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Preparation method and application of bacteria-enzyme synergistic fermented rice bran meal for improving growth performance of growing and fattening pigs

A performance bacteria enzyme and collaborative fermentation technology, applied in biochemical equipment and methods, microorganism-based methods, bacteria used in food preparation, etc., to achieve the effect of improving nutrient digestibility and enhancing serum antioxidant capacity

Pending Publication Date: 2020-07-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are currently domestic fermentations of rice bran meal with a single bacterium or enzyme, but it is mostly used to extract small peptides or soluble dietary fiber from rice bran meal, improve its nutritional value and apply it to fattening pigs. Reports are still very rare

Method used

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  • Preparation method and application of bacteria-enzyme synergistic fermented rice bran meal for improving growth performance of growing and fattening pigs
  • Preparation method and application of bacteria-enzyme synergistic fermented rice bran meal for improving growth performance of growing and fattening pigs
  • Preparation method and application of bacteria-enzyme synergistic fermented rice bran meal for improving growth performance of growing and fattening pigs

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Experimental program
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Effect test

Embodiment 1

[0022] A preparation method of bacteria-enzyme synergistic two-step fermentation feed, the specific preparation method is as follows:

[0023] (1) Preparation of activation medium

[0024] Prepare strain activation medium, according to the mass ratio:

[0025] Bacillus subtilis activation medium contains 5-10% peptone, 1-5% yeast extract, 5-10% sodium chloride; Saccharomyces cerevisiae activation medium contains 5-10% peptone, 1-5% Yeast extract, 5-10% molasses; Lactobacillus plantarum activation medium contains 5-10% peptone, 1-5% yeast extract, 1-2% molasses, 0.5-1% tomato powder, 0.1- 0.5% potassium dihydrogen phosphate.

[0026] (2) Strain activation and preparation of fermented seed solution

[0027] Insert 0.1-0.5% of Bacillus subtilis ZJUAF-3 and Saccharomyces cerevisiae into the respective activation medium in step (1), and incubate with oxygen at 37°C for 12 hours. Lactobacillus plantarum ZJUAF-5 bacteria powder was inserted into the activation medium in step (1) ...

Embodiment 210

[0035] Embodiment 2 replaces the fattening pig feed of 8% rice bran and 2% soybean hulls with 10% fermented feed

[0036] The formula (by mass ratio) of the fattening pig ration after the improvement of the present invention is:

[0037] 10% fermented feed, 70% corn, 15% soybean meal, 2% soybean oil, 0.65% calcium hydrogen phosphate, 0.3% salt, 0.55% stone powder, 1.5% premix, fully mixed in the mixer Evenly, the fattening pig feed that promptly obtains hair feed content is 10%.

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Abstract

The invention discloses a preparation method and application of bacteria-enzyme synergistic fermented rice bran meal for improving growth performance of growing and fattening pigs. The method comprises: (1) preparing a strain activation culture medium; (2) strain activation and fermentation seed liquid preparation: inoculating bacillus subtilis, saccharomyces cerevisiae and lactobacillus plantaruminto the activation culture medium for activating, performing mixing, adding acid protease and phytase, and performing uniform mixing to obtain a fermentation seed liquid; and (3) a two-step fermentation process: premixing rice bran meal and a soybean hull raw material in a ratio of 4:1, adding the fermentation seed liquid obtained in the step (2) according to 5-10% of the premixed raw materials,introducing warm water to make the total moisture account for 35-40% of the total mass of the fermentation system, performing uniform stirring, transferring obtained mixture into a fermentation tank,performing fermentation for 24 hours, filling an anaerobic breathing bag, and performing fermentation for 48 hours to obtain a fermented feed. The fermented feed replaces protein raw materials in daily ration of the fattening pigs with 10% of equal nitrogen, the feed intake and daily gain of the fattening pigs can be remarkably improved, the feed conversion ratio is reduced, the nutrient digestibility of the fattening pigs is improved, and the serum oxidation resistance of the fattening pigs is enhanced.

Description

technical field [0001] The invention belongs to the technical field of animal feed, and in particular relates to a method for preparing fermented feed and a method for improving the growth performance of fattening pigs by using the same. Background technique [0002] In the face of new situations such as African swine fever and the comprehensive ban on antibiotics, how to respond to the development of efficient and safe biological feed through feed nutrition, improve the conversion efficiency of grain and non-grain raw materials to animal husbandry products and feed utilization, and reduce the use of antibiotics is an important issue for the feed industry. Direction of development. [0003] my country is the world's largest producer and consumer of rice. In 2017, my country's rice production and consumption were 146 million tons and 141 million tons respectively. Rice bran meal is a by-product obtained after oil extraction of rice bran produced by rice production. It account...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/18A23K10/37A23K50/30C12N1/20C12R1/125
CPCA23K10/12A23K10/18A23K10/37A23K50/30C12N1/20A23V2400/169Y02P60/87
Inventor 汪以真苏维发路则庆郝丽红王成张煜王凤芹
Owner ZHEJIANG UNIV
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