Human BCR/ABL gene T315I mutation detection and analysis
A technology for humans, testing methods, applied in the field of molecular biology, which can solve problems such as ineffectiveness
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[0022] The mutation detection and analysis of the human BCR / ABLT315I gene is based on the principle of amplification block mutation, which can accurately detect the mutant DNA with a content as low as 0.5%, that is, only 100 copies of the mutant can be detected; specific detection In the background of a higher copy of the wild plasmid, there is still no false positive.
[0023] The ABL T315I mutation of the BCR-ABL fusion gene is clinically used as the main indicator for CML and Ph+ALL drug treatment guidance, and has important guiding significance for the screening of CML and Ph+ALL drug resistance and clinical scientific medication.
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