Photosensitive composite bio-ink for 3D printing and preparation method thereof

A bio-ink and 3D printing technology, applied in the field of biomaterials and cell-containing hydrogels, can solve the problems of low viscosity, large amount of modifiers, and limited cell proliferation.

Active Publication Date: 2021-09-10
SHENYANG INST OF AUTOMATION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But both have no printability due to low viscosity
[0004] Photosensitive gelatin is a widely used printing material in recent years. Although its printability is good, its gel network is too tight and cell proliferation is limited.
In addition, the traditional synthesis method uses a large amount of modifiers, the preparation process has a large odor, low yield, and slow dialysis

Method used

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  • Photosensitive composite bio-ink for 3D printing and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The raw material of the collagen solution is self-extracted bovine Achilles tendon collagen, which is obtained through degreasing, enzymatic hydrolysis, salting out and other steps. The bovine Achilles tendon glue is dissolved in 0.1mol / L acetic acid solution to prepare an acidic collagen solution of 6mg / mL .

[0038] Mix 8.09 mL of the above collagen solution, 1 mL of 10-fold concentration PBS, 0.81 mL of 1 mol / L sodium hydroxide, and 0.1 mL of 1 mg / mL phenol red. At this point, the solution was poured into 10 times the volume of 0.01 mol / L PBS solution, and magnetically stirred in a water bath at 37° C. for 1 hour at a stirring speed of 1000 rpm. The above solution was centrifuged at 10000 g for 1 h, the supernatant was discarded, and the precipitate was collagen fibers. The above steps are performed aseptically.

[0039] Adopt improved synthesis method of photosensitive gelatin: weigh 10g of gelatin, dissolve in 100mL of PBS, heat to 60°C, stir until completely disso...

Embodiment 2

[0043] The raw material for the preparation of the collagen solution was self-extracted bovine Achilles tendon collagen. After degreasing, enzymatic hydrolysis, salting out and other steps, an 8mg / mL acidic collagen solution was obtained. The solvent was acetic acid solution with a concentration of 0.1mol / L. Mix 8.09 mL of the above collagen solution, 1 mL of 10-fold concentration PBS, 0.81 mL of 1 mol / L sodium hydroxide, and 0.1 mL of 1 mg / mL phenol red. At this time, the solution was poured into 5 times volume of 2 times concentration PBS solution, and magnetically stirred in a water bath at 25° C. for 2 hours at a stirring speed of 1000 rpm. The above solution was centrifuged at 10000 g for 1 h, the supernatant was discarded, and the precipitate was collagen fibers. The above steps are performed aseptically. A modified photosensitive gelatin synthesis method was used.

[0044] Weigh 10g of gelatin, dissolve it in 100mL of PBS, heat to 60°C, stir until completely dissolved...

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Abstract

The invention discloses a photosensitive composite biological ink for 3D printing and a preparation method thereof, and belongs to the technical field of biological materials and cell-containing hydrogels. The ink has temperature-sensitive and light-sensitive properties, and the properties of this double gel are very conducive to the formation of 3D printing. The ink has two components, the first being collagen fibers. Studies have shown that mesenchymal stem cells can differentiate into osteoblasts more effectively on the surface of collagen fibers. The second component is photosensitive gelatin, which retains the temperature-sensitive properties of gelatin and has fast light-curing performance, which is especially suitable for precise 3D printing accumulation. In particular, the combination of the two will produce a superimposed effect, the collagen fibers have changed the original gel structure of the photosensitive gelatin, and the cells have stronger proliferation and extension capabilities in the composite gel of this phase-separated structure. Furthermore, the ink was shown to promote the differentiation of mesenchymal stem cells into osteoblasts.

Description

technical field [0001] The invention relates to the technical field of biological materials and cell-containing hydrogels, in particular to a photosensitive composite biological ink for 3D printing and a preparation method thereof. Background technique [0002] 3D printing is an emerging manufacturing technology, which refers to the use of three-dimensional scanning and other means to build a three-dimensional model of the structure, and then quickly print out various structures through the precise accumulation of materials. At present, the printing technology of traditional materials such as metal materials and plastics has been widely used in production and life. Bioprinting technology specifically refers to the use of biocompatible materials to construct printed structures, which can replace human tissues or organs to achieve accurate and rapid personalized repair. [0003] At present, products such as meninges and bone defect substitutes have been used in clinical pract...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/38A61L27/48A61L27/58
CPCA61L27/3834A61L27/48A61L27/52A61L27/58A61L2430/02C08L89/00
Inventor 郑雄飞郭凯王赫然李松朱慧轩
Owner SHENYANG INST OF AUTOMATION - CHINESE ACAD OF SCI
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