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Kit for detecting 12 steroid hormones in serum by using ultra-high performance liquid chromatography-tandem mass spectrometry technology

An ultra-high performance liquid phase and steroid hormone technology, which can be used in measurement devices, instruments, scientific instruments, etc., and can solve the problems of high cost, limited clinical application, and complicated operation.

Active Publication Date: 2020-07-10
NANJING PINSHENG MEDICAL TECH CO LTD +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems in the detection of steroid hormones by the current LC-MS / MS technology. Solid-phase extraction or derivatization methods are mostly used for pretreatment, which is complicated to operate and high in cost, and its clinical application is relatively limited.

Method used

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  • Kit for detecting 12 steroid hormones in serum by using ultra-high performance liquid chromatography-tandem mass spectrometry technology
  • Kit for detecting 12 steroid hormones in serum by using ultra-high performance liquid chromatography-tandem mass spectrometry technology
  • Kit for detecting 12 steroid hormones in serum by using ultra-high performance liquid chromatography-tandem mass spectrometry technology

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0141] 1. Experimental materials and instruments

[0142] 1. Materials

[0143] The samples were obtained from serum samples collected in the outpatient clinic of Wuhan Asian Heart Hospital in March 2018.

[0144] (1) Instrument: Xevo TQ-S triple quadrupole mass spectrometer (Waters Corporation); UPLC I-Class ultra-high performance liquid chromatography system (with autosampler, Waters Corporation); SCILOGEX D2012 high-speed desktop centrifuge (USA) ; Ultrapure water instrument (ELGA LabWater, UK); Multi-tube vortex mixer (Vortex genie2, USA); Adjustable pipette (Eppendorf 0.5-10 μL, 10-100 μL, 100-1000 μL); glass instruments, measuring cylinders, etc. .

[0145] (2) Reagent consumables: MS grade methanol (Fisher, USA); HPLC grade methanol (Honeywell, USA); HPLC grade methyl tert-butyl ether (Fisher, USA); chromatographic column Kinetex XB-C18 (3.0×50mm, 2.6 μm) (Phenomenex, USA).

[0146] (3) Standard products: P4, 17α-OHP4, CORT, T, DHT, AD, E1 and F were purchased from ...

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Abstract

The invention relates to a kit for detecting 12 steroid hormones in serum by using an ultra-high performance liquid chromatography-tandem mass spectrometry technology. The kit is characterized in thata serum sample is mixed with a mixed internal standard solution of all to-be-detected substances, the sample does not need derivatization treatment, and a to-be-detected solution can be obtained justby one-step liquid-liquid extraction; electrolyte ammonium fluoride is added into a mobile phase, so that the ionization efficiency of certain target compounds can be effectively improved; and 12 hormones are simultaneously detected by adopting a mass spectrum positive-negative switching scanning mode. The kit has the advantages of high sensitivity, high specificity, low cost and simple and quickpretreatment process, does not need solid-phase extraction or derivation, can complete the separation and detection of 12 hormones within 5.0 minutes, and provides a reliable detection kit for the health assessment of clinical endocrine diseases.

Description

technical field [0001] The invention belongs to the technical field of blood detection, and in particular relates to a kit for detecting 12 steroid hormones in serum by ultra-high performance liquid chromatography tandem mass spectrometry. Background technique [0002] Steroid hormones are a class of fat-soluble small molecule hormones, also known as steroid hormones. They are produced by cholesterol through a series of enzyme-catalyzed metabolism, and play an important role in regulating the body's metabolism, immune regulation, sexual function regulation, growth and development, etc. Steroid hormones can be divided into progestins, mineralocorticoids, glucocorticoids, estrogens, and androgens according to their physiological functions. Progesterone, corticosterone, and aldosterone are mineralocorticoids; 17α-hydroxyprogesterone and cortisol are glucocorticoids; estrone and estradiol are estrogens; dehydroepiandrosterone sulfate, dehydroepiandrosterone, testosterone, Both...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/50
CPCG01N30/02G01N30/06G01N30/50G01N2030/045G01N2030/062
Inventor 成晓亮李美娟
Owner NANJING PINSHENG MEDICAL TECH CO LTD
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