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Method for synthesizing and constructing reduction response type miRNA transportation system based on carbon dots

A construction method, responsive technology, applied in the direction of drug combination, pharmaceutical formula, organic active ingredients, etc.

Pending Publication Date: 2020-07-17
JIANGSU SYNTHGENE BIOTECHNOLOGY CO LTD
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  • Abstract
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Problems solved by technology

However, the application of carbon dots in the transport of miRNA has not been reported so far. Based on the previous research on the chemical modification of the 3' end of miRNA, combined with the properties of carbon dots, we designed a reduction-responsive miRNA transport system

Method used

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  • Method for synthesizing and constructing reduction response type miRNA transportation system based on carbon dots
  • Method for synthesizing and constructing reduction response type miRNA transportation system based on carbon dots
  • Method for synthesizing and constructing reduction response type miRNA transportation system based on carbon dots

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Embodiment Construction

[0035] The present invention will be further described in detail below in conjunction with specific embodiments.

[0036] 1. Related instruments and reagents

[0037] (1) Experimental equipment

[0038] NMR (1HNMR and 13CNMR): 400 MHz Bruker AVANCE III–400;

[0039] Ultraviolet spectrum detection instrument: Shimadzu UV-3600 ultraviolet spectrophotometer;

[0040] Fluorescence spectrum detection instrument: Shimadzu RF-5301PC fluorescence spectrometer;

[0041] Zeta potential and DLS detection instrument: Brookhaven 90Plus dynamic light scattering instrument;

[0042] TEM detection instrument: JEOL 2800 high-throughput transmission electron microscope;

[0043] High-resolution mass spectrometry detection instrument: Agilent 6550 iFunnel Q-TOF LC / MS;

[0044] High performance liquid chromatography: measured by Agilent 1200;

[0045] High performance liquid chromatography: Waters 2535 LC;

[0046] Nucleic acid synthesizer: German K&A series DNA / RNA / LNA nucleic acid synthesi...

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Abstract

The invention relates to a method for synthesizing and constructing a reduction response type miRNA transport system based on carbon dots. The miRNA is connected with carbon dots through disulfide bonds, and reducing species concentration difference inside and outside cells and reducing species concentration difference between cancer cells and normal cells are utilized, so that miRNA in the cancercells is selectively transported. The controllable release in the cancer cells is realized by utilizing the characteristic that the miRNA release speed of a transport system is low under the condition of extracellular low-concentration reducing species and the miRNA release speed is high in a reducing environment in the cancer cells; and meanwhile, adopted as carriers, the carbon dots have the advantages of good biocompatibility, small and uniform size, easy track due to fluorescence and the like. By means of the delivery system, intracellular transportation of miRNA can be efficiently achieved, and meanwhile, biological activity of the miRNA transported into cells can be kept. The technical scheme can provides the method for synthesizing and constructing the reduction response type miRNAtransport system with high carrying efficiency, convenient observation path and good representation performance based on carbon dots.

Description

technical field [0001] The invention relates to the technical field of drug delivery and controllable release, in particular to a method for the synthesis and construction of a reduction-responsive miRNA delivery system based on carbon dots. Background technique [0002] In recent years, chemical biology research targeting miRNAs has also gradually emerged, and most of the research focuses on the detection and regulation of miRNAs. Synthetic miRNA analogs (miRNA mimics) and miRNA inhibitors (miRNA inhibitors) have been proven to be effective bioactive macromolecules that regulate miRNA levels in organisms, and are increasingly used in the artificial regulation and innovative research of miRNA. However, due to the effect of negative charge, it is difficult for artificially synthesized miRNA analogs and miRNA inhibitors to enter the cell through the cell membrane. Therefore, it is necessary to develop transport vehicles to realize the intracellular transport of miRNA analogs a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/52A61K31/713A61P35/00
CPCA61K47/52A61K31/713A61P35/00
Inventor 童坤肖潇黄磊
Owner JIANGSU SYNTHGENE BIOTECHNOLOGY CO LTD
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