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Fermentation material for composite microorganism and preparation method thereof

A technology of compound microorganisms and fermented materials, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low enzyme production effect and total number of viable bacteria, unable to guarantee feed intake and feeding speed, etc. Achieving the effect of high feed nutrition

Inactive Publication Date: 2008-05-28
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this technology, the bacteria have not undergone any selection and induction, and the enzyme production effect and the total number of viable bacteria are very low. At the same time, a simple amino acid formula is added without any chemical or biological reaction process. food intake and food intake speed, greatly increase the total activity of endogenous enzymes in the digestive tract, and reduce stress diarrhea"

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A kind of preparation method of compound microbial fermented material is:

[0049] The present invention adopts strains and numbers are Lactobacillus casei (ACCC10640) Lactobacillus casei, Saccharomyces cerevisiae (IFO0203) brewer's yeast, Bacillus licheniformis (ACCC10619) Bacillus subtilis, Rhodopseudomonas palustris (ACCC10649) Rhodopseudomonas palustris, all bacterial strains Provided by the Culture Conservation Center of the Ministry of Agriculture.

[0050] Step 1: Breeding of Fermentation Strains

[0051] a. Subculture: Bacillus subtilis strains, Lactobacillus casei, Saccharomyces cerevisiae, and Rhodopseudomonas palustris (photosynthetic bacteria) provided by the Culture Conservation Center of the Ministry of Agriculture were used to subculture through nutrient agar medium, and Pseudomonas marsh Bacteria (photosynthetic bacteria) are cultivated under light conditions of 20°C on PDA medium, and the strains with fast growth and obvious colony characteristics are ...

Embodiment 2

[0073] The first step: breeding of fermented strains: same as Example 1

[0074] Step 2: Liquid high-speed culture of compound strains

[0075] a. Weigh each strain according to the following proportions: 24% of Bacillus subtilis, 27% of Lactobacillus casei, 35% of Saccharomyces cerevisiae, and 14% of Rhodopseudomonas palustris (photosynthetic bacteria).

[0076] B. Liquid culture of lactic acid bacteria and Saccharomyces cerevisiae: Rinse Lactobacillus casei and Saccharomyces cerevisiae by weighing the ratio of step a with aseptic physiological saline to make composite strain liquid, mix and carry out composite cultivation together: Inoculate the mixed bacterial lawn in the liquid medium sterilized at 125°C for 45 minutes at a ratio of 1:25, stir in the fermenter for 35 minutes at a speed of 220r / min, stir evenly and put it into a sterilized plastic bucket Stand still for 5-7 days, measure the Ph value every day and release the produced gas during the standing process; take ...

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Abstract

The invention relates to a composite microorganism ferment material which is characterized in that production strains select production strains of which the growth rate is quick and the enzyme production quantity is large by combining with through ray inducement and production performance detection. The processing technique employs innovative symbiosis compatibility design of probiotics and multi-compound fermentation technology, the number of the live probiotics in the products achieves five billion per gram, and the products simultaneously contain polyoses, polypeptide, organized enzyme, organic acid, phytosterin, triterpennoids saponin and the like active metabolite. Specific enzyme producing inducer is added to growth medium, the latest single creature venting valve is employed on the package, which not only can be added to complete feed of livestock or poultry but also can be directly added into feedstuff, thereby effectively promoting breakdown and absorption of giant molecule nutrient substance in the intestinal canal of livestock or poultry, thereby eliminating the action of anti-nutritional factors in the feedstuff, remarkably increasing utilization ratio of the feedstuff, and reducing the feeding cost.

Description

technical field [0001] The invention relates to a compound microbial fermentation material and a production method. It adopts probiotic symbiotic compatibility design and multi-stage compound fermentation technology, and each stage of fermentation has its own different target products, so that the product is rich in a large number of beneficial live bacteria, polysaccharides, and polypeptides. , active enzymes, organic acids, phytosterols, saponins and other active metabolites are more likely to be eaten, digested and absorbed by livestock and poultry, and belong to the technical field of agricultural biological products. Background technique [0002] Complete feed is also called complete diet compound feed. It refers to the protein, energy, trace elements, minerals and other ingredients that animals need for a certain period of growth, and can fully meet the nutritional needs of feeding objects. These feed materials are mainly composed of plant materials and animal fats, c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18A23K1/16C12R1/125C12R1/245C12R1/38A23K10/12
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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