Construction method and application of children cough syrup fingerprint spectrum

A technology of cough syrup for children and fingerprints, which is applied to instruments, measuring devices, scientific instruments, etc., can solve the problems of lack of quality control of orange peel and platycodon grandiflorum, failure to achieve the quality evaluation of preparations, etc. Good, reduced dosage effect

Active Publication Date: 2020-07-10
YAAN THREE NINE PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The current quality control method for children's cough syrup in the "Chinese Pharmacopoeia" is mainly reflected in the content control of licorice and a...

Method used

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  • Construction method and application of children cough syrup fingerprint spectrum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] (a) Provide the test solution: take the children's cough syrup, accurately measure 1mL to a 10mL measuring bottle, add methanol to dilute to the mark, shake well, filter, and collect the subsequent filtrate as the test solution.

[0074] (b) Provide reference solution: take appropriate amount of ammonium glycyrrhizinate reference substance, hesperidin reference substance, platycodon saponin D reference substance, weigh them accurately, add 50% methanol to make a mixture containing 50 μg, 50 μg, and 20 μg per 1 mL respectively solution, that is.

[0075] (c) Accurately draw 1 μL each of the reference substance solution and the test solution, inject it into an ultra-high performance liquid chromatograph, and perform ultra-high performance liquid chromatography analysis to obtain the fingerprints of the reference substance and children's cough syrup, and perform ultra-high performance liquid chromatography analysis. The conditions are:

[0076] Octadecylsilane bonded sili...

Embodiment 2

[0086] Another 10 batches of 999 brand children's cough syrup produced in 2019 with different specifications were taken, measured according to the above-mentioned chromatographic conditions, compared with the control fingerprint and calculated the similarity. The results are shown in Table 1. The similarity is between 0.91 and 0.96. The fingerprint determination method constructed by the invention can be used for the quality control of children's cough syrup.

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Abstract

The invention provides a construction method and an application of a children cough syrup fingerprint spectrum, and relates to the technical field of pharmaceutical analysis. The construction method comprises the following steps: analyzing a sample to be detected by adopting ultra-high performance liquid chromatography, and obtaining a chromatographic peak of 0-15 min to obtain the children coughsyrup fingerprint spectrum, wherein analysis conditions of the ultra-high performance liquid chromatography are as follows: a mobile phase A includes an elution buffer solution, and a mobile phase B includes acetonitrile; a detection wavelength ranges from 208-212 nm in 0 min-12 min and ranges from 235-239 nm in 12 min-15 min; the mobile phase A is reduced from 95+/-5% v/v to 5+/-5% v/v in 0-15 min; and the mobile phase B is increased from 5+/-5% v/v to 95+/-5% v/v in 0-15 min. The construction method of the fingerprint spectrum can be used for identifying various chemical components in the children cough syrup at the same time, effectively saves detection time, and has very important significance for comprehensively evaluating quality of the children cough syrup.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for constructing fingerprints of cough syrup for children and its application. Background technique [0002] Pediatric cough syrup is a traditional Chinese medicine preparation, which is a reddish-brown translucent viscous liquid with a sweet taste. The main ingredients of children's cough syrup include licorice liquid extract, bellflower liquid extract, ammonium chloride and orange peel. Licorice is the root and rhizome of the leguminous plant licorice, which has the effects of eliminating phlegm and relieving cough, antitussive, clearing away heat and detoxification, invigorating the spleen and replenishing qi, relieving spasms and relieving pain; platycodon grandiflorum is the root of platycodon grandiflorum, the active ingredient contains saponin, platycodon grandiflorum has Xuanfei, expectorant, sore throat, pus drainage, replenishing qi and blood, regulating...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/32G01N30/34G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/32G01N30/34G01N30/74G01N30/8631G01N30/8686G01N2030/324
Inventor 袁海英蔡帮军侯新莲史琳莉
Owner YAAN THREE NINE PHARMA
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