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Application of rutoside in preparation of osteosarcoma treating drug

A treatment drug and osteosarcoma technology, applied in the preparation of osteosarcoma treatment drugs, the field of rutin, can solve problems such as orthopedic diseases or tumor treatment effects that have not yet been found

Inactive Publication Date: 2016-06-01
刘勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies in the prior art have found that rutin has definite antitussive, antiasthma and antibacterial effects, but it has not been found to have a therapeutic effect on orthopedic diseases or tumors

Method used

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  • Application of rutoside in preparation of osteosarcoma treating drug
  • Application of rutoside in preparation of osteosarcoma treating drug
  • Application of rutoside in preparation of osteosarcoma treating drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 (in vivo inhibition experiment of rutin to osteosarcoma cells)

[0028] 1 test material

[0029] 1.1 Experimental animals

[0030] SPF grade experimental mice were purchased from the market.

[0031] 1.2 Experimental osteosarcoma cells

[0032] The osteosarcoma cells used in the experiment include: fibroblastic osteosarcoma; sclerosing osteosarcoma; osteolytic osteosarcoma; mesenchymal chondrosarcoma; juxtacortical chondrosarcoma;

[0033] 1.3 Test samples

[0034] Pure rutin, sealed and stored at 4°C. When in use, prepare a solution of the required concentration with distilled water at about 35-40°C for intragastric administration to animals, and store in a refrigerator at 4°C. The drug can be used within 7 days after preparation.

[0035] 1.4 Drugs and reagents

[0036] Positive control drug: cyclophosphamide for injection, powder injection in ampoule, 0.2g / bottle. When in use, it is prepared with physiological saline to make a medicinal solution of...

Embodiment 2

[0051]Embodiment 2 (in vitro inhibition experiment of rutin to osteosarcoma cells)

[0052] 1 Experimental materials

[0053] 1.1 Samples to be tested

[0054] Pure rutin, packed in a sealed plastic box, stored in a refrigerator at 4°C. When used, it is prepared with 1640 culture medium containing 10% calf serum and diluted in multiples for use in cell experiments, and it is freshly prepared when used. The period of use after the preparation of the medicine is 3 to 4 days.

[0055] 1.2 Osteosarcoma cell lines

[0056] Osteosarcoma cells include: fibroblastic osteosarcoma; sclerosing osteosarcoma; osteolytic osteosarcoma; mesenchymal chondrosarcoma; juxtacortical chondrosarcoma; In the 1640 culture medium of serum, passaging once every 2 days, and the cells in the logarithmic growth phase were taken for experiments.

[0057] 1.3 Instruments and reagents

[0058] Reagent:

[0059] Improved RPMI1640 culture medium; mycoplasma-free newborn bovine serum; MTT solution; dimeth...

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Abstract

The invention provides application of rutoside in preparation of an osteosarcoma treating drug. Through experimental means, it is found that rutoside not only has the pharmacological actions such as the oxidation resistance and lipid metabolism regulation which are disclosed in the prior art, but also has the outstanding osteosarcoma inhibiting effect. In-vivo experiments find that proliferation of entity osteosarcoma can be significantly inhibited after rutoside is taken orally or injected, in-vitro experiments find that the growth speed of osteosarcoma cells is obviously decreased in a cell culture system containing rutoside, and therefore the pharmacological property of rutoside on inhibiting the osteosarcoma can be verified. In addition, it is found that rutoside is wide in treatment range and has the exact inhibiting effect on the fibroblast type, the sclerotic type, and bone limphosarcoma and the like. On the basis of the novel pharmacological property of rutoside, the novel application of preparing the osteosarcoma treating drug from rutoside is determined, the treatment effect of the drug is exact, the osteosarcoma inhibiting effect is obvious, and therefore the outstanding popularization prospect is achieved.

Description

technical field [0001] The invention relates to the technical field of drugs for treating orthopedic diseases, in particular to the application of rutin for preparing drugs for treating osteosarcoma. Background technique [0002] Osteosarcoma is the most common type of malignant bone tumor, which develops from mesenchymal cell lines. The rapid growth of the tumor is due to the direct or indirect formation of tumor bone-like tissue and bone tissue through the cartilage stage. Under the action of external factors (such as viruses), the load-bearing bones of the lower limbs cause cell mutations, which may be related to the formation of osteosarcoma. The typical osteosarcoma originates in the bone, and another completely different type is the osteosarcoma juxtaposed with the bone cortex, which originates from the periosteum and nearby connective tissue. The latter is less common and has a better prognosis. [0003] Osteosarcoma is still a disease with high mortality from malig...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P35/00
CPCA61K31/7048
Inventor 刘勇
Owner 刘勇
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