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Method for degrading vomitoxin in deep processing process of corn

A technology of vomitoxin and deep processing, which is applied in the field of deep processing of corn, can solve the problems of excessive vomitoxin content in corn by-products, excessive vomitoxin content, and vomitoxin pollution, etc., and achieves the effect of significant detoxification effect, alleviation of utilization problems and significant economic benefits.

Active Publication Date: 2020-06-26
JILIN COFCO BIOCHEM +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Excessive vomitoxin is the main problem of grain processing-related products at present: in the process of deep processing of corn starch, due to the water-soluble characteristics of vomitoxin, it dissolves in corn steep liquor during the soaking process of corn sulfurous acid, and corn bran and germ meal, which are corn by-products, need to be sprayed. slurry to increase its protein content, causing the content of vomitoxin in sprayed corn husks and corn germ meal to exceed the standard seriously
However, due to the serious pollution of corn raw material DON in the corn deep processing industry at present, and the existing production methods cannot effectively degrade DON (also known as detoxification), the content of DON in corn by-products exceeds the standard, and most of the existing biological detoxification methods are used in In the process of grain processing, there is a problem that the efficiency is significantly reduced under high temperature load or harsh acid-base conditions

Method used

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  • Method for degrading vomitoxin in deep processing process of corn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] The live thallus of Bacillus subtilis whose preservation number is CGMCC NO.14597 is in 30mL LB liquid culture medium (10g peptone, 5g yeast powder, 10g sodium chloride, deionized water is settled to 1L, adjusts pH to 7, through 121 ℃ sterilization for 15 min), shake culture overnight at 180 rpm and 37 ℃ for activation. Then, inoculate in 60mL LB liquid culture medium with the inoculation amount of 1v / v%, shake culture under the condition of 180rpm, 37 ℃ until the bacterium concentration in the bacterial liquid is (1±0.1)×10 5 CFU / mL, the fermentation broth was centrifuged at 8000rpm for 10min, and the bacteria were removed to obtain the supernatant, which was to prepare the crude enzyme solution. The crude enzyme solution prepared above was mixed with 200mL factory corn steep liquor (30wt% dry solids content, pH 5.0) at a volume ratio of 1:100, adjusted to pH 7 with sodium hydroxide, and fully mixed and reacted at 37°C. Samples were taken before the reaction (0h) and ...

Embodiment 2

[0091] The crude enzyme solution prepared in Example 1 was added into 200 mL factory corn steep liquor (dry solid content 50 wt%, pH 3.0) at a volume ratio of 1:100, and mixed and reacted at 50° C. for 24 hours. Samples were taken before and after the reaction to detect the content of vomitoxin. It is calculated that the degradation rate of vomitoxin can reach 43.8%.

[0092] The results of Example 1-Example 2 illustrate that the method of the present invention not only has a significant degradation effect under neutral mild conditions (37°C, pH7.0), but also at higher temperatures (50°C) and lower Under pH (pH 3), it can also obviously degrade the vomitoxin in corn steep liquor, which proves that the method of the present invention can overcome most of the existing biological method detoxification and be applied to the higher temperature or harsh acid-base conditions in the grain processing process The problem that the detoxification efficiency is significantly reduced under...

Embodiment 3

[0094]The fermentation broth was obtained in the same manner as in Example 1, the fermentation broth was centrifuged at 8000 rpm for 10 min, and the bacteria were collected and freeze-dried to prepare a freeze-dried microbial agent. Mix 200g of mildewed corn with 200mL of 50°C tap water, soak at a constant temperature of 50°C for 2 hours, filter and recover the corn to obtain a corn soaking solution (pH 5.0), use sodium hydroxide to adjust the pH of the corn soaking solution to 7.0 and cool to 37°C, and then add the above-mentioned freeze-dried bacterial agent into it at a ratio of 2w / v%, mix well, and react at 37°C and pH 7.0 for 24h. Samples were taken before and after the reaction to detect the content of vomitoxin. The calculated degradation rate of DON can reach 85.6%.

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Abstract

The invention relates to the field of deep processing of corn and in particular relates to a method for degrading vomitoxin in the deep processing process of corn. The method comprises the following step: mixing bacillus subtilis of which the preservation number is CGMCC NO.14597 or a product which is prepared from the bacillus subtilis and has vomitoxin degradation activity with a byproduct in the deep processing process of the corn. In the deep processing process of corn starch, the byproduct in the deep processing process of the corn, particularly corn steep liquor and a corn soaking liquid, is subjected to vomitoxin detoxification, a remarkable vomitoxin detoxification effect on the byproduct and a finally obtained gunited feed product can be achieved, and the vomitoxin degradation rate can be up to 40% or greater; and in addition, the method provided by the invention is capable of overcoming the problem that the detoxification efficiency of most conventional biological method detoxification applied to high temperatures or harsh acid-base conditions in the cereal processing process is remarkably reduced, and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of corn deep processing, in particular to a method for degrading vomitoxin in the deep processing of corn, and the method is aimed at by-products in the deep processing of corn, especially corn steep liquor and corn soaking liquid. Background technique [0002] The deep processing of corn (starch) is a process in which the main components of corn are separated by soaking, crushing, separating, drying and other processes in sulfuric acid to obtain corresponding products. Through this process, five main by-products can be obtained: sprayed corn bran, corn gluten meal, corn germ meal, corn crude oil and final product starch. [0003] Vomitoxin (vomitoxin) is also known as deoxynivalenol (deoxynivalenol, DON), the chemical name is 3α, 7α, 15-trihydroxy Fusarium-9-en-8-one, because it can cause vomiting in pigs It is named after the trichothecenes mainly produced by Fusarium graminearum and Fusarium culmorum inf...

Claims

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Application Information

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IPC IPC(8): A23L5/20
CPCA23L5/25A23L5/28Y02P60/87
Inventor 佟毅谭剑唐堂黄锦李义吴延东张媛王靖陈博赵雪松
Owner JILIN COFCO BIOCHEM
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