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A kind of microbe-induced mineralization reinforcement soil grouting method by introducing urease inhibitor

A urease inhibitor and microorganism technology, applied in the field of soil reinforcement, can solve the problems of uneven curing effect, increase the difficulty and cost of construction, and blockage of calcium carbonate deposition, so as to reduce the local deposition rate, delay the hydrolysis of urea and carbonic acid. Formation of calcium, effect of retarding deposition

Active Publication Date: 2022-03-18
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of the reasons that limits the large-scale application of this curing technology is that in order to achieve a better curing effect, the cement must be injected continuously at a low concentration and at a low rate, which will undoubtedly increase the difficulty and cost of construction
In addition, due to the relatively rapid reaction process, it is easy to react at the part where the cementing liquid first contacts the bacterial liquid to form a large amount of calcium carbonate deposition, resulting in local blockage, so that the cementing liquid cannot continue to penetrate into a wider soil body to complete curing, resulting in overall failure. Uneven curing effect

Method used

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  • A kind of microbe-induced mineralization reinforcement soil grouting method by introducing urease inhibitor

Examples

Experimental program
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Effect test

example 1

[0039] (1) Weigh 20g of yeast extract, 10g of NH4Cl, 10mg of MnSO4·H2O and 24mg of NiCl·6H2O, dissolve in 1000ml of deionized water, configure the required liquid medium, and adjust the pH to 8.5 with 1M NaOH solution. Sterilize the liquid medium at a high temperature of 121°C for 45 minutes, return the sterilized medium to room temperature, inoculate the Bacillus pasteurianus into the medium, and culture it with shaking at a temperature of 30°C at a frequency of 150r / min, culture time 24h.

[0040] (2) The sample preparation mold adopts a pvc pipe with an inner diameter of 38mm and a height of 100mm, and the sand soil adopts Xiamen ISO standard sand, with an average particle size of D 50 = 0.72 mm. The height of the sand filling is about 80mm, and permeable stones are laid at both ends of the sand column. The initial porosity of the sample is about 0.38 measured by the drainage volume. Water is injected into the sand column and the excess air is saturated with negative pres...

example 2

[0044] The preparation method of this example is the same as that of Example 1, except that the dosage of additive NBPT is increased, and the content of NBPT used accounts for 0.1% of the total amount of urea in the cementing liquid.

[0045] The test results show that after adding 0.1% of the additive NBPT, while simplifying the grouting process, the curing strength can reach 2.71MPa, and the grouting opening will not be quickly blocked in the early stage of grouting, and the overall curing effect can be more uniform.

example 3

[0047] The preparation method of this example is the same as that of Example 1, except that the dosage of additive NBPT is increased, and the content of NBPT used accounts for 0.5% of the total amount of urea in the cementing liquid.

[0048] The test results show that after adding 0.5% of the additive NBPT, while simplifying the grouting process, the curing strength can reach 1.45MPa, and the grouting opening will not be quickly blocked in the early stage of grouting, and the overall curing effect can be more uniform.

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Abstract

The invention belongs to the technical field of soil reinforcement, and discloses a soil grouting method by introducing a urease inhibitor to induce mineralization and reinforcement of soil. In this method, the urease-producing bacteria liquid is fully mixed with the soil to be solidified, and left to stand until the attachment of the urease-producing bacteria liquid on the particle surface of the soil to be solidified is completed; then, the urease inhibitor is added to the cementing liquid, fully stirred and dissolved, and the Grouting; wherein, the cementing liquid is a mixed solution of calcium chloride and urea, and the urease inhibitor is a competitive inhibitor of urease, and the dosage of the urease inhibitor is far lower than the urea mass content in the cementing liquid, so as not to affect the final result of urea hydrolysis. allow. The invention uses the competitive inhibitor of urease to compete with urea for the active site of urease, reduces the efficiency of urease to decompose urea, thereby effectively delaying the process of urea hydrolysis at the initial stage of grouting, reducing local blockage at the initial stage of grouting, simplifying the grouting process, and uniform curing effect.

Description

technical field [0001] The invention belongs to the technical field of soil reinforcement, and relates to a soil grouting method for soil reinforcement induced by urease inhibitors, and more specifically, relates to a novel foundation treatment method for improving soil strength by using microorganisms to induce mineralization. It can improve the uniformity of curing effect while simplifying the grouting process. Background technique [0002] The method of microbially induced calcium carbonate generation and solidified soil (MICP) is a new type of ground treatment technology. It is the CO produced by the metabolism of certain microorganisms in the soil 2 In alkaline environment and Ca in cementing fluid 2+ Combined to form calcium carbonate deposits, it plays a role of filling and cementation between soil particles, so as to achieve the purpose of solidifying the soil. It can be seen from its working principle that compared with traditional chemical grouting and cement gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): E02D3/12C12N1/20C12R1/07
CPCE02D3/12C12N1/20
Inventor 徐望清郑俊杰崔明娟宋杨吴超传杨丽颖
Owner HUAZHONG UNIV OF SCI & TECH
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