Immobilized microorganisms for sewage treatment as well as preparation method and application of immobilized microorganisms

A technology for immobilized microorganisms and sewage treatment, applied in biological water/sewage treatment, water/sludge/sewage treatment, chemical instruments and methods, etc., can solve the problems of reactor loss and achieve short growth stagnation time and microbial loss The effect of reducing and strengthening the denitrification effect

Inactive Publication Date: 2020-06-30
ZHEJIANG SHUANGLIANG SUNDA ENVIRONMENTAL PROTECTION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In traditional wastewater biological treatment processes, microorganisms usually grow in suspension in the water and are therefore easily lost from the reactor

Method used

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  • Immobilized microorganisms for sewage treatment as well as preparation method and application of immobilized microorganisms
  • Immobilized microorganisms for sewage treatment as well as preparation method and application of immobilized microorganisms
  • Immobilized microorganisms for sewage treatment as well as preparation method and application of immobilized microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Dissolve 4% chitosan in 1% acetic acid solution, and carry out high-density culture of simultaneous nitrification and denitrification bacteria, mix the chitosan mixture with the bacteria solution and drop it into the sodium hydroxide solution to cross-link into immobilized pellets. The prepared immobilized microorganisms were added to a conical flask containing 100mL of sewage, and cultured in a shaker at 37°C and 100r / min. Three days later, another batch of sewage was replaced, and the pellets in the conical flask were not change, repeat this step until the ball can no longer be used. Regular sampling and testing of ammonia nitrogen and total nitrogen are used to study the utilization rate and denitrification effect of immobilized microorganisms. For measurement results, please refer to figure 1 shown. After measurement and observation, a total of 9 batches of experiments were carried out, showing that the immobilized bacteria have a longer-term denitrification effe...

Embodiment 2

[0046]The immobilized microorganisms prepared in Example 1 were added to a 5L simple simulated OA process for application evaluation. The experiment uses the same water source, that is, the total nitrogen (TN) in the water is equal, and one group is added with immobilized microorganisms, and the volume ratio is 3kg / 1m 3 , the other group was added with free microorganisms, and the effluent TN of the two groups of purification tanks were measured respectively. Please refer to figure 2 It was shown that the degradation rate of free microorganisms increased rapidly, but the degradation process of immobilized bacteria lasted longer, and the degradation efficiency was generally higher than that of free microorganisms.

Embodiment 3

[0048] The immobilized microorganisms prepared in Example 1 were added to a 5L simple simulated OA process for application evaluation. The experiment uses the same water source, that is, the total nitrogen (TN) in the water is equal, and one group is added with immobilized microorganisms, and the volume ratio is 3kg / 1m 3 , the other group was added with film-coated fillers to immobilize microorganisms, and the effluent TN of the two groups of purification tanks were measured respectively. Please refer to figure 2 It can be seen that the bacteria with film-coated fillers are not ideal for the degradation of total nitrogen in sewage, and the immobilized bacteria have outstanding advantages compared with them.

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Abstract

The present application discloses immobilized microorganisms for sewage treatment. The immobilized microorganisms include functional microorganisms and immobilized carrier chitosan, wherein the functional microorganisms are simultaneous nitrification and denitrification bacteria, and the immobilized carrier chitosan is a biopolymer obtained by deacetylation of chitin. The invention also disclosesa preparation method and application of the above immobilized microorganisms for sewage treatment. According to the method, the functional microorganisms and the immobilized carrier are mixed to formsolid microbial bacteria pellets, wherein the immobilized carrier chitosan can be used as an adsorbent to adsorb nitrogen, phosphorus and other elements in sewage, and the functional microorganisms has an excellent removal effect on nitrogen in the sewage. When the immobilized microorganisms are used, the immobilized microorganisms can be put into a sewage treatment facility, so that the operationis simple.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to an immobilized microorganism used for sewage treatment and its preparation method and application. Background technique [0002] With the development of the economy and the progress of the society, the population in rural areas has gradually increased, and the way of life has undergone tremendous changes. In recent years, heavy polluting enterprises and factories have gradually become marginalized. Agricultural sewage and industrial wastewater will flow into the domestic sewage pipe network through the washing of rainwater. , causing domestic sewage pollution in rural areas to gradually intensify. Nitrogen pollution in rural domestic sewage is the primary problem in the treatment of rural domestic sewage pollution. Nitrogen can lead to eutrophication of water bodies, causing rapid growth of algae in water bodies, rapidly consuming oxygen in water, and causing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C02F3/34C02F3/30
CPCC02F3/302C02F3/34C12N11/10Y02W10/40
Inventor 郑展望潘碧文汪啸斯东浩李列飞彭义文崔志文
Owner ZHEJIANG SHUANGLIANG SUNDA ENVIRONMENTAL PROTECTION CO LTD
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