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Method for measuring estradiol by adopting graphene multiple signal amplification SPR sensing

A multi-signal and graphene technology, applied in the direction of measuring devices, phase-influenced characteristic measurements, instruments, etc., can solve the problems of estradiol hazards, lower detection limits, accumulation, etc., to achieve increased sensitivity, lower detection limits, and high sensitivity Effect

Active Publication Date: 2020-07-10
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because estradiol is extremely harmful and can accumulate in the human body, people hope to achieve ultra-trace detection of estradiol, and how to further reduce the detection limit is also a common goal of relevant researchers

Method used

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  • Method for measuring estradiol by adopting graphene multiple signal amplification SPR sensing
  • Method for measuring estradiol by adopting graphene multiple signal amplification SPR sensing
  • Method for measuring estradiol by adopting graphene multiple signal amplification SPR sensing

Examples

Experimental program
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Effect test

Embodiment 1

[0114] This embodiment does not include the pretreatment step of enriching estradiol in the sample to be tested by using magnetic graphene. This embodiment includes the following steps:

[0115] Step 1: Decorate the gold surface of the SPR chip with graphene

[0116] (1) Put the gold sheet in the newly configured piranha solution (30% H 2 0 2 :H 2 S0 4 =1:3) Soak at room temperature for 1-2 hours to remove the organic matter on the surface of the gold sheet, then wash with a large amount of deionized water, wash with absolute ethanol, and finally blow dry with nitrogen.

[0117] (2) After filtering the 0.01mol / L MPA ethanol solution, place it in a petri dish, then immerse the gold flakes in the solution to avoid air bubbles on the gold surface, and place them overnight; rinse the gold flakes with ethanol three times to remove excess MPA; Rinse three times with deionized water to remove excess alcohol; blow dry the gold flakes with nitrogen gas.

[0118] (3) Soak the MPA-...

Embodiment 2

[0138] This embodiment is on the basis of Example 1, also adopts the pretreatment that the estradiol in the sample to be tested is enriched by magnetic graphene, and the sample to be tested is obtained after the treatment, and the pretreatment is specifically:

[0139] The enrichment conditions were optimized by high performance liquid chromatography, and 30 mg of magnetic graphene was used to treat E under the condition of pH 5-6. 2 The estradiol in the standard sample was adsorbed for 5 minutes, and after filtration, the magnetic graphene was eluted with 2 mL of acetonitrile for 5 minutes, blown dry with nitrogen gas and adjusted to 200 μL, as the sample to be tested.

[0140] Other steps and conditions are carried out with reference to Example 1.

[0141] The experimental results showed that after introducing the pretreatment step of magnetic graphene enrichment, the surface plasmon resonance biosensor modified with carboxylated graphene oxide was used to detect E 2 , moni...

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Abstract

The invention relates to a method for measuring estradiol by adopting graphene multiple signal amplification SPR sensing. The method comprises the following steps: S1, sample preparation: adsorbing and enriching estradiol in a to-be-measured sample by using magnetic graphene as an adsorbent, eluting with an eluent, and fixing the volume of the eluted estradiol to obtain the to-be-measured sample;S2, cleaning the surface of a noble metal sheet of the SPR chip and connecting the noble metal sheet with carboxylated graphene oxide; S3, connecting and fixing the E2-BSA by utilizing carboxylated graphene oxide fixed on the precious metal surface of the SPR chip; S4, determining the concentration of estradiol in the to-be-tested sample by utilizing SPR immunosensing based on an indirect competition method: mixing the to-be-tested sample containing E2 and the monoclonal antibody solution of E2 for sample introduction, jointly competing for E2-BSA fixed on the surface of the SPR chip, and a signal generated by SPR being inversely proportional to the concentration of E2 in the to-be-tested sample. According to the estradiol content determination method, the LOD value is as low as 3.605 * 10<-6> ng / mL, the detection limit is reduced by more than 3400 times, and ultra-trace detection of estradiol can be realized.

Description

technical field [0001] The invention relates to the technical field of detection of endocrine interference, in particular to a method for measuring estradiol by using graphene multiple signal amplification SPR sensing. Background technique [0002] With the improvement of people's living standards, people pay more and more attention to environmental hormones, and more and more researches focus on food-derived hormones and human health. The content of estrogen in food-derived substances has a great impact on people's health. Especially milk, the main intake of milk is children. There is a considerable amount of estrogen in milk, and some scholars believe that the consumption of milk is significantly higher than that of 100 years ago. According to research, estradiol can cause abnormal development and function of the male reproductive system, and in severe cases can cause serious diseases such as hypospadias, gonadal atrophy and testicular cancer, and exogenous estradiol can...

Claims

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Application Information

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IPC IPC(8): G01N21/41G01N21/03G01N33/577G01N33/543
CPCG01N21/41G01N21/03G01N33/577G01N33/54366G01N33/54393G01N2021/0325
Inventor 高志贤李双白家磊彭媛宁保安王江韩殿鹏任汉林周焕英
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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