Fermentation material for composite microorganism and preparation method thereof
A technology of compound microorganisms and fermented materials, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low enzyme production effect and total number of viable bacteria, unable to guarantee feed intake and feeding speed, etc. Achieving the effect of high feed nutrition
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Embodiment 1
[0048] A kind of preparation method of compound microbial fermented material is:
[0049] The present invention adopts strains and numbers are Lactobacillus casei (ACCC10640) Lactobacillus casei, Saccharomyces cerevisiae (IFO0203) brewer's yeast, Bacillus licheniformis (ACCC10619) Bacillus subtilis, Rhodopseudomonas palustris (ACCC10649) Rhodopseudomonas palustris, all bacterial strains Provided by the Culture Conservation Center of the Ministry of Agriculture.
[0050] Step 1: Breeding of Fermentation Strains
[0051] a. Subculture: Bacillus subtilis strains, Lactobacillus casei, Saccharomyces cerevisiae, and Rhodopseudomonas palustris (photosynthetic bacteria) provided by the Culture Conservation Center of the Ministry of Agriculture were used to subculture through nutrient agar medium, and Pseudomonas marsh Bacteria (photosynthetic bacteria) are cultivated under light conditions of 20°C on PDA medium, and the strains with fast growth and obvious colony characteristics are ...
Embodiment 2
[0073] The first step: breeding of fermented strains: same as Example 1
[0074] Step 2: Liquid high-speed culture of compound strains
[0075] a. Weigh each strain according to the following proportions: 24% of Bacillus subtilis, 27% of Lactobacillus casei, 35% of Saccharomyces cerevisiae, and 14% of Rhodopseudomonas palustris (photosynthetic bacteria).
[0076] B. Liquid culture of lactic acid bacteria and Saccharomyces cerevisiae: Rinse Lactobacillus casei and Saccharomyces cerevisiae by weighing the ratio of step a with aseptic physiological saline to make composite strain liquid, mix and carry out composite cultivation together: Inoculate the mixed bacterial lawn in the liquid medium sterilized at 125°C for 45 minutes at a ratio of 1:25, stir in the fermenter for 35 minutes at a speed of 220r / min, stir evenly and put it into a sterilized plastic bucket Stand still for 5-7 days, measure the Ph value every day and release the produced gas during the standing process; take ...
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