older patients: A systematic review [J]. EurJCancer2017; 82:115-127.). About 600,000 new cases are added every year, and it is also the fifteenth most common cause of death from cancer in the world (Candia J, Fernandez A, Somarriva C, et al. Deaths due to oral cancer in Chile in the period 2002-2012[ J]. Rev Med Chil. 2018; 146(4): 487-493.). In recent years, the global incidence of oral squamous cell carcinoma has shown a significant increase trend, and the age of onset is gradually getting younger, but the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/00A61K31/7088A61P35/00
CPCA61K31/7088A61K45/00A61P35/00C12Q1/6886C12Q2600/136C12Q2600/158
Inventor 杨承刚肖枫
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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