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Neutral monoclonal antibodies for resisting GDF15 and application of neutral monoclonal antibodies for resisting GDF15

A technology of monoclonal antibody and variable region, applied in the field of biomedicine

Active Publication Date: 2020-07-10
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are not many reports on the role of GDF15 in the development of inflammation and the impact on immune cells, and further research is still needed in combination with environmental factors related to chronic inflammatory responses and acute inflammatory responses

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  • Neutral monoclonal antibodies for resisting GDF15 and application of neutral monoclonal antibodies for resisting GDF15

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Embodiment Construction

[0050] In order to enable those skilled in the art to better understand the solutions of the present invention, the following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only It is an embodiment of a part of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0051] It should be noted that the terms "comprising" and "having" and any variations thereof in the description and claims of the present invention are intended to cover non-exclusive inclusion, for example, processes, methods, A system, product or device is not necessarily limited to those steps or elements explicitly listed, but ...

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Abstract

The invention discloses two neutral monoclonal antibodies for resisting GDF15 and an application of the neutral monoclonal antibodies for resisting GDF15, and provides variable regions of a light chain and a heavy chain of antibodies G1 and G2, having neutralizing activity. Through a hybridoma technique, monoclonal antibodies for resisting GDF15 of mice are prepared, GDF15 resisting hybridoma cellstrains which can stably secrete high specificity antibodies can be screened, and high-specificity and high-affinity GDF15 resisting monoclonal antibodies are obtained by preparing ascites. A simpleand stable antibody neutralization detection system is constructed, and two GDF15 monoclonal antibodies having neutralizing activity are verified for the first time. Based on research on immunomodulation effect of GDF15, a lymphocyte and tumor cell co-culture system and a mouse-borne tumor model are established to verify inhibition of the neutral monoclonal antibodies on tumor growth based on in vitro and in vivo levels, and foundation is laid for an application of tumor immunotherapy, metabolism correlation diseases and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to monoclonal antibodies, especially two anti-GDF15 neutralizing monoclonal antibodies and applications thereof. Background technique [0002] Growth differentiation factor 15 (Growth differentiation factor 15, GDF15) is one of the distal members of the transforming growth factor β (Transforming growth factor β, TGF-β) superfamily, also known as macrophage inhibitory factor-1 (MIC-1), Placental bone morphogenetic protein (PLAB), placental transforming growth factor beta (PTGFβ), non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1), prostate-derived growth factor (PDF), etc. The level of GDF15 is very low under normal physiological conditions, but the level of GDF15 increases significantly when cardiovascular, tumor and inflammation-related diseases occur, and is closely related to the poor prognosis of the disease. [0003] 1. Molecular characteristics of GDF15 [0004] ...

Claims

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Application Information

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IPC IPC(8): C07K16/22C12N15/13A61K39/395A61P35/00A61P3/04A61P1/14A61P3/00
CPCC07K16/22A61P35/00A61P3/04A61P1/14A61P3/00C07K2317/76C07K2317/565C07K2317/56C07K2317/92C07K2317/73A61K2039/505
Inventor 张英起李萌徐传洋王昭维李维娜何磊张存郝强赵宁
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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