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Total flavone extract with anti-depression effect and preparation method and application thereof

A technology of total flavonoids and extracts, applied in the field of medicine, can solve problems such as side effects, and achieve the effect of simple steps, significant antidepressant activity, and high extraction efficiency

Active Publication Date: 2020-07-03
宁夏超仁健康管理科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these drugs can play a certain antidepressant effect, they will produce a series of side effects along with their drug effects; and a survey shows that about one-third of antidepressant patients are forced to stop taking the drug because they cannot tolerate the side effects of the drug

Method used

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  • Total flavone extract with anti-depression effect and preparation method and application thereof
  • Total flavone extract with anti-depression effect and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 A kind of preparation method of the total flavone extract with antidepressant effect, specifically comprises the following steps:

[0033] (1) According to the solid-to-liquid ratio of 1:8, add ethanol with a volume concentration of 95% to the dodder medicinal material, heat and reflux extraction for 3 times, each time for 2 hours, filter and combine the extracts, and concentrate under reduced pressure at 65°C until there is no alcohol smell;

[0034] (2) Get the concentrate in step (1) and carry out separation and purification on a polyamide chromatographic column, elute with ethanol solution with volume concentration of 30% and 70% respectively, and collect ethanol elution with volume concentration of 30% and 70% liquid, mixed and concentrated to obtain a concentrated solution.

[0035] (3) Add water to the concentrated solution obtained in step (2), stir and dilute it, then let it stand for 6-8h, and centrifuge (3000-5000rpm, 30min) to collect the precip...

Embodiment 2

[0037] Embodiment 2 A kind of preparation method of the total flavone extract with antidepressant effect, specifically comprises the following steps:

[0038] (1) According to the solid-to-liquid ratio of 1:8, add ethanol with a volume concentration of 95% to the dodder medicinal material, heat and reflux extraction for 3 times, each time for 2 hours, filter and combine the extracts, and concentrate under reduced pressure at 65°C until there is no alcohol smell;

[0039] (2) Get the concentrate in step (1) and carry out separation and purification on a polyamide chromatographic column, elute with ethanol solution with volume concentration of 40% and 80% respectively, and collect ethanol elution with volume concentration of 40% and 80% liquid, mixed and concentrated to obtain a concentrated solution.

[0040] (3) Add water to the concentrated solution obtained in step (2), stir and dilute it, then let it stand for 6-8h, and centrifuge (3000-5000rpm, 30min) to collect the precipit...

Embodiment 3

[0042] Embodiment 3 antidepressant experiment

[0043] 1. The modeling method of chronic stress-stimulated depressed mice in this example is:

[0044] Before the depression modeling, the experimental mice were given 2% sugar water for 3 days to adapt. After 3 days, the mice in the normal group were reared separately from the mice in the other groups. Food and water should be fasted during the sugar water preference experiment, and they were free to eat at other times. The model animals were subjected to chronic mild stimulation for 3 weeks, including swimming in cold water (15°C, 6min); tail clamping (1min); water deprivation (24h); fasting (24h); °); wet rearing (100ml water was added to the bottom of the cage with sawdust); overnight lighting stimulation. That is, the mice received one stimulus every day, each stimulus was guaranteed to be carried out randomly but not continuously, and the number of overnight lighting stimulations was at least twice a week.

[0045] 2. St...

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Abstract

The present invention discloses total flavone extract with the anti-depression effect and a preparation method and application thereof. The total flavone extract is prepared by the following preparation method of adding an extraction solvent into cuscuta chinensis lam. powder, performing heating reflux extraction, combining filtrate, and performing concentration to obtain extractum; and sequentially adding the extractum into a polyamide column and a macroporous resin column for chromatography, and concentrating obtained eluent to obtain the total flavone extract of cuscuta chinensis lam.. Theprepared total flavone extract is high in purity, experimental results show that total flavone of the cuscuta chinensis lam. can obviously shorten dead time of CUMS mice TST and FST and does not affect autonomic activities of the mice, and it is indicated that the total flavone extract of the cuscuta chinensis lam. has the relatively strong anti-depression effect and no central excitation or inhibition effect. Moreover, the total flavone of the cuscuta chinensis lam. can activate a TXNIP / NLRP3 inflammasome signal path, and it is proved that the total flavone of the cuscuta chinensis lam. exerts the anti-depression effect by adjusting the TXNIP / NLRP3 inflammasome signal path.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a total flavonoid extract with antidepressant effect and its preparation method and application. Background technique [0002] Depression is one of the most common mental illnesses. It is characterized by significant and persistent depression, often accompanied by serious psychological and physical disorders, and is extremely harmful to human health. The world invests a lot in its research and development every year, bringing great benefits to the society. serious burden on the family. In addition, patients with depression are often accompanied by changes in eating patterns related to insomnia, lethargy, overeating, weight loss or anorexia, and more serious symptoms include some extreme symptoms such as circadian rhythm and endocrine disorders. At present, there are at least 90 million depression patients in my country, and only 5% of them have received treatment; at the same t...

Claims

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Application Information

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IPC IPC(8): A61K36/39A61K36/43A61P25/24A23L33/105
CPCA61K36/39A61K36/43A61P25/24A23L33/105A61K2236/333A61K2236/39A61K2236/55A61K2236/51A23V2002/00A23V2200/322A23V2250/2116
Inventor 王汉卿佟月唐蓉蓉陶伟伟刘颖赵建军雍婧姣王庆贺凯张霞冯军王建寰高晓娟付慧玲柳全霞曾亮杨彩艳
Owner 宁夏超仁健康管理科技有限公司
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