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Raising method of Spodoptera frugiperda larvae

A technology of Spodoptera frugiperda and larvae, which is applied in the field of raising Spodoptera frugiperda larvae, can solve the problems of increasing workload, the number of breeding utensils and breeding sites, difficulty in meeting large-scale feeding, and low feeding efficiency, so as to benefit Group breeding, good for climbing and molting, and superior insect rearing efficiency

Inactive Publication Date: 2020-06-26
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The artificial breeding technology is complex and costly, and feed and breeding equipment need to be replaced every day, which greatly increases the workload in the larva rearing process, the number of breeding equipment and the breeding site, the feeding efficiency is low, and it is difficult to meet the requirements of large-scale breeding

Method used

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  • Raising method of Spodoptera frugiperda larvae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) Formulated feed

[0053] The feed formula is: 1000g of water, 100g of soybean powder, 60g of yeast powder, 100g of corn flour, 5g of corn oil, 2g of sorbic acid, 2g of methyl p-hydroxybenzoate, 4g of ascorbic acid, 0.8g of cholesterol, 1.4g of formaldehyde solution, and 16g of agar.

[0054] (2) Feeding method:

[0055] With a diameter of 9.8 cm and a height of 4.1 cm in a sterilized plastic box, after Spodoptera litura was raised to the third instar, the larvae that were too small were picked out, and the larvae that grew evenly were kept, and 30 larvae were raised in each box. Pin the plastic box with 8 small holes with paper clips, cover it with crepe roll paper first, then cover the plastic box cover, put it at 30°C, 70%-80% relative humidity and light time L:D=16:8h Raise under raising condition, when treating that larva is close to pupation, transfer to the feeding device that the sandy loam soil of 12% soil water content is housed to pupate. Management of p...

Embodiment 2

[0057] (1) Formulated feed

[0058] The feed formula is: 1000g of water, 100g of soybean powder, 60g of yeast powder, 100g of corn flour, 5g of corn oil, 2g of sorbic acid, 4g of ascorbic acid, 2g of methyl p-hydroxybenzoate, 0.8g of cholesterol, 1.4g of formaldehyde solution, and 16g of agar.

[0059] (2) Feeding method:

[0060] With a diameter of 9.8 cm and a height of 4.1 cm in a sterilized plastic box, after Spodoptera frugiperda was raised to the third instar, the larvae that were too small were picked out, and the larvae that grew evenly were kept, and 25 larvae were raised in each box. Pin the plastic box with 8 small holes with paper clips, cover it with crepe roll paper first, then cover the plastic box cover, put it at 30°C, 70%-80% relative humidity and light time L:D=16:8h Raise under raising condition, when treating that larva is close to pupation, transfer to the feeding device that the sandy loam soil of 12% soil water content is housed to pupate. Management ...

Embodiment 3

[0062] (1) Formulated feed

[0063] The feed formula is: 1000g of water, 100g of soybean powder, 60g of yeast powder, 100g of corn flour, 5g of corn oil, 2g of sorbic acid, 4g of ascorbic acid, 2g of methyl p-hydroxybenzoate, 0.8g of cholesterol, 1.4g of formaldehyde solution, and 16g of agar.

[0064] (2) Feeding method:

[0065] With a diameter of 9.8 cm and a height of 4.1 cm in a sterilized plastic box, after Spodoptera frugiperda was raised to the third instar, the too small larvae were picked out, and the larvae with uniform growth were kept, and 20 larvae were raised in each box. Pin the plastic box with 8 small holes with paper clips, cover it with crepe roll paper first, then cover the plastic box cover, put it at 30°C, 70%-80% relative humidity and light time L:D=16:8h Raise under feeding conditions, and when the larvae are close to pupation, transfer to a feeding device with sandy loam with 10% soil water content to pupate. Management of pupae: put the mature larv...

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Abstract

The invention provides a raising method of Spodoptera frugiperda larvae, and belongs to the technical field of insect raising. The raising method comprises the following steps that raising the Spodoptera frugiperda larvae in a raising device under the conditions of 28-30 DEG C, light time of 16 h / d, light intensity of 15%-25%, and humidity of 70%-80% until the larvae being old and mature to obtainold and mature larvae. The raising device is a raising device with a cover, and a wrinkle roll paper is covered between the cover of the raising device and a cover opening of the raising device. Using the method of the present invention to feed Spodoptera frugiperda, the five indicators of Spodoptera frugiperda survival rate, pupation rate, feathering rate, average pupal weight and egg productionare close to the raising results of single-head raising, thereby being an effective solution to solve the technical problems of self-killing during raising of Spodoptera frugiperda larvae. The raising method of the invention is easy to operate, and the insect raising efficiency is much better than a single-head raising efficiency, thereby making raising of Spodoptera frugiperda in groups a reality.

Description

technical field [0001] The invention relates to the technical field of insect feeding, in particular to a method for feeding Spodoptera frugiperda larvae. Background technique [0002] Spodoptera frugiperda belongs to Lepidoptera Noctuidae and is one of the most important pests worldwide. In order to reduce the harm of fall armyworm to crops and realize the effective control of fall armyworm, it is urgent to study the biological characteristics, ecology and drug resistance of fall armyworm, as well as to screen chemical pesticides and biological pesticides and toxicity testing. In order to carry out the above-mentioned research work, it is first necessary to carry out long-term large-scale breeding of Spodoptera frugiperda indoors, so as to obtain consistent standardization of insect age, high-quality and large-scale experimental insects, and also provide a basis for large-scale insect breeding. As the main virus biopesticide, industrialization provides a large number of a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A23K50/90A23K10/30A23K10/18A23K20/158A23K20/174A23K20/168A23K20/163A23L3/3508A23L3/3517A23L3/3499
CPCA01K67/033A23L3/3499A23L3/3508A23L3/3517A23K10/18A23K10/30A23K20/158A23K20/163A23K20/168A23K20/174A23K50/90Y02A40/90
Inventor 刘旭刘昌华陈庆东刘虹伶陈松刘月悦李超郝员敏
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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