A kind of method of constructing flower red cotyledon regeneration system

A technology of flower red cotyledon and system, applied in plant regeneration, horticultural methods, botany equipment and methods, etc., can solve problems such as difficult rooting and regeneration, and achieve more regenerated buds, stronger regenerated buds, and conducive to transplanting and survival Effect

Active Publication Date: 2022-05-24
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method of using cotyledons as explants to induce differentiation into roots or buds, and to construct a regeneration system for cotyledons, which overcomes the shortcomings of the original regeneration system and solves the problems of difficult regeneration and rooting.

Method used

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  • A kind of method of constructing flower red cotyledon regeneration system
  • A kind of method of constructing flower red cotyledon regeneration system
  • A kind of method of constructing flower red cotyledon regeneration system

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Embodiment 1

[0039] A method for constructing a red flower cotyledon regeneration system, using the red flower red cotyledons as explants to complete in vitro regeneration, comprising the following steps:

[0040] (1) Pretreatment: select mature and plump flower red seeds and soak them in sterile water for 10 minutes;

[0041] (2) Sterilization: Soak the above-mentioned seeds with 75% alcohol for 30 seconds, rinse them with sterile water for 2-3 times, then soak them with 10% sodium hypochlorite solution for 10 minutes, and rinse them with sterile water for 3-4 times;

[0042] (3) Adventitious bud induction: after the sterilized seeds were stripped of the germ, after cutting off some cotyledons at the proximal end and the distal end, they were inoculated on the adventitious bud induction medium, cultured in the dark for 7 days, and then cultured under light;

[0043] Adventitious bud induction medium components are: MS+1mg / LTDZ+0.05mg / LNAA;

[0044] (4) elongate strong bud cultivation: tr...

Embodiment 2

[0050] A method for constructing a red flower cotyledon regeneration system, using the red flower red cotyledons as explants to complete in vitro regeneration, specifically includes the following steps:

[0051] (1) Pretreatment: select plump red seeds and soak them in sterile water for 10 minutes;

[0052] (2) Sterilization: wash with 75% alcohol for 30 seconds, wash with sterile water for 2-3 times, then treat with 10% sodium hypochlorite solution for 10 minutes, and wash with sterile water for 3-4 times;

[0053] (3) Adventitious bud induction: the sterilized seeds are stripped of the germ, cut off some cotyledons at the proximal end and the distal end, and inoculated on the adventitious bud induction medium, and cultivated under light;

[0054] Adventitious bud induction medium components are: MS+1mg / LTDZ+0.05mg / LNAA;

[0055](4) elongate strong bud cultivation: transfer step (3) culture material to elongate strong bud culture medium, cultivate under light for 30-40 days;...

Embodiment 3

[0061] A method for constructing a red flower cotyledon regeneration system, using the red flower red cotyledons as explants to complete in vitro regeneration, specifically includes the following steps:

[0062] (1) Pretreatment: select plump red seeds and soak them in sterile water for 10 minutes;

[0063] (2) Sterilization: wash with 75% alcohol for 30 seconds, wash with sterile water for 2-3 times, then treat with 10% sodium hypochlorite solution for 10 minutes, and wash with sterile water for 3-4 times;

[0064] (3) Adventitious bud induction: the sterilized seeds are stripped of the germ, cut off some cotyledons at the proximal end and the distal end, and inoculated on the adventitious bud induction medium, cultured in the dark for 14 days, and then cultured under light;

[0065] Adventitious bud induction medium components are: MS+1mg / LTDZ+0.05mg / LNAA;

[0066] (4) elongate strong bud cultivation: transfer step (3) culture material to elongate strong bud culture medium,...

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Abstract

The invention discloses a method for constructing a red cotyledon regeneration system, comprising the following steps: 1. pretreatment; 2. sterilization; 3. induction of adventitious buds; 4. cultivation of elongated and strong buds; plant. In the present invention, the cotyledon of the flower red is used as the explant to complete the in vitro regeneration, and the technical scheme provided by the invention is used to perform the in vitro regeneration of the flower red, which improves the induced germination rate and the value-added coefficient. The test proves that the regenerated seedlings obtained in the present invention have budding With the advantages of robustness, healthy taproot, and high survival rate of transplanted seedlings, the method for constructing a red cotyledon regeneration system provided by the invention provides a new technology and method for genetic transformation and molecular breeding of red flowers.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for constructing a red cotyledon regeneration system. Background technique [0002] Flower red (Malus asiatica), Rosaceae (Rosaceae), Malus (Malus), deciduous small trees. Huahong, also known as "Shaguo" and "Lin'ao", is flat in nature, sweet and sour in taste, rich in various nutrients, and has the effects of quenching thirst, promoting body fluid, and eliminating stagnation. In addition to eating fresh, the fruit can also be processed into dried fruit, fruit skin or wine. Huahong is not only an ornamental tree species with beautiful flowers and fruits, but also an excellent rootstock for apples because of its good grafting affinity with apples. However, Huahong is mainly propagated by grafting, and the severe deforestation has led to a serious shortage of germplasm resources in Huahong. This problem can be solved by establishing a regeneratio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 文晓鹏邹思艳田文杰李孝绒
Owner GUIZHOU UNIV
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