Experimental method for researching anti-articular cartilage cell apoptosis of resveratrol

Abstract

The invention discloses an experimental method for researching anti-articular cartilage cell apoptosis of resveratrol. The ability of resveratrol to inhibit articular cartilage cell apoptosis is verified by establishing crowd research, in vivo experiments and in vitro experiments, and the mechanism of action of resveratrol to inhibit articular cartilage cell apoptosis is discussed to verify the 'effect axis hypothesis' of resveratrol-p38 MAPK signal pathway down-regulation-anti-apoptosis, which proves that the potential action targets of resveratrol in the screened p38 MAPK pathway have a targeting relationship, and further defines the overall regulation effect of resveratrol on a plurality of targets in the pathway. Experiments prove that resveratrol has the ability to inhibit articular cartilage cell apoptosis, and provides experimental basis for clinical practice of resveratrol in treating osteoarthritis.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an experimental method for studying resveratrol's resistance to articular chondrocyte apoptosis. Background technique [0002] Osteoarthritis (OA) is a complex disease that can affect the entire joint. With the acceleration of the aging of the social population, more and more middle-aged and elderly people are troubled by OA; [0003] Epidemiological research results show that: in 2014, the prevalence of knee OA in Sweden was 15.4%-25.4%; the incidence of hip OA in American male residents was 23.8%-32.2%; The prevalence of OA is 44.5% and 0.8% respectively; the high prevalence of OA directly shows that OA has become one of the important public health problems worldwide; OA patients mainly present symptoms such as joint pain, swelling, and mobility impairment , not only seriously affects the quality of life of patients, but also brings a huge economic burden to the family and...

AI Technical Summary

Problems solved by technology

[0004] The current clinical practice shows that the effect of improving the clinical symptoms of OA patients is not satisfactory, and OA treatment is still a worldwide problem. It is particularly important to improve the quality of life of OA patients due to the clinical symptoms of OA patients; however, the clinical treatment of osteoarthritis is mainly to treat the symptoms, and there are few effective drugs for "curing the root cause". Large trauma and other limitations make it difficult to promote on a large scale; multiple studies have confirmed that oxidative damage and a significant decrease in the number of chondrocytes are common in the cartilage microenvironment of OA patients, and the former is one of the main inducing factors for the latter; therefore, how to effectively reduce The level of oxygen free radicals in chondrocytes is one of the ways to delay and prevent the apoptosis of articular chondrocytes; according to the previous experimental results and the latest research progress, the project team believes that the good antioxidant properties of resveratrol can reduce the risk of osteoarthritis. Apoptosis of chondrocytes and improvement of clinical symptoms bring new hope

[0005] Among the currently discovered drugs that can be used for the treatment of osteoarthritis, resveratrol (Resveratrol, RES) is a non-flavonoid polyphenol natural compound with great application prospects, and its biological effects are shown in the figure , resveratrol can eliminate wind and dampness, dissipate blood stasis and relieve pain, relieve cough and reduce phlegm, and is suitable for the treatment of degenerative bone diseases such as osteoarthritis. During the occurrence of osteoarthritis, oxygen free radicals can inhibit cartilage Cell proliferation, promote excessive apoptosis and necrosis of chondrocytes, destroy the normal structure of joints, and damage articular cartilage. Modern pharmacological experiments show that resveratrol can obviously scavenge free radicals and has good antioxidant properties, but The effect and mechanism of articular chondrocyte apoptosis are still rarely reported; the results show that resveratrol can enhance the activity of human C28 / I2 articular chondrocytes and promote cell proliferation; however, how does resveratrol specifically exert Anti-apoptotic effect of articular chondrocytes? If this key "black box" mystery is not revealed, the clinical practice of resveratrol in the treatment of osteoarthritis will be limited, greatly hindering the development of osteoarthritis treatment technology

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