3D bioprinting ink and its preparation method and application

A bioprinting and 3D printing technology, applied in the field of 3D printing, can solve problems such as the reduction of silk fibroin content, the adverse effects of chemical reagents on the long-term growth of cells, and the inability to fully demonstrate the biological characteristics of silk fibroin materials, etc., to achieve high mechanical properties , optimize the rheological properties, improve the effect of printability

Active Publication Date: 2022-05-24
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, although the methods currently used by researchers to add chemical reagents or blend other high-viscosity biomaterials can significantly improve the printability of silk fibroin materials, the content of silk fibroin in bioinks prepared by this method often decreases. , failed to fully demonstrate the excellent biological characteristics of silk fibroin materials, and the residue of chemical reagents would have adverse effects on the long-term growth behavior of cells

Method used

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  • 3D bioprinting ink and its preparation method and application
  • 3D bioprinting ink and its preparation method and application
  • 3D bioprinting ink and its preparation method and application

Examples

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Embodiment 1

[0050] In this embodiment, a method for preparing a 3D bioprinting ink for 3D bioprinting a bone defect scaffold is provided, and the method includes the following steps:

[0051] Step 1, prepare silk fibroin aqueous solution;

[0052] Silk fibroin aqueous solution is obtained after degumming, dissolving, dialysis and centrifugation of silk. The degumming, dissolving and dialysis processes all adopt common technical means in the field. For details, please refer to the references Rockwood D N, Preda R C, Yücel, Tuna, et al. Materials fabrication from Bombyx mori silk fibroin[J].Nature Protocols,2011,6(10) : 1612-1631.. Wherein, the molecular weight cut-off of the dialysis bag used in the dialysis process is 3500kDa. The concentration of the final prepared silk fibroin aqueous solution was 10 wt%.

[0053] Step 2, the silk fibroin aqueous solution is made into a silk fibroin electrogel under the action of a stable electric field;

[0054] The silk fibroin aqueous solution pr...

Embodiment 2

[0059] In this example, the pure silk fibroin 3D bioprinting ink 3D printing gel scaffold prepared by Example 1 is provided.

[0060] Put the pure silk fibroin 3D bioprinting ink prepared in Example 1 on the 3D printer, open the 3D printer control panel, set the temperature of the nozzle to 5°C, and place the pure silk fibroin 3D bioprinting ink on the nozzle for temperature pre-control for 15 minutes. , so that the pure silk fibroin 3D bioprinting ink reaches the preset nozzle temperature. Use the platform to receive the print holder with the platform temperature set to 5°C. 3D print pure silk fibroin gel scaffolds according to the model set by the 3D printer, see 3D printed pure silk fibroin gel scaffolds Figure 5 , the number of layers is 10.

Embodiment 3

[0062] This embodiment provides the application of a pure silk fibroin 3D printing scaffold in 3D bioprinting bone defect materials, including:

[0063] The pure silk fibroin gel scaffold prepared in Example 2 was frozen at -20°C for four to five hours, and then placed in a freezer at -80°C for overnight freeze-drying to obtain pure silk fibroin 3D printing. Freeze-dried scaffolds.

[0064] Scanning electron microscopy (SEM) was used to observe the 3D printed scaffolds prepared above. The freeze-dried scaffolds had micro-scale microporous structures. For details, please refer to the structure. Image 6 .

[0065] The 3D printed scaffolds prepared above were used for in vitro cell culture, and the cultured cells were pre-osteoblasts MC3T3, and the cultured cells were labeled with fluorescent proteins. Figure 7 It is a confocal image of the 7th day of cell culture. It can be seen from the figure that a lot of fluorescence is displayed inside and on the surface of the scaffold...

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Abstract

The invention relates to a 3D bioprinting ink, a preparation method and application thereof. The preparation method of the 3D bioprinting ink comprises the following steps: applying a constant voltage of less than 50V to the silk fibroin aqueous solution, and the voltage application time is less than 120min, to obtain the silk fibroin electrogel, and then removing the silk fibroin at 30°C to 100°C The air bubbles in the electrogel, after cooling, give the 3D bioprinting ink. The bioprinting ink of the present invention is suitable for 3D printing technology. It is prepared by using pure silk fibroin without adding other chemical reagents. The obtained pure silk fibroin ink has both good 3D printability and excellent biocompatibility, and The cross-linking method after printing is simple, and the mechanical properties of the cross-linked scaffold are good.

Description

technical field [0001] The invention relates to the technical field of 3D printing, in particular to a 3D bioprinting ink and a preparation method and application thereof. Background technique [0002] Three-dimensional (3D) printing technology is an emerging manufacturing technology developed based on the principle of layered manufacturing. It forms 3D structures in a layer-by-layer manner through computer control. Biological 3D printing technology is a branch of 3D printing technology. 3D bioprinting is a comprehensive discipline that combines computer science, cell biology, materials science and medicine. Bio-3D printing ink, referred to as bio-ink, is a key component of bio-3D printing. The ideal bio-ink should have good printability, and should also have many aspects such as biocompatibility, degradability, toxicity and side effects, and suitable mechanical properties. Due to the high requirements for bioprinting inks, the current bioinks suitable for different biopri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09D11/30C09D11/04A61L27/22A61L27/50A61L27/52B29C64/112B33Y10/00B33Y70/00
CPCC09D11/30C09D11/04A61L27/227A61L27/50A61L27/52B29C64/112B33Y10/00B33Y70/00A61L2430/02C08L89/00
Inventor 王卉陈晓敏张克勤
Owner SUZHOU UNIV
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