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Extraction method of gypenoside XLIX and medicine for treating diabetes and hyperlipidemia

A technology of Gynostemma saponins and Gynostemma Gynostemma saponins is applied in the field of medicine and chemical industry, and can solve the problems of short clinical use time and high cost, and achieve the effect of obvious effect and improved cure rate.

Pending Publication Date: 2020-06-30
徐世明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used drugs to stimulate insulin secretion include sulfonylurea secretagogues and meglitinide secretagogue drugs, which are expensive and have a short clinical use time, and the clinical efficacy and safety need to be further verified

Method used

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  • Extraction method of gypenoside XLIX and medicine for treating diabetes and hyperlipidemia
  • Extraction method of gypenoside XLIX and medicine for treating diabetes and hyperlipidemia
  • Extraction method of gypenoside XLIX and medicine for treating diabetes and hyperlipidemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 10kg of total saponins of Gynostemma pentaphylla, dissolve in methanol, mix the sample with 20kg of 100-mesh silica gel, evaporate the solvent, put on 120kg of 100-mesh dry-process silica gel chromatography column, use chloroform-methanol mixed solution, and the ratio of the mixed solution is from 8:1→3:1 Carry out gradient elution, cooperate with thin-layer chromatography inspection, use the upper layer of n-butanol-acetic acid-water mixed solution with a ratio of 4:1:5 as the developer, and use 10% sulfuric acid ethanol solution as the color developer to develop color , collect and combine the eluate containing gypenoside XLIX, and recover the solvent to obtain the crude product of gypenoside XLIX.

[0035] Take the crude gypenoside XLIX, prepare a 150 mg / ml solution with methanol, inject it into a preparative high-performance liquid phase, use octadecylsilane bonded silica gel with a particle size of 20 μm as the filler, and use methanol:water=70:30 as The mobil...

Embodiment 2

[0039] Take 15kg of total saponins of Gynostemma pentaphyllum, dissolve in methanol, mix the sample with 35kg of 160-mesh silica gel, evaporate the solvent, put on 90kg of 160-mesh dry-process silica gel chromatography column, use chloroform-methanol mixed solution, the ratio of the mixed solution is from 8:1→3:1 Carry out gradient elution, cooperate with thin-layer chromatography inspection, use the upper layer of n-butanol-acetic acid-water mixed solution with a ratio of 4:1:5 as the developer, and use 10% sulfuric acid ethanol solution as the color developer to develop color , collect and combine the eluate containing gypenoside XLIX, and recover the solvent to obtain the crude product of gypenoside XLIX.

[0040] Take the crude Gypenoside XLIX, prepare a 200mg / ml solution with methanol, inject it into the preparative high performance liquid phase, use octadecylsilane bonded silica gel with a particle size of 25 μm as the filler, and mix methanol:water=70:30 The solution is...

Embodiment 3

[0044] Take 10kg of total saponins of Gynostemma pentaphyllum, dissolve in methanol, mix the sample with 15kg of 200 mesh silica gel, evaporate the solvent, put on 80kg of 200 mesh dry silica gel chromatography column, use chloroform-methanol mixed solution, the ratio of the mixed solution is from 8:1→3:1 Carry out gradient elution, cooperate with thin-layer chromatography inspection, use the upper layer of n-butanol-acetic acid-water mixed solution with a ratio of 4:1:5 as the developer, and use 10% sulfuric acid ethanol solution as the color developer to develop color , collect and combine the eluate containing gypenoside XLIX, and recover the solvent to obtain the crude product of gypenoside XLIX.

[0045] Take the crude gypenoside XLIX, prepare a 250mg / ml solution with methanol, inject it into a preparative high-efficiency liquid phase, use octadecylsilane bonded silica gel with a particle size of 25 μm as the filler, and use methanol:water=70:30 as The mobile phase was us...

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Abstract

The invention discloses an extraction method of gypenoside XLIX and a medicine for treating diabetes and hyperlipidemia, belongs to the technical field of pharmaceutical chemicals, and provides a medicine taking gypenoside XLIX which is extracted and purified from gynostemma pentaphylla total saponins as a single active component, and applications of the extracted gypenoside XLIX in preparation ofthe medicine used for treating patients with diabetes and cardiovascular diseases. The medical curative effect is definite, the medicine is safe and free of toxic and side effects. The method for extracting gypenoside XLIX comprises the following steps: by taking gynostemma pentaphylla total saponins as a raw material, carrying out column chromatography, recovering a solvent to obtain a gypenoside XLIX crude product, dissolving the crude product with methanol, purifying by adopting preparative liquid chromatography, and repeatedly recrystallizing to obtain gypenoside XLIX with the purity of 90% or higher. The medicine for treating diabetes and hyperlipidemia is prepared by adding medical auxiliary materials into gypenoside XLIX with the content of 90% or higher. According to the method, amodern extraction and purification technology is adopted, and the purity of the extracted gypenoside XLIX reaches 90% or above; the medicine prepared from the gypenoside XLIX is used for treating diseases including type I diabetes mellitus, type II diabetes mellitus and diabetic nephropathy.

Description

technical field [0001] The invention belongs to the technical field of medicine and chemical industry, in particular to a method for extracting gypenoside XLIX and a medicine for treating diabetes and hyperlipidemia. Background technique [0002] Diabetes is a chronic metabolic disease that seriously endangers human health and has a high prevalence in my country. Diabetes (diabetes) is caused by various pathogenic factors such as genetic factors, immune dysfunction, microbial infection and its toxins, free radical toxins, and mental factors acting on the body, resulting in hypofunction of the body's islets and insulin resistance, thereby triggering the body's sugar , protein, fat, water and electrolytes and a series of metabolic disorder syndromes. Typical cases may show symptoms such as polyuria, polydipsia, polyphagia, and weight loss, that is, "three excesses and one deficiency". Once diabetes (blood sugar) is not well controlled, it will cause complications, leading to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00A61K31/704A61P3/10A61P3/06A61P13/12A61P9/10A61P1/10A61P29/00A61P9/12A61P1/00
CPCC07J17/005A61P3/10A61P3/06A61P13/12A61P9/10A61P1/10A61P29/00A61P9/12A61P1/00
Inventor 徐世明高海彭勇
Owner 徐世明
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