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Method for inducing precipitation of calcium carbonate by using microorganisms

A calcium carbonate and microorganism technology, applied in the field of using microorganisms to induce calcium carbonate precipitation, can solve the problems of reducing the reaction rate, waste of calcium resources, slow calcium ion sedimentation rate, etc., to achieve uniform precipitation, improved adsorption capacity, and good anti-seepage effect. Effect

Inactive Publication Date: 2020-07-14
CHINA UNIV OF GEOSCIENCES (BEIJING)
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Problems solved by technology

In this process, if the precipitation rate of calcium ions is slow or the amount of precipitation is too small, it will not only reduce the reaction rate, but also waste calcium resources.

Method used

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  • Method for inducing precipitation of calcium carbonate by using microorganisms
  • Method for inducing precipitation of calcium carbonate by using microorganisms
  • Method for inducing precipitation of calcium carbonate by using microorganisms

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Embodiment Construction

[0033] The present invention will be explained in more detail through specific embodiments below, but it is not intended to limit the protection scope of the present invention.

[0034] Bacillus pasteurianus: ATCC11859 (Accession No.), purchased.

[0035] Urea, Calcium Chloride (CaCl 2 ), sodium chloride (NaCl), casein peptone, and soybean peptone were purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd.; 752 UV-Vis Spectrophotometer was purchased from Shanghai Sunny Hengping Scientific Instrument Co., Ltd.

[0036] Sodium montmorillonite was purchased from Zhejiang Fenghong New Material Co., Ltd., and the particle size distribution of the sodium montmorillonite was as follows: figure 1 shown;

[0037] Suspension medium configuration: Add casein peptone (15g / L), soybean peptone (5g / L), NaCl (5g / L) and urea (20g / L) to 1000mL deionized water respectively, adjust the pH value to 7.3, Sterilize at 121°C for 30 minutes.

[0038] Preparation of Bacillus pasteurianus susp...

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Abstract

The invention provides a method for inducing precipitation of calcium carbonate by using microorganisms. The method comprises the following steps: mixing a bacterial suspension with a calcium reactionsolution to obtain a total gel solution, and adding sodium montmorillonite into the gel solution to carry out calcium carbonate precipitation, wherein the sodium montmorillonite accounts for 0.25-1%of the total gel solution by mass. According to the method, the sodium montmorillonite is added, and under the condition of interaction of the sodium montmorillonite, the reaction solution and urea, precipitation of calcium ions is accelerated, and the calcium ions can achieve the effect of complete precipitation, so that the reaction efficiency is obviously improved, and the method has a higher practical application value; and under the condition of more reaction solutions, the reaction can be carried out in the forward direction, the calcium ions are completely precipitated, and the precipitation amount of calcium carbonate in the reaction is obviously increased.

Description

technical field [0001] The invention relates to a method for using microorganisms to induce calcium carbonate precipitation. Background technique [0002] Microbe-induced calcium carbonate precipitation (MICP) is an innovative method for improving soil by urease-catalyzed hydrolysis of urea. With the continuous development of interdisciplinary research in microbiology, geochemistry, civil engineering and other disciplines, microbial-induced calcium carbonate Applied in various fields, this technology has a good effect on improving the strength and stability of soft soil foundations. [0003] At present, the research on MICP mainly focuses on three aspects. One is to focus on improving the strength and durability of cementitious materials through the biomineralization of MICP. MICP gels in situ through calcium-carbon precipitation; one focuses on the precipitation of calcium carbonate minerals induced by MICP in the form of three non-hydratable calcium carbonate crystals: ca...

Claims

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Application Information

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IPC IPC(8): C12P3/00B09C1/10C12R1/07
CPCB09C1/105B09C2101/00C12P3/00
Inventor 唐国旺王贵和贾苍琴王新杰唐俊
Owner CHINA UNIV OF GEOSCIENCES (BEIJING)
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