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Application of mulberry twig flavone and diphenyl ethylene extract in preparing medicament for inhibiting uric acid reabsorption transporter

A technology of stilbene and mulberry flavonoids, which is applied in the directions of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problems of not finding the quality of ingredients, monitoring, etc., and achieves clear regulation effect, clear action target, and good safety. Effect

Inactive Publication Date: 2009-08-12
ZHEJIANG CONBA PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Sangzhi has a good clinical basis, it has not been able to find appropriate ingredients for quality control

Method used

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  • Application of mulberry twig flavone and diphenyl ethylene extract in preparing medicament for inhibiting uric acid reabsorption transporter
  • Application of mulberry twig flavone and diphenyl ethylene extract in preparing medicament for inhibiting uric acid reabsorption transporter
  • Application of mulberry twig flavone and diphenyl ethylene extract in preparing medicament for inhibiting uric acid reabsorption transporter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of Mori flavonoids and stilbenes extracts:

[0026] (1) Take dry mulberry branches, heat reflux three times, add 7 times of 60% ethanol each time, 2 hours each time, combine the extracts, filter, and concentrate the filtrate under reduced pressure to contain 0.5g of medicinal materials per ml of liquid medicine, let it cool , filter, and discard the dregs.

[0027] (2) Take the filtrate, and elute with distilled water of 2-6 times the volume of the column bed at a flow rate of 1-4BV / h on a pretreated HPD-600 macroporous adsorption resin column, with a flow rate of 1--4BV / h, discard the water eluent, and then elute with 60% ethanol with 2-6 times the volume of the column bed at a speed of 1-4BV / h, collect the eluent, recycle the ethanol and concentrate to a thick paste, and depressurize Dry to get dry extract.

[0028] (3) Taking mosperiside as a reference substance, the weight ratio content of stilbenes in the mulberry flavonoids and stilbenes extract is ...

Embodiment 2

[0031] Inhibition and regulation of uric acid reabsorption transporters by flavonoids and stilbenes extracts of Morus alba:

[0032]Oxonic acid potassium salt is a uricase inhibitor, which can induce and inhibit the decomposition of uric acid, increase the serum uric acid level in the body, and cause animal models of hyperuricemia. It is widely used in the world to evaluate the anti-hyperuricemia and gout effects of drugs. The present invention also uses hyperuricemia model animals to verify the uric acid-lowering curative effect of the drug, and the curative effect is reliable and remarkable.

specific Embodiment approach

[0033] 1. Experimental animals: Kunming mice, male, weighing 24±2g.

[0034] 2. Drug preparation: Mori flavonoids, stilbene extract (ER), and allopurinol are dispersed in normal saline for intragastric administration. The dosages are 300mg / kg for the low dose and 600mg for the high dose. / kg, Apu=5mg / kg.

[0035] 3. Experimental materials: HPD-600 macroporous adsorption resin, Trizol Reagment (Invigen), chloroform, isopropanol, absolute ethanol, DEPC, M-MLV reverse transcriptase, PCR kit, RIPA lysate, uric acid reagent kit, creatinine kit.

[0036] 4. Experimental equipment: high-speed refrigerated centrifuge, high-speed homogenizer, Bio-radio-rad vertical electrophoresis tank, MBI-PCR instrument, horizontal electrophoresis tank, etc.

[0037] 5. Experimental model: hyperuricemia model

[0038] 6. Establishment of the model: After one week of adaptation to the environment, the animals were given oxonic acid potassium salt 250 mg / kg by intragastric administration; the contro...

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Abstract

The invention discloses components of flavonoids of mulberry branches and extracts of stilbenes and a new purpose thereof. Animal test results show that the flavonoids of mulberry branches and the extracts of stilbenes have exact inhibition effect for a uric acid reabsorption transporter with abnormally increased animal expression of a hyperuricemia model, but no significant inhibition effect for the expression of the uric acid reabsorption transporter at the normal level of a normal animal, thereby having good safety. The flavonoids of mulberry branches and the extracts of stilbenes can be prepared into a drug for inhibiting the uric acid reabsorption transporter together with the related pharmaceutical excipients by the conventional preparation method, and a pharmaceutical composition for inhibiting the uric acid reabsorption transporter prepared by taking the compound as an active ingredient can be used for treating the diseases which are related to the abnormal high expression of the uric acid reabsorption transporter.

Description

technical field [0001] The present invention relates to mulberry flavonoids and stilbenes extracts, in particular to the components of mulberry flavonoids and stilbenes extracts and their new application in the preparation of drugs for inhibiting uric acid reabsorption transporters, and using the compounds as active ingredients for the preparation of pharmaceutical compositions for inhibiting uric acid reabsorption transporters. Background technique: [0002] In recent years, a large number of epidemiological studies at home and abroad have shown that the prevalence of hyperuricemia / gout is increasing year by year. Hyperuricemia not only induces gouty arthritis, urolithiasis, and uric acid nephropathy, but also increases blood uric acid levels. Hyperuricemia is closely related to abnormal lipid metabolism, obesity and other diseases. Hyperuricemia / gout has become an important disease that threatens human health. Decreased excretion and / or increased production of uric acid i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/605A61P19/06A61K135/00
Inventor 孔令东胡季强王彩萍胡林水黄正标张宪郑珺王星
Owner ZHEJIANG CONBA PHARMA
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